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A Labeling Strategy for Living Specimens in Long-Term/Super-Resolution Fluorescence Imaging
Despite the urgent need to image living specimens for cutting-edge biological research, most existing fluorescent labeling methods suffer from either poor optical properties or complicated operations required to realize cell-permeability and specificity. In this study, we introduce a method to overc...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7843436/ https://www.ncbi.nlm.nih.gov/pubmed/33520932 http://dx.doi.org/10.3389/fchem.2020.601436 |
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author | Han, Yubing Zhang, Zhimin Liu, Wenjie Yao, Yuanfa Xu, Yingke Liu, Xu Kuang, Cuifang Hao, Xiang |
author_facet | Han, Yubing Zhang, Zhimin Liu, Wenjie Yao, Yuanfa Xu, Yingke Liu, Xu Kuang, Cuifang Hao, Xiang |
author_sort | Han, Yubing |
collection | PubMed |
description | Despite the urgent need to image living specimens for cutting-edge biological research, most existing fluorescent labeling methods suffer from either poor optical properties or complicated operations required to realize cell-permeability and specificity. In this study, we introduce a method to overcome these limits—taking advantage of the intrinsic affinity of bright and photostable fluorophores, no matter if they are supposed to be live-cell incompatible or not. Incubated with living cells and tissues in particular conditions (concentration and temperature), some Atto and BODIPY dyes show live-cell labeling capability for specific organelles without physical cell-penetration or chemical modifications. Notably, by using Atto 647N as a live-cell mitochondrial marker, we obtain 2.5-time enhancement of brightness and photostability compared with the most commonly used SiR dye in long-term imaging. Our strategy has expanded the scientist's toolbox for understanding the dynamics and interactions of subcellular structures in living specimens. |
format | Online Article Text |
id | pubmed-7843436 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-78434362021-01-30 A Labeling Strategy for Living Specimens in Long-Term/Super-Resolution Fluorescence Imaging Han, Yubing Zhang, Zhimin Liu, Wenjie Yao, Yuanfa Xu, Yingke Liu, Xu Kuang, Cuifang Hao, Xiang Front Chem Chemistry Despite the urgent need to image living specimens for cutting-edge biological research, most existing fluorescent labeling methods suffer from either poor optical properties or complicated operations required to realize cell-permeability and specificity. In this study, we introduce a method to overcome these limits—taking advantage of the intrinsic affinity of bright and photostable fluorophores, no matter if they are supposed to be live-cell incompatible or not. Incubated with living cells and tissues in particular conditions (concentration and temperature), some Atto and BODIPY dyes show live-cell labeling capability for specific organelles without physical cell-penetration or chemical modifications. Notably, by using Atto 647N as a live-cell mitochondrial marker, we obtain 2.5-time enhancement of brightness and photostability compared with the most commonly used SiR dye in long-term imaging. Our strategy has expanded the scientist's toolbox for understanding the dynamics and interactions of subcellular structures in living specimens. Frontiers Media S.A. 2021-01-15 /pmc/articles/PMC7843436/ /pubmed/33520932 http://dx.doi.org/10.3389/fchem.2020.601436 Text en Copyright © 2021 Han, Zhang, Liu, Yao, Xu, Liu, Kuang and Hao. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Chemistry Han, Yubing Zhang, Zhimin Liu, Wenjie Yao, Yuanfa Xu, Yingke Liu, Xu Kuang, Cuifang Hao, Xiang A Labeling Strategy for Living Specimens in Long-Term/Super-Resolution Fluorescence Imaging |
title | A Labeling Strategy for Living Specimens in Long-Term/Super-Resolution Fluorescence Imaging |
title_full | A Labeling Strategy for Living Specimens in Long-Term/Super-Resolution Fluorescence Imaging |
title_fullStr | A Labeling Strategy for Living Specimens in Long-Term/Super-Resolution Fluorescence Imaging |
title_full_unstemmed | A Labeling Strategy for Living Specimens in Long-Term/Super-Resolution Fluorescence Imaging |
title_short | A Labeling Strategy for Living Specimens in Long-Term/Super-Resolution Fluorescence Imaging |
title_sort | labeling strategy for living specimens in long-term/super-resolution fluorescence imaging |
topic | Chemistry |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7843436/ https://www.ncbi.nlm.nih.gov/pubmed/33520932 http://dx.doi.org/10.3389/fchem.2020.601436 |
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