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Multiplexed CRISPR-Cas9 system in a single adeno-associated virus to simultaneously knock out redundant clock genes

The mammalian molecular clock is based on a transcription-translation feedback loop (TTFL) comprising the Period1, 2 (Per1, 2), Cryptochrome1, 2 (Cry1, 2), and Brain and Muscle ARNT-Like 1 (Bmal1) genes. The robustness of the TTFL is attributed to genetic redundancy among some essential clock genes,...

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Detalles Bibliográficos
Autores principales:	Kim, Boil, Kim, Jihoon, Chun, Minjeong, Park, Inah, Kwak, Damhyeon, Choi, Mijung, Kim, Kyungjin, Choe, Han Kyoung
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Nature Publishing Group UK 2021
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7844015/ https://www.ncbi.nlm.nih.gov/pubmed/33510438 http://dx.doi.org/10.1038/s41598-021-82287-0

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