Cargando…

Generation of SARS-CoV-2 Spike Pseudotyped Virus for Viral Entry and Neutralization Assays: A 1-Week Protocol

The COVID-19 pandemic caused by the SARS-CoV-2 coronavirus requires reliable assays for studying viral entry mechanisms which remains poorly understood. This knowledge is important for the development of therapeutic approaches to control SARS-CoV-2 infection by permitting the screening for neutraliz...

Descripción completa

Detalles Bibliográficos
Autores principales:	Condor Capcha, Jose Manuel, Lambert, Guerline, Dykxhoorn, Derek M., Salerno, Alessandro G., Hare, Joshua M., Whitt, Michael A., Pahwa, Savita, Jayaweera, Dushyantha T., Shehadeh, Lina A.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Frontiers Media S.A. 2021
Materias:	Cardiovascular Medicine
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7843445/ https://www.ncbi.nlm.nih.gov/pubmed/33521067 http://dx.doi.org/10.3389/fcvm.2020.618651

Ejemplares similares

Col4a3(-/-) Mice on Balb/C Background Have Less Severe Cardiorespiratory Phenotype and SGLT2 Over-Expression Compared to 129x1/SvJ and C57Bl/6 Backgrounds
por: Irion, Camila I., et al.
Publicado: (2022)

Peptide-Functionalized Dendrimer Nanocarriers for Targeted Microdystrophin Gene Delivery
por: Hersh, Jessica, et al.
Publicado: (2021)

Characterization of the spike protein of human coronavirus NL63 in receptor binding and pseudotype virus entry
por: Lin, Han-Xin, et al.
Publicado: (2011)

Pseudotyped Vesicular Stomatitis Virus for Analysis of Virus Entry Mediated by SARS Coronavirus Spike Proteins
por: Fukushi, Shuetsu, et al.
Publicado: (2007)

Mouse Model of Heart Failure With Preserved Ejection Fraction Driven by Hyperlipidemia and Enhanced Cardiac Low‐Density Lipoprotein Receptor Expression
por: Williams, Monique, et al.
Publicado: (2022)

Neutralization of SARS-CoV-2 spike pseudotyped virus by recombinant ACE2-Ig
por: Lei, Changhai, et al.
Publicado: (2020)

Protocol and Reagents for Pseudotyping Lentiviral Particles with SARS-CoV-2 Spike Protein for Neutralization Assays
por: Crawford, Katharine H. D., et al.
Publicado: (2020)

Development and effectiveness of pseudotyped SARS-CoV-2 system as determined by neutralizing efficiency and entry inhibition test in vitro
por: Yang, Ren, et al.
Publicado: (2020)

Methylene Blue Is a Nonspecific Protein–Protein Interaction Inhibitor with Potential for Repurposing as an Antiviral for COVID-19
por: Chuang, Sung-Ting, et al.
Publicado: (2022)

Optimized Pseudotyping Conditions for the SARS-COV-2 Spike Glycoprotein
por: Johnson, Marc C., et al.
Publicado: (2020)

Neutralization Assay with SARS-CoV-1 and SARS-CoV-2 Spike Pseudotyped Murine Leukemia Virions
por: Zheng, Yue, et al.
Publicado: (2020)

Neutralization assay with SARS-CoV-1 and SARS-CoV-2 spike pseudotyped murine leukemia virions
por: Zheng, Yue, et al.
Publicado: (2021)

Human Surfactant Protein D Binds Spike Protein and Acts as an Entry Inhibitor of SARS-CoV-2 Pseudotyped Viral Particles
por: Hsieh, Miao-Hsi, et al.
Publicado: (2021)

Longitudinally Profiling Neutralizing Antibody Response to SARS Coronavirus with Pseudotypes
por: Temperton, Nigel J., et al.
Publicado: (2005)

Pseudotype-Based Neutralization Assays for Influenza: A Systematic Analysis
por: Carnell, George William, et al.
Publicado: (2015)

Reliability of Pseudotyped Influenza Viral Particles in Neutralizing Antibody Detection
por: Yang, Jinghui, et al.
Publicado: (2014)

Chimeric influenza haemagglutinins: Generation and use in pseudotype neutralization assays
por: Ferrara, Francesca, et al.
Publicado: (2016)

Pseudotype Neutralization Assays: From Laboratory Bench to Data Analysis
por: Ferrara, Francesca, et al.
Publicado: (2018)

Examination of seroprevalence of coronavirus HKU1 infection with S protein-based ELISA and neutralization assay against viral spike pseudotyped virus
por: Chan, C.M., et al.
Publicado: (2009)

Assessment of HIV-1 entry inhibitors by MLV/HIV-1 pseudotyped vectors
por: Siegert, Sandra, et al.
Publicado: (2005)

Analyses of Entry Mechanisms of Novel Emerging Viruses Using Pseudotype VSV System
por: Tani, Hideki
Publicado: (2014)

The role of pseudotype neutralization assays in understanding SARS CoV-2
por: Cantoni, Diego, et al.
Publicado: (2021)

Nuclear Osteopontin Is a Marker of Advanced Heart Failure and Cardiac Allograft Vasculopathy: Evidence From Transplant and Retransplant Hearts
por: Irion, Camila Iansen, et al.
Publicado: (2020)

Comparison of JEV neutralization assay using pseudotyped JEV with the conventional plaque-reduction neutralization test
por: Lee, Hee-Jung, et al.
Publicado: (2014)

947. Stroke demographics and risk factor profile in HIV infected individuals in Florida
por: Ayoade, Folusakin, et al.
Publicado: (2020)

An optimised method for the production of MERS-CoV spike expressing viral pseudotypes
por: Grehan, K., et al.
Publicado: (2015)

Pseudotyped Vesicular Stomatitis Virus for Functional Analysis of Sars Coronavirus Spike Protein
por: Fukushi, Shuetsu, et al.
Publicado: (2006)

Quantification of Lyssavirus-Neutralizing Antibodies Using Vesicular Stomatitis Virus Pseudotype Particles
por: Moeschler, Sarah, et al.
Publicado: (2016)

N-Heterocyclic borneol derivatives as inhibitors of Marburg virus glycoprotein-mediated VSIV pseudotype entry
por: Kononova, A. A., et al.
Publicado: (2017)

Cytokine Effects on the Entry of Filovirus Envelope Pseudotyped Virus-Like Particles into Primary Human Macrophages
por: Stantchev, Tzanko S., et al.
Publicado: (2019)

Characterization of influenza A virus pseudotyped with the spike protein of porcine epidemic diarrhea virus
por: Wanitchang, Asawin, et al.
Publicado: (2018)

Human Immunodeficiency Viruses Pseudotyped with SARS-CoV-2 Spike Proteins Infect a Broad Spectrum of Human Cell Lines through Multiple Entry Mechanisms
por: Xu, Chuan, et al.
Publicado: (2021)

Human surfactant protein D facilitates SARS-CoV-2 pseudotype binding and entry in DC-SIGN expressing cells, and downregulates spike protein induced inflammation
por: Beirag, Nazar, et al.
Publicado: (2022)

HIV Promotes Atherosclerosis via Circulating Extracellular Vesicle MicroRNAs
por: Da Fonseca Ferreira, Andrea, et al.
Publicado: (2023)

Investigating antibody neutralization of lyssaviruses using lentiviral pseudotypes: a cross-species comparison
por: Wright, Edward, et al.
Publicado: (2008)

The Use of Hyperimmune Chicken Reference Sera Is Not Appropriate for the Validation of Influenza Pseudotype Neutralization Assays
por: Ferrara, Francesca, et al.
Publicado: (2017)

Development of a neutralization assay based on the pseudotyped chikungunya virus of a Korean isolate
por: Chung, Woo-Chang, et al.
Publicado: (2019)

Coronavirus Pseudotypes for All Circulating Human Coronaviruses for Quantification of Cross-Neutralizing Antibody Responses
por: Sampson, Alexander Thomas, et al.
Publicado: (2021)

A high throughput screening assay for inhibitors of SARS-CoV-2 pseudotyped particle entry
por: Xu, Miao, et al.
Publicado: (2021)

A high throughput screening assay for inhibitors of SARS-CoV-2 pseudotyped particle entry
por: Xu, Miao, et al.
Publicado: (2022)

Cannot write session to /tmp/vufind_sessions/sess_7ubu2vvsj5dqanm1p383l20e8k