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In silico and in vitro Evaluation of Mimetic Peptides as Potential Antigen Candidates for Prophylaxis of Leishmaniosis
Antigen formulation is the main feature for the success of leishmaniosis diagnosis and vaccination, since the disease is caused by different parasite species that display particularities which determine their pathogenicity and virulence. It is desirable that the antigens are recognized by different...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7843434/ https://www.ncbi.nlm.nih.gov/pubmed/33520931 http://dx.doi.org/10.3389/fchem.2020.601409 |
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author | Guedes, Deborah Carbonera Santiani, Manuel Hospinal Carvalho, Joyce Soccol, Carlos Ricardo Minozzo, João Carlos Machado de Ávila, Ricardo Andrez de Moura, Juliana Ferreira Ramos, Eliezer Lucas Pires Castro, Guillermo Raul Chávez-Olórtegi, Carlos Thomaz-Soccol, Vanete |
author_facet | Guedes, Deborah Carbonera Santiani, Manuel Hospinal Carvalho, Joyce Soccol, Carlos Ricardo Minozzo, João Carlos Machado de Ávila, Ricardo Andrez de Moura, Juliana Ferreira Ramos, Eliezer Lucas Pires Castro, Guillermo Raul Chávez-Olórtegi, Carlos Thomaz-Soccol, Vanete |
author_sort | Guedes, Deborah Carbonera |
collection | PubMed |
description | Antigen formulation is the main feature for the success of leishmaniosis diagnosis and vaccination, since the disease is caused by different parasite species that display particularities which determine their pathogenicity and virulence. It is desirable that the antigens are recognized by different antibodies and are immunogenic for almost all Leishmania species. To overcome this problem, we selected six potentially immunogenic peptides derived from Leishmania histones and parasite membrane molecules obtained by phage display or spot synthesis and entrapped in liposome structures. We used these peptides to immunize New Zealand rabbits and determine the immunogenic capacity of the chimeric antigen. The peptides induced the production of antibodies as a humoral immune response against L. braziliensis or L. infantum. Next, to evaluate the innate response to induce cellular activation, macrophages from the peptide mix-immunized rabbits were infected in vitro with L. braziliensis or L. infantum. The peptide mix generated the IFN-γ, IL-12, IL-4 and TGF-β that led to Th1 and Th2 cellular immune responses. Interestingly, this mix of peptides also induced high expression of iNOS. These results suggest that the mix of peptides derived from histone and parasites membrane molecules was able to mimic parasites proteins and induce cytokines important to CD4+ T cell Th1 and Th2 differentiation and effector molecule to control the parasite infection. Finally, this peptide induced an immune balance that is important to prevent immunopathological disorders, inflammatory reactions, and control the parasite infection. |
format | Online Article Text |
id | pubmed-7843434 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-78434342021-01-30 In silico and in vitro Evaluation of Mimetic Peptides as Potential Antigen Candidates for Prophylaxis of Leishmaniosis Guedes, Deborah Carbonera Santiani, Manuel Hospinal Carvalho, Joyce Soccol, Carlos Ricardo Minozzo, João Carlos Machado de Ávila, Ricardo Andrez de Moura, Juliana Ferreira Ramos, Eliezer Lucas Pires Castro, Guillermo Raul Chávez-Olórtegi, Carlos Thomaz-Soccol, Vanete Front Chem Chemistry Antigen formulation is the main feature for the success of leishmaniosis diagnosis and vaccination, since the disease is caused by different parasite species that display particularities which determine their pathogenicity and virulence. It is desirable that the antigens are recognized by different antibodies and are immunogenic for almost all Leishmania species. To overcome this problem, we selected six potentially immunogenic peptides derived from Leishmania histones and parasite membrane molecules obtained by phage display or spot synthesis and entrapped in liposome structures. We used these peptides to immunize New Zealand rabbits and determine the immunogenic capacity of the chimeric antigen. The peptides induced the production of antibodies as a humoral immune response against L. braziliensis or L. infantum. Next, to evaluate the innate response to induce cellular activation, macrophages from the peptide mix-immunized rabbits were infected in vitro with L. braziliensis or L. infantum. The peptide mix generated the IFN-γ, IL-12, IL-4 and TGF-β that led to Th1 and Th2 cellular immune responses. Interestingly, this mix of peptides also induced high expression of iNOS. These results suggest that the mix of peptides derived from histone and parasites membrane molecules was able to mimic parasites proteins and induce cytokines important to CD4+ T cell Th1 and Th2 differentiation and effector molecule to control the parasite infection. Finally, this peptide induced an immune balance that is important to prevent immunopathological disorders, inflammatory reactions, and control the parasite infection. Frontiers Media S.A. 2021-01-15 /pmc/articles/PMC7843434/ /pubmed/33520931 http://dx.doi.org/10.3389/fchem.2020.601409 Text en Copyright © 2021 Guedes, Santiani, Carvalho, Soccol, Minozzo, Machado de Ávila, de Moura, Ramos, Castro, Chávez-Olórtegi and Thomaz-Soccol. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Chemistry Guedes, Deborah Carbonera Santiani, Manuel Hospinal Carvalho, Joyce Soccol, Carlos Ricardo Minozzo, João Carlos Machado de Ávila, Ricardo Andrez de Moura, Juliana Ferreira Ramos, Eliezer Lucas Pires Castro, Guillermo Raul Chávez-Olórtegi, Carlos Thomaz-Soccol, Vanete In silico and in vitro Evaluation of Mimetic Peptides as Potential Antigen Candidates for Prophylaxis of Leishmaniosis |
title | In silico and in vitro Evaluation of Mimetic Peptides as Potential Antigen Candidates for Prophylaxis of Leishmaniosis |
title_full | In silico and in vitro Evaluation of Mimetic Peptides as Potential Antigen Candidates for Prophylaxis of Leishmaniosis |
title_fullStr | In silico and in vitro Evaluation of Mimetic Peptides as Potential Antigen Candidates for Prophylaxis of Leishmaniosis |
title_full_unstemmed | In silico and in vitro Evaluation of Mimetic Peptides as Potential Antigen Candidates for Prophylaxis of Leishmaniosis |
title_short | In silico and in vitro Evaluation of Mimetic Peptides as Potential Antigen Candidates for Prophylaxis of Leishmaniosis |
title_sort | in silico and in vitro evaluation of mimetic peptides as potential antigen candidates for prophylaxis of leishmaniosis |
topic | Chemistry |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7843434/ https://www.ncbi.nlm.nih.gov/pubmed/33520931 http://dx.doi.org/10.3389/fchem.2020.601409 |
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