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Involvement of CD26 in Differentiation and Functions of Th1 and Th17 Subpopulations of T Lymphocytes
CD26, acting as a costimulator of T cell activation, plays an important role in the immune system. However, the role of CD26 in the differentiation of T cell subsets, especially of new paradigms of T cells, such as Th17 and Tregs, is not fully clarified. In the present study, the role of CD26 in T c...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7843192/ https://www.ncbi.nlm.nih.gov/pubmed/33542930 http://dx.doi.org/10.1155/2021/6671410 |
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author | Zhao, Xiangli Wang, Wenhan Zhang, Kai Yang, Jingya Fuchs, Hendrik Fan, Hua |
author_facet | Zhao, Xiangli Wang, Wenhan Zhang, Kai Yang, Jingya Fuchs, Hendrik Fan, Hua |
author_sort | Zhao, Xiangli |
collection | PubMed |
description | CD26, acting as a costimulator of T cell activation, plays an important role in the immune system. However, the role of CD26 in the differentiation of T cell subsets, especially of new paradigms of T cells, such as Th17 and Tregs, is not fully clarified. In the present study, the role of CD26 in T cell differentiation was investigated in vitro. CD26 expression was analyzed in the different subsets of human peripheral blood T lymphocytes after solid-phase immobilized specific anti-CD3 mAb stimulation. Here, the percentage of CD4(+) cells significantly increased and most of these cells were coexpressed with CD26, suggesting a close correlation of CD26 expression with the proliferation of CD4(+) cells. Subsequently, after immobilized anti-CD3 mAb stimulation, CD26 high-expressing cells (CD26(high)) were separated from CD26 low-expressing cells (CD26(low)) by magnetic cell sorting. We found that the percentages of cells secreting Th1 typical cytokines (IL-2, IFN-γ) and Th17 typical cytokines (IL-6, IL-17, and IL-22) or expressing Th17 typical biomarkers (IL-23R, CD161, and CD196) in the CD26(high) group were markedly higher than in those in the CD26(low) group. In addition, a coexpression of CD26 with IL-2, IFN-γ, IL-17, IL-22, and IL-23R in lymphocytes was demonstrated by fluorescence microscopy. These results provide direct evidence that the high expression of CD26 is accompanied by the differentiation of T lymphocytes into Th1 and Th17, indicating that CD26 plays a crucial role in regulating the immune response. |
format | Online Article Text |
id | pubmed-7843192 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Hindawi |
record_format | MEDLINE/PubMed |
spelling | pubmed-78431922021-02-03 Involvement of CD26 in Differentiation and Functions of Th1 and Th17 Subpopulations of T Lymphocytes Zhao, Xiangli Wang, Wenhan Zhang, Kai Yang, Jingya Fuchs, Hendrik Fan, Hua J Immunol Res Research Article CD26, acting as a costimulator of T cell activation, plays an important role in the immune system. However, the role of CD26 in the differentiation of T cell subsets, especially of new paradigms of T cells, such as Th17 and Tregs, is not fully clarified. In the present study, the role of CD26 in T cell differentiation was investigated in vitro. CD26 expression was analyzed in the different subsets of human peripheral blood T lymphocytes after solid-phase immobilized specific anti-CD3 mAb stimulation. Here, the percentage of CD4(+) cells significantly increased and most of these cells were coexpressed with CD26, suggesting a close correlation of CD26 expression with the proliferation of CD4(+) cells. Subsequently, after immobilized anti-CD3 mAb stimulation, CD26 high-expressing cells (CD26(high)) were separated from CD26 low-expressing cells (CD26(low)) by magnetic cell sorting. We found that the percentages of cells secreting Th1 typical cytokines (IL-2, IFN-γ) and Th17 typical cytokines (IL-6, IL-17, and IL-22) or expressing Th17 typical biomarkers (IL-23R, CD161, and CD196) in the CD26(high) group were markedly higher than in those in the CD26(low) group. In addition, a coexpression of CD26 with IL-2, IFN-γ, IL-17, IL-22, and IL-23R in lymphocytes was demonstrated by fluorescence microscopy. These results provide direct evidence that the high expression of CD26 is accompanied by the differentiation of T lymphocytes into Th1 and Th17, indicating that CD26 plays a crucial role in regulating the immune response. Hindawi 2021-01-20 /pmc/articles/PMC7843192/ /pubmed/33542930 http://dx.doi.org/10.1155/2021/6671410 Text en Copyright © 2021 Xiangli Zhao et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Zhao, Xiangli Wang, Wenhan Zhang, Kai Yang, Jingya Fuchs, Hendrik Fan, Hua Involvement of CD26 in Differentiation and Functions of Th1 and Th17 Subpopulations of T Lymphocytes |
title | Involvement of CD26 in Differentiation and Functions of Th1 and Th17 Subpopulations of T Lymphocytes |
title_full | Involvement of CD26 in Differentiation and Functions of Th1 and Th17 Subpopulations of T Lymphocytes |
title_fullStr | Involvement of CD26 in Differentiation and Functions of Th1 and Th17 Subpopulations of T Lymphocytes |
title_full_unstemmed | Involvement of CD26 in Differentiation and Functions of Th1 and Th17 Subpopulations of T Lymphocytes |
title_short | Involvement of CD26 in Differentiation and Functions of Th1 and Th17 Subpopulations of T Lymphocytes |
title_sort | involvement of cd26 in differentiation and functions of th1 and th17 subpopulations of t lymphocytes |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7843192/ https://www.ncbi.nlm.nih.gov/pubmed/33542930 http://dx.doi.org/10.1155/2021/6671410 |
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