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Characterization of the Complete Nucleotide Sequences of mcr-1-Encoding Plasmids From Enterobacterales Isolates in Retailed Raw Meat Products From the Czech Republic

The aim of our study was to determine complete nucleotide sequence of mcr-1-carrying plasmids from Enterobacterales isolates recovered from domestic and imported raw retailed meat and compare them with plasmids available at the GenBank sequence database. A set of 16 plasmids originating from Escheri...

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Autores principales: Zelendova, Marketa, Papagiannitsis, Costas C., Valcek, Adam, Medvecky, Matej, Bitar, Ibrahim, Hrabak, Jaroslav, Gelbicova, Tereza, Barakova, Alzbeta, Kutilova, Iva, Karpiskova, Renata, Dolejska, Monika
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7843963/
https://www.ncbi.nlm.nih.gov/pubmed/33519748
http://dx.doi.org/10.3389/fmicb.2020.604067
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author Zelendova, Marketa
Papagiannitsis, Costas C.
Valcek, Adam
Medvecky, Matej
Bitar, Ibrahim
Hrabak, Jaroslav
Gelbicova, Tereza
Barakova, Alzbeta
Kutilova, Iva
Karpiskova, Renata
Dolejska, Monika
author_facet Zelendova, Marketa
Papagiannitsis, Costas C.
Valcek, Adam
Medvecky, Matej
Bitar, Ibrahim
Hrabak, Jaroslav
Gelbicova, Tereza
Barakova, Alzbeta
Kutilova, Iva
Karpiskova, Renata
Dolejska, Monika
author_sort Zelendova, Marketa
collection PubMed
description The aim of our study was to determine complete nucleotide sequence of mcr-1-carrying plasmids from Enterobacterales isolates recovered from domestic and imported raw retailed meat and compare them with plasmids available at the GenBank sequence database. A set of 16 plasmids originating from Escherichia coli (n = 13), Klebsiella pneumoniae (n = 2), and Citrobacter braakii (n = 1) were analyzed. In our previous study, data from whole genome sequencing showed that mcr-1 gene was located on plasmids of different incompatibility groups (IncHI2, IncI2, and IncX4). The IncI2 (n = 3) and IncX4 (n = 8) plasmids harbored mcr-1.1 gene only, whereas IncHI2 sequence type 4 plasmids (n = 5) carried large multidrug resistance (MDR) regions. MDR regions of IncHI2 plasmids included additional antimicrobial resistance genes conferring resistance to β-lactams (bla(TEM−1)), aminoglycosides [aadA1, aadA2, and aph(6)-Id], macrolides [mef (B)], tetracycline (tetA, tetR), and sulphonamides (sul1, sul2, and sul3). Likewise, IncHI2 plasmids carried several insertion sequences including IS1, IS3, IS26, IS1326, and ISApl1. In conclusion, our findings confirmed the involvement of IncX4, IncI2, and IncHI2 plasmids in the dissemination of mcr-1.1 gene in several environmental niches, as in samples of retail meat originating from different geographical regions. In contrast to IncX4 and IncI2, IncHI2 plasmids were more diverse and carried additional genes for resistance to heavy metals and multiple antimicrobials.
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spelling pubmed-78439632021-01-30 Characterization of the Complete Nucleotide Sequences of mcr-1-Encoding Plasmids From Enterobacterales Isolates in Retailed Raw Meat Products From the Czech Republic Zelendova, Marketa Papagiannitsis, Costas C. Valcek, Adam Medvecky, Matej Bitar, Ibrahim Hrabak, Jaroslav Gelbicova, Tereza Barakova, Alzbeta Kutilova, Iva Karpiskova, Renata Dolejska, Monika Front Microbiol Microbiology The aim of our study was to determine complete nucleotide sequence of mcr-1-carrying plasmids from Enterobacterales isolates recovered from domestic and imported raw retailed meat and compare them with plasmids available at the GenBank sequence database. A set of 16 plasmids originating from Escherichia coli (n = 13), Klebsiella pneumoniae (n = 2), and Citrobacter braakii (n = 1) were analyzed. In our previous study, data from whole genome sequencing showed that mcr-1 gene was located on plasmids of different incompatibility groups (IncHI2, IncI2, and IncX4). The IncI2 (n = 3) and IncX4 (n = 8) plasmids harbored mcr-1.1 gene only, whereas IncHI2 sequence type 4 plasmids (n = 5) carried large multidrug resistance (MDR) regions. MDR regions of IncHI2 plasmids included additional antimicrobial resistance genes conferring resistance to β-lactams (bla(TEM−1)), aminoglycosides [aadA1, aadA2, and aph(6)-Id], macrolides [mef (B)], tetracycline (tetA, tetR), and sulphonamides (sul1, sul2, and sul3). Likewise, IncHI2 plasmids carried several insertion sequences including IS1, IS3, IS26, IS1326, and ISApl1. In conclusion, our findings confirmed the involvement of IncX4, IncI2, and IncHI2 plasmids in the dissemination of mcr-1.1 gene in several environmental niches, as in samples of retail meat originating from different geographical regions. In contrast to IncX4 and IncI2, IncHI2 plasmids were more diverse and carried additional genes for resistance to heavy metals and multiple antimicrobials. Frontiers Media S.A. 2021-01-15 /pmc/articles/PMC7843963/ /pubmed/33519748 http://dx.doi.org/10.3389/fmicb.2020.604067 Text en Copyright © 2021 Zelendova, Papagiannitsis, Valcek, Medvecky, Bitar, Hrabak, Gelbicova, Barakova, Kutilova, Karpiskova and Dolejska. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Zelendova, Marketa
Papagiannitsis, Costas C.
Valcek, Adam
Medvecky, Matej
Bitar, Ibrahim
Hrabak, Jaroslav
Gelbicova, Tereza
Barakova, Alzbeta
Kutilova, Iva
Karpiskova, Renata
Dolejska, Monika
Characterization of the Complete Nucleotide Sequences of mcr-1-Encoding Plasmids From Enterobacterales Isolates in Retailed Raw Meat Products From the Czech Republic
title Characterization of the Complete Nucleotide Sequences of mcr-1-Encoding Plasmids From Enterobacterales Isolates in Retailed Raw Meat Products From the Czech Republic
title_full Characterization of the Complete Nucleotide Sequences of mcr-1-Encoding Plasmids From Enterobacterales Isolates in Retailed Raw Meat Products From the Czech Republic
title_fullStr Characterization of the Complete Nucleotide Sequences of mcr-1-Encoding Plasmids From Enterobacterales Isolates in Retailed Raw Meat Products From the Czech Republic
title_full_unstemmed Characterization of the Complete Nucleotide Sequences of mcr-1-Encoding Plasmids From Enterobacterales Isolates in Retailed Raw Meat Products From the Czech Republic
title_short Characterization of the Complete Nucleotide Sequences of mcr-1-Encoding Plasmids From Enterobacterales Isolates in Retailed Raw Meat Products From the Czech Republic
title_sort characterization of the complete nucleotide sequences of mcr-1-encoding plasmids from enterobacterales isolates in retailed raw meat products from the czech republic
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7843963/
https://www.ncbi.nlm.nih.gov/pubmed/33519748
http://dx.doi.org/10.3389/fmicb.2020.604067
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