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In silico and in vitro Evaluation of Mimetic Peptides as Potential Antigen Candidates for Prophylaxis of Leishmaniosis

Antigen formulation is the main feature for the success of leishmaniosis diagnosis and vaccination, since the disease is caused by different parasite species that display particularities which determine their pathogenicity and virulence. It is desirable that the antigens are recognized by different...

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Autores principales: Guedes, Deborah Carbonera, Santiani, Manuel Hospinal, Carvalho, Joyce, Soccol, Carlos Ricardo, Minozzo, João Carlos, Machado de Ávila, Ricardo Andrez, de Moura, Juliana Ferreira, Ramos, Eliezer Lucas Pires, Castro, Guillermo Raul, Chávez-Olórtegi, Carlos, Thomaz-Soccol, Vanete
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7843434/
https://www.ncbi.nlm.nih.gov/pubmed/33520931
http://dx.doi.org/10.3389/fchem.2020.601409
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author Guedes, Deborah Carbonera
Santiani, Manuel Hospinal
Carvalho, Joyce
Soccol, Carlos Ricardo
Minozzo, João Carlos
Machado de Ávila, Ricardo Andrez
de Moura, Juliana Ferreira
Ramos, Eliezer Lucas Pires
Castro, Guillermo Raul
Chávez-Olórtegi, Carlos
Thomaz-Soccol, Vanete
author_facet Guedes, Deborah Carbonera
Santiani, Manuel Hospinal
Carvalho, Joyce
Soccol, Carlos Ricardo
Minozzo, João Carlos
Machado de Ávila, Ricardo Andrez
de Moura, Juliana Ferreira
Ramos, Eliezer Lucas Pires
Castro, Guillermo Raul
Chávez-Olórtegi, Carlos
Thomaz-Soccol, Vanete
author_sort Guedes, Deborah Carbonera
collection PubMed
description Antigen formulation is the main feature for the success of leishmaniosis diagnosis and vaccination, since the disease is caused by different parasite species that display particularities which determine their pathogenicity and virulence. It is desirable that the antigens are recognized by different antibodies and are immunogenic for almost all Leishmania species. To overcome this problem, we selected six potentially immunogenic peptides derived from Leishmania histones and parasite membrane molecules obtained by phage display or spot synthesis and entrapped in liposome structures. We used these peptides to immunize New Zealand rabbits and determine the immunogenic capacity of the chimeric antigen. The peptides induced the production of antibodies as a humoral immune response against L. braziliensis or L. infantum. Next, to evaluate the innate response to induce cellular activation, macrophages from the peptide mix-immunized rabbits were infected in vitro with L. braziliensis or L. infantum. The peptide mix generated the IFN-γ, IL-12, IL-4 and TGF-β that led to Th1 and Th2 cellular immune responses. Interestingly, this mix of peptides also induced high expression of iNOS. These results suggest that the mix of peptides derived from histone and parasites membrane molecules was able to mimic parasites proteins and induce cytokines important to CD4+ T cell Th1 and Th2 differentiation and effector molecule to control the parasite infection. Finally, this peptide induced an immune balance that is important to prevent immunopathological disorders, inflammatory reactions, and control the parasite infection.
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spelling pubmed-78434342021-01-30 In silico and in vitro Evaluation of Mimetic Peptides as Potential Antigen Candidates for Prophylaxis of Leishmaniosis Guedes, Deborah Carbonera Santiani, Manuel Hospinal Carvalho, Joyce Soccol, Carlos Ricardo Minozzo, João Carlos Machado de Ávila, Ricardo Andrez de Moura, Juliana Ferreira Ramos, Eliezer Lucas Pires Castro, Guillermo Raul Chávez-Olórtegi, Carlos Thomaz-Soccol, Vanete Front Chem Chemistry Antigen formulation is the main feature for the success of leishmaniosis diagnosis and vaccination, since the disease is caused by different parasite species that display particularities which determine their pathogenicity and virulence. It is desirable that the antigens are recognized by different antibodies and are immunogenic for almost all Leishmania species. To overcome this problem, we selected six potentially immunogenic peptides derived from Leishmania histones and parasite membrane molecules obtained by phage display or spot synthesis and entrapped in liposome structures. We used these peptides to immunize New Zealand rabbits and determine the immunogenic capacity of the chimeric antigen. The peptides induced the production of antibodies as a humoral immune response against L. braziliensis or L. infantum. Next, to evaluate the innate response to induce cellular activation, macrophages from the peptide mix-immunized rabbits were infected in vitro with L. braziliensis or L. infantum. The peptide mix generated the IFN-γ, IL-12, IL-4 and TGF-β that led to Th1 and Th2 cellular immune responses. Interestingly, this mix of peptides also induced high expression of iNOS. These results suggest that the mix of peptides derived from histone and parasites membrane molecules was able to mimic parasites proteins and induce cytokines important to CD4+ T cell Th1 and Th2 differentiation and effector molecule to control the parasite infection. Finally, this peptide induced an immune balance that is important to prevent immunopathological disorders, inflammatory reactions, and control the parasite infection. Frontiers Media S.A. 2021-01-15 /pmc/articles/PMC7843434/ /pubmed/33520931 http://dx.doi.org/10.3389/fchem.2020.601409 Text en Copyright © 2021 Guedes, Santiani, Carvalho, Soccol, Minozzo, Machado de Ávila, de Moura, Ramos, Castro, Chávez-Olórtegi and Thomaz-Soccol. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Chemistry
Guedes, Deborah Carbonera
Santiani, Manuel Hospinal
Carvalho, Joyce
Soccol, Carlos Ricardo
Minozzo, João Carlos
Machado de Ávila, Ricardo Andrez
de Moura, Juliana Ferreira
Ramos, Eliezer Lucas Pires
Castro, Guillermo Raul
Chávez-Olórtegi, Carlos
Thomaz-Soccol, Vanete
In silico and in vitro Evaluation of Mimetic Peptides as Potential Antigen Candidates for Prophylaxis of Leishmaniosis
title In silico and in vitro Evaluation of Mimetic Peptides as Potential Antigen Candidates for Prophylaxis of Leishmaniosis
title_full In silico and in vitro Evaluation of Mimetic Peptides as Potential Antigen Candidates for Prophylaxis of Leishmaniosis
title_fullStr In silico and in vitro Evaluation of Mimetic Peptides as Potential Antigen Candidates for Prophylaxis of Leishmaniosis
title_full_unstemmed In silico and in vitro Evaluation of Mimetic Peptides as Potential Antigen Candidates for Prophylaxis of Leishmaniosis
title_short In silico and in vitro Evaluation of Mimetic Peptides as Potential Antigen Candidates for Prophylaxis of Leishmaniosis
title_sort in silico and in vitro evaluation of mimetic peptides as potential antigen candidates for prophylaxis of leishmaniosis
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7843434/
https://www.ncbi.nlm.nih.gov/pubmed/33520931
http://dx.doi.org/10.3389/fchem.2020.601409
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