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A Longitudinal Analysis Reveals Early Activation and Late Alterations in B Cells During Primary HIV Infection in Mozambican Adults

Primary HIV infection (PHI) and subsequent chronic infection alter B-cell compartment. However, longitudinal analysis defining the dynamics of B-cell alterations are still limited. We longitudinally studied B-cell subsets in individuals followed for 1 year after PHI (n = 40). Treated and untreated c...

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Autores principales: Jiménez, Montse, Pastor, Lucía, Urrea, Victor, Rodríguez de la Concepción, María Luisa, Parker, Erica, Fuente-Soro, Laura, Jairoce, Chenjerai, Mandomando, Inacio, Carrillo, Jorge, Naniche, Denise, Blanco, Julià
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7844141/
https://www.ncbi.nlm.nih.gov/pubmed/33519823
http://dx.doi.org/10.3389/fimmu.2020.614319
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author Jiménez, Montse
Pastor, Lucía
Urrea, Victor
Rodríguez de la Concepción, María Luisa
Parker, Erica
Fuente-Soro, Laura
Jairoce, Chenjerai
Mandomando, Inacio
Carrillo, Jorge
Naniche, Denise
Blanco, Julià
author_facet Jiménez, Montse
Pastor, Lucía
Urrea, Victor
Rodríguez de la Concepción, María Luisa
Parker, Erica
Fuente-Soro, Laura
Jairoce, Chenjerai
Mandomando, Inacio
Carrillo, Jorge
Naniche, Denise
Blanco, Julià
author_sort Jiménez, Montse
collection PubMed
description Primary HIV infection (PHI) and subsequent chronic infection alter B-cell compartment. However, longitudinal analysis defining the dynamics of B-cell alterations are still limited. We longitudinally studied B-cell subsets in individuals followed for 1 year after PHI (n = 40). Treated and untreated chronic HIV infected (n = 56) and HIV-uninfected individuals (n = 58) were recruited as reference groups at the Manhiça District in Mozambique. B cells were analyzed by multicolor flow-cytometry. Anti-HIV humoral response and plasma cytokines were assessed by ELISA or Luminex-based technology. A generalized activation of B cells induced by HIV occurs early after infection and is characterized by increases in Activated and Tissue-like memory cells, decreases in IgM-IgD- (switched) and IgM-only B cells. These alterations remain mostly stable until chronic infection and are reverted in part by ART. In contrast, other parameters followed particular dynamics: PD-1 expression in memory cells decreases progressively during the first year of infection, Transitional B cells expand at month 3–4 after infection, and Marginal zone-like B cells show a late depletion. Plasmablasts expand 2 months after infection linked to plasma viral load and anti-p24 IgG3 responses. Most of well-defined changes induced by HIV in B-cell activation and memory subsets are readily observed after PHI, lasting until ART initiation. However, subsequent changes occur after sustained viral infection. These data indicate that HIV infection impacts B cells in several waves over time, and highlight that early treatment would result in beneficial effects on the B-cell compartment.
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spelling pubmed-78441412021-01-30 A Longitudinal Analysis Reveals Early Activation and Late Alterations in B Cells During Primary HIV Infection in Mozambican Adults Jiménez, Montse Pastor, Lucía Urrea, Victor Rodríguez de la Concepción, María Luisa Parker, Erica Fuente-Soro, Laura Jairoce, Chenjerai Mandomando, Inacio Carrillo, Jorge Naniche, Denise Blanco, Julià Front Immunol Immunology Primary HIV infection (PHI) and subsequent chronic infection alter B-cell compartment. However, longitudinal analysis defining the dynamics of B-cell alterations are still limited. We longitudinally studied B-cell subsets in individuals followed for 1 year after PHI (n = 40). Treated and untreated chronic HIV infected (n = 56) and HIV-uninfected individuals (n = 58) were recruited as reference groups at the Manhiça District in Mozambique. B cells were analyzed by multicolor flow-cytometry. Anti-HIV humoral response and plasma cytokines were assessed by ELISA or Luminex-based technology. A generalized activation of B cells induced by HIV occurs early after infection and is characterized by increases in Activated and Tissue-like memory cells, decreases in IgM-IgD- (switched) and IgM-only B cells. These alterations remain mostly stable until chronic infection and are reverted in part by ART. In contrast, other parameters followed particular dynamics: PD-1 expression in memory cells decreases progressively during the first year of infection, Transitional B cells expand at month 3–4 after infection, and Marginal zone-like B cells show a late depletion. Plasmablasts expand 2 months after infection linked to plasma viral load and anti-p24 IgG3 responses. Most of well-defined changes induced by HIV in B-cell activation and memory subsets are readily observed after PHI, lasting until ART initiation. However, subsequent changes occur after sustained viral infection. These data indicate that HIV infection impacts B cells in several waves over time, and highlight that early treatment would result in beneficial effects on the B-cell compartment. Frontiers Media S.A. 2021-01-15 /pmc/articles/PMC7844141/ /pubmed/33519823 http://dx.doi.org/10.3389/fimmu.2020.614319 Text en Copyright © 2021 Jiménez, Pastor, Urrea, Rodríguez de la Concepción, Parker, Fuente-Soro, Jairoce, Mandomando, Carrillo, Naniche and Blanco http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Immunology
Jiménez, Montse
Pastor, Lucía
Urrea, Victor
Rodríguez de la Concepción, María Luisa
Parker, Erica
Fuente-Soro, Laura
Jairoce, Chenjerai
Mandomando, Inacio
Carrillo, Jorge
Naniche, Denise
Blanco, Julià
A Longitudinal Analysis Reveals Early Activation and Late Alterations in B Cells During Primary HIV Infection in Mozambican Adults
title A Longitudinal Analysis Reveals Early Activation and Late Alterations in B Cells During Primary HIV Infection in Mozambican Adults
title_full A Longitudinal Analysis Reveals Early Activation and Late Alterations in B Cells During Primary HIV Infection in Mozambican Adults
title_fullStr A Longitudinal Analysis Reveals Early Activation and Late Alterations in B Cells During Primary HIV Infection in Mozambican Adults
title_full_unstemmed A Longitudinal Analysis Reveals Early Activation and Late Alterations in B Cells During Primary HIV Infection in Mozambican Adults
title_short A Longitudinal Analysis Reveals Early Activation and Late Alterations in B Cells During Primary HIV Infection in Mozambican Adults
title_sort longitudinal analysis reveals early activation and late alterations in b cells during primary hiv infection in mozambican adults
topic Immunology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7844141/
https://www.ncbi.nlm.nih.gov/pubmed/33519823
http://dx.doi.org/10.3389/fimmu.2020.614319
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