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Dynamics of genome architecture and chromatin function during human B cell differentiation and neoplastic transformation
To investigate the three-dimensional (3D) genome architecture across normal B cell differentiation and in neoplastic cells from different subtypes of chronic lymphocytic leukemia and mantle cell lymphoma patients, here we integrate in situ Hi-C and nine additional omics layers. Beyond conventional a...
Autores principales: | , , , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7844026/ https://www.ncbi.nlm.nih.gov/pubmed/33510161 http://dx.doi.org/10.1038/s41467-020-20849-y |
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author | Vilarrasa-Blasi, Roser Soler-Vila, Paula Verdaguer-Dot, Núria Russiñol, Núria Di Stefano, Marco Chapaprieta, Vicente Clot, Guillem Farabella, Irene Cuscó, Pol Kulis, Marta Agirre, Xabier Prosper, Felipe Beekman, Renée Beà, Silvia Colomer, Dolors Stunnenberg, Hendrik G. Gut, Ivo Campo, Elias Marti-Renom, Marc A. Martin-Subero, José Ignacio |
author_facet | Vilarrasa-Blasi, Roser Soler-Vila, Paula Verdaguer-Dot, Núria Russiñol, Núria Di Stefano, Marco Chapaprieta, Vicente Clot, Guillem Farabella, Irene Cuscó, Pol Kulis, Marta Agirre, Xabier Prosper, Felipe Beekman, Renée Beà, Silvia Colomer, Dolors Stunnenberg, Hendrik G. Gut, Ivo Campo, Elias Marti-Renom, Marc A. Martin-Subero, José Ignacio |
author_sort | Vilarrasa-Blasi, Roser |
collection | PubMed |
description | To investigate the three-dimensional (3D) genome architecture across normal B cell differentiation and in neoplastic cells from different subtypes of chronic lymphocytic leukemia and mantle cell lymphoma patients, here we integrate in situ Hi-C and nine additional omics layers. Beyond conventional active (A) and inactive (B) compartments, we uncover a highly-dynamic intermediate compartment enriched in poised and polycomb-repressed chromatin. During B cell development, 28% of the compartments change, mostly involving a widespread chromatin activation from naive to germinal center B cells and a reversal to the naive state upon further maturation into memory B cells. B cell neoplasms are characterized by both entity and subtype-specific alterations in 3D genome organization, including large chromatin blocks spanning key disease-specific genes. This study indicates that 3D genome interactions are extensively modulated during normal B cell differentiation and that the genome of B cell neoplasias acquires a tumor-specific 3D genome architecture. |
format | Online Article Text |
id | pubmed-7844026 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-78440262021-02-08 Dynamics of genome architecture and chromatin function during human B cell differentiation and neoplastic transformation Vilarrasa-Blasi, Roser Soler-Vila, Paula Verdaguer-Dot, Núria Russiñol, Núria Di Stefano, Marco Chapaprieta, Vicente Clot, Guillem Farabella, Irene Cuscó, Pol Kulis, Marta Agirre, Xabier Prosper, Felipe Beekman, Renée Beà, Silvia Colomer, Dolors Stunnenberg, Hendrik G. Gut, Ivo Campo, Elias Marti-Renom, Marc A. Martin-Subero, José Ignacio Nat Commun Article To investigate the three-dimensional (3D) genome architecture across normal B cell differentiation and in neoplastic cells from different subtypes of chronic lymphocytic leukemia and mantle cell lymphoma patients, here we integrate in situ Hi-C and nine additional omics layers. Beyond conventional active (A) and inactive (B) compartments, we uncover a highly-dynamic intermediate compartment enriched in poised and polycomb-repressed chromatin. During B cell development, 28% of the compartments change, mostly involving a widespread chromatin activation from naive to germinal center B cells and a reversal to the naive state upon further maturation into memory B cells. B cell neoplasms are characterized by both entity and subtype-specific alterations in 3D genome organization, including large chromatin blocks spanning key disease-specific genes. This study indicates that 3D genome interactions are extensively modulated during normal B cell differentiation and that the genome of B cell neoplasias acquires a tumor-specific 3D genome architecture. Nature Publishing Group UK 2021-01-28 /pmc/articles/PMC7844026/ /pubmed/33510161 http://dx.doi.org/10.1038/s41467-020-20849-y Text en © The Author(s) 2021 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Vilarrasa-Blasi, Roser Soler-Vila, Paula Verdaguer-Dot, Núria Russiñol, Núria Di Stefano, Marco Chapaprieta, Vicente Clot, Guillem Farabella, Irene Cuscó, Pol Kulis, Marta Agirre, Xabier Prosper, Felipe Beekman, Renée Beà, Silvia Colomer, Dolors Stunnenberg, Hendrik G. Gut, Ivo Campo, Elias Marti-Renom, Marc A. Martin-Subero, José Ignacio Dynamics of genome architecture and chromatin function during human B cell differentiation and neoplastic transformation |
title | Dynamics of genome architecture and chromatin function during human B cell differentiation and neoplastic transformation |
title_full | Dynamics of genome architecture and chromatin function during human B cell differentiation and neoplastic transformation |
title_fullStr | Dynamics of genome architecture and chromatin function during human B cell differentiation and neoplastic transformation |
title_full_unstemmed | Dynamics of genome architecture and chromatin function during human B cell differentiation and neoplastic transformation |
title_short | Dynamics of genome architecture and chromatin function during human B cell differentiation and neoplastic transformation |
title_sort | dynamics of genome architecture and chromatin function during human b cell differentiation and neoplastic transformation |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7844026/ https://www.ncbi.nlm.nih.gov/pubmed/33510161 http://dx.doi.org/10.1038/s41467-020-20849-y |
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