Cargando…
Correlation of surface-enhanced Raman spectroscopic fingerprints of kidney transplant recipient urine with kidney function parameters
Routine monitoring of kidney transplant function is required for the standard care in post-transplantation management, including frequent measurements of serum creatinine with or without kidney biopsy. However, the invasiveness of these methods with potential for clinically significant complications...
Autores principales: | , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7843595/ https://www.ncbi.nlm.nih.gov/pubmed/33510308 http://dx.doi.org/10.1038/s41598-021-82113-7 |
_version_ | 1783644182155362304 |
---|---|
author | Huang, Zhongli Feng, Shijian Guan, Qiunong Lin, Tao Zhao, Jianhua Nguan, Christopher Y. C. Zeng, Haishan Harriman, David Li, Hong Du, Caigan |
author_facet | Huang, Zhongli Feng, Shijian Guan, Qiunong Lin, Tao Zhao, Jianhua Nguan, Christopher Y. C. Zeng, Haishan Harriman, David Li, Hong Du, Caigan |
author_sort | Huang, Zhongli |
collection | PubMed |
description | Routine monitoring of kidney transplant function is required for the standard care in post-transplantation management, including frequent measurements of serum creatinine with or without kidney biopsy. However, the invasiveness of these methods with potential for clinically significant complications makes them less than ideal. The objective of this study was to develop a non-invasive tool to monitor the kidney transplant function by using Surface-Enhanced Raman Spectroscopy (SERS). Urine and blood samples were collected from kidney transplant recipients after surgery. Silver nanoparticle-based SERS spectra of the urine were measured and evaluated using partial least squires (PLS) analysis. The SERS spectra were compared with conventional chemical markers of kidney transplant function to assess its predictive ability. A total of 110 kidney transplant recipients were included in this study. PLS results showed significant correlation with urine protein (R(2) = 0.4660, p < 0.01), creatinine (R(2) = 0.8106, p < 0.01), and urea (R(2) = 0.7808, p < 0.01). Furthermore, the prediction of the blood markers of kidney transplant function using the urine SERS spectra was indicated by R(2) = 0.7628 (p < 0.01) for serum creatinine and R(2) = 0.6539 (p < 0.01) for blood urea nitrogen. This preliminary study suggested that the urine SERS spectral analysis could be used as a convenient method for rapid assessment of kidney transplant function. |
format | Online Article Text |
id | pubmed-7843595 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-78435952021-01-29 Correlation of surface-enhanced Raman spectroscopic fingerprints of kidney transplant recipient urine with kidney function parameters Huang, Zhongli Feng, Shijian Guan, Qiunong Lin, Tao Zhao, Jianhua Nguan, Christopher Y. C. Zeng, Haishan Harriman, David Li, Hong Du, Caigan Sci Rep Article Routine monitoring of kidney transplant function is required for the standard care in post-transplantation management, including frequent measurements of serum creatinine with or without kidney biopsy. However, the invasiveness of these methods with potential for clinically significant complications makes them less than ideal. The objective of this study was to develop a non-invasive tool to monitor the kidney transplant function by using Surface-Enhanced Raman Spectroscopy (SERS). Urine and blood samples were collected from kidney transplant recipients after surgery. Silver nanoparticle-based SERS spectra of the urine were measured and evaluated using partial least squires (PLS) analysis. The SERS spectra were compared with conventional chemical markers of kidney transplant function to assess its predictive ability. A total of 110 kidney transplant recipients were included in this study. PLS results showed significant correlation with urine protein (R(2) = 0.4660, p < 0.01), creatinine (R(2) = 0.8106, p < 0.01), and urea (R(2) = 0.7808, p < 0.01). Furthermore, the prediction of the blood markers of kidney transplant function using the urine SERS spectra was indicated by R(2) = 0.7628 (p < 0.01) for serum creatinine and R(2) = 0.6539 (p < 0.01) for blood urea nitrogen. This preliminary study suggested that the urine SERS spectral analysis could be used as a convenient method for rapid assessment of kidney transplant function. Nature Publishing Group UK 2021-01-28 /pmc/articles/PMC7843595/ /pubmed/33510308 http://dx.doi.org/10.1038/s41598-021-82113-7 Text en © The Author(s) 2021 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Huang, Zhongli Feng, Shijian Guan, Qiunong Lin, Tao Zhao, Jianhua Nguan, Christopher Y. C. Zeng, Haishan Harriman, David Li, Hong Du, Caigan Correlation of surface-enhanced Raman spectroscopic fingerprints of kidney transplant recipient urine with kidney function parameters |
title | Correlation of surface-enhanced Raman spectroscopic fingerprints of kidney transplant recipient urine with kidney function parameters |
title_full | Correlation of surface-enhanced Raman spectroscopic fingerprints of kidney transplant recipient urine with kidney function parameters |
title_fullStr | Correlation of surface-enhanced Raman spectroscopic fingerprints of kidney transplant recipient urine with kidney function parameters |
title_full_unstemmed | Correlation of surface-enhanced Raman spectroscopic fingerprints of kidney transplant recipient urine with kidney function parameters |
title_short | Correlation of surface-enhanced Raman spectroscopic fingerprints of kidney transplant recipient urine with kidney function parameters |
title_sort | correlation of surface-enhanced raman spectroscopic fingerprints of kidney transplant recipient urine with kidney function parameters |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7843595/ https://www.ncbi.nlm.nih.gov/pubmed/33510308 http://dx.doi.org/10.1038/s41598-021-82113-7 |
work_keys_str_mv | AT huangzhongli correlationofsurfaceenhancedramanspectroscopicfingerprintsofkidneytransplantrecipienturinewithkidneyfunctionparameters AT fengshijian correlationofsurfaceenhancedramanspectroscopicfingerprintsofkidneytransplantrecipienturinewithkidneyfunctionparameters AT guanqiunong correlationofsurfaceenhancedramanspectroscopicfingerprintsofkidneytransplantrecipienturinewithkidneyfunctionparameters AT lintao correlationofsurfaceenhancedramanspectroscopicfingerprintsofkidneytransplantrecipienturinewithkidneyfunctionparameters AT zhaojianhua correlationofsurfaceenhancedramanspectroscopicfingerprintsofkidneytransplantrecipienturinewithkidneyfunctionparameters AT nguanchristopheryc correlationofsurfaceenhancedramanspectroscopicfingerprintsofkidneytransplantrecipienturinewithkidneyfunctionparameters AT zenghaishan correlationofsurfaceenhancedramanspectroscopicfingerprintsofkidneytransplantrecipienturinewithkidneyfunctionparameters AT harrimandavid correlationofsurfaceenhancedramanspectroscopicfingerprintsofkidneytransplantrecipienturinewithkidneyfunctionparameters AT lihong correlationofsurfaceenhancedramanspectroscopicfingerprintsofkidneytransplantrecipienturinewithkidneyfunctionparameters AT ducaigan correlationofsurfaceenhancedramanspectroscopicfingerprintsofkidneytransplantrecipienturinewithkidneyfunctionparameters |