Cargando…
Impact of seed color and storage time on the radish seed germination and sprout growth in plasma agriculture
The use of low-temperature plasma for the pre-sowing seed treatment is still in the early stage of research; thus, numerous factors affecting germination percentage, seedling growth, and yield remains unknown. This study aimed to estimate how two critical factors, such as harvest year and seed coat...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7844220/ https://www.ncbi.nlm.nih.gov/pubmed/33510231 http://dx.doi.org/10.1038/s41598-021-81175-x |
_version_ | 1783644299011817472 |
---|---|
author | Attri, Pankaj Ishikawa, Kenji Okumura, Takamasa Koga, Kazunori Shiratani, Masaharu Mildaziene, Vida |
author_facet | Attri, Pankaj Ishikawa, Kenji Okumura, Takamasa Koga, Kazunori Shiratani, Masaharu Mildaziene, Vida |
author_sort | Attri, Pankaj |
collection | PubMed |
description | The use of low-temperature plasma for the pre-sowing seed treatment is still in the early stage of research; thus, numerous factors affecting germination percentage, seedling growth, and yield remains unknown. This study aimed to estimate how two critical factors, such as harvest year and seed coat color, affect the percentage of germination and seedling growth after plasma treatment. Radish seeds stored for 2 and 1 year after harvesting (harvested in 2017 and 2018) were sorted into two colors (brown and grey) to investigate the plasma effect on harvest year and seed coat color. We analyzed the amounts of seed phytohormones and antioxidant (γ-tocopherol) were analyzed using mass spectrometry, and physical changes were studied using SEM, EDX, and EPR to understand the mechanism of plasma-induced changes in radish seeds. The obtained results revealed that plasma treatment on seeds affects the germination kinetics, and the maximal germination percentage depends on seed color and the time of seed storage after harvest. Through this study, for the first time, we demonstrated that physical and chemical changes in radish seeds after plasma treatment depends upon the seed color and harvest year. Positive effects of plasma treatment on growth are stronger for sprouts from seeds harvested in 2017 than in 2018. The plasma treatment effect on the sprouts germinated from grey seeds effect was stronger than sprouts from brown radish seeds. The amounts of gibberellin A3 and abscisic acid in control seeds strongly depended on the seed color, and plasma induced changes were better in grey seeds harvested in 2017. Therefore, this study reveals that Air scalar-DBD plasma's reactive oxygen and nitrogen species (RONS) can efficiently accelerate germination and growth in older seeds. |
format | Online Article Text |
id | pubmed-7844220 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-78442202021-02-01 Impact of seed color and storage time on the radish seed germination and sprout growth in plasma agriculture Attri, Pankaj Ishikawa, Kenji Okumura, Takamasa Koga, Kazunori Shiratani, Masaharu Mildaziene, Vida Sci Rep Article The use of low-temperature plasma for the pre-sowing seed treatment is still in the early stage of research; thus, numerous factors affecting germination percentage, seedling growth, and yield remains unknown. This study aimed to estimate how two critical factors, such as harvest year and seed coat color, affect the percentage of germination and seedling growth after plasma treatment. Radish seeds stored for 2 and 1 year after harvesting (harvested in 2017 and 2018) were sorted into two colors (brown and grey) to investigate the plasma effect on harvest year and seed coat color. We analyzed the amounts of seed phytohormones and antioxidant (γ-tocopherol) were analyzed using mass spectrometry, and physical changes were studied using SEM, EDX, and EPR to understand the mechanism of plasma-induced changes in radish seeds. The obtained results revealed that plasma treatment on seeds affects the germination kinetics, and the maximal germination percentage depends on seed color and the time of seed storage after harvest. Through this study, for the first time, we demonstrated that physical and chemical changes in radish seeds after plasma treatment depends upon the seed color and harvest year. Positive effects of plasma treatment on growth are stronger for sprouts from seeds harvested in 2017 than in 2018. The plasma treatment effect on the sprouts germinated from grey seeds effect was stronger than sprouts from brown radish seeds. The amounts of gibberellin A3 and abscisic acid in control seeds strongly depended on the seed color, and plasma induced changes were better in grey seeds harvested in 2017. Therefore, this study reveals that Air scalar-DBD plasma's reactive oxygen and nitrogen species (RONS) can efficiently accelerate germination and growth in older seeds. Nature Publishing Group UK 2021-01-28 /pmc/articles/PMC7844220/ /pubmed/33510231 http://dx.doi.org/10.1038/s41598-021-81175-x Text en © The Author(s) 2021 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Attri, Pankaj Ishikawa, Kenji Okumura, Takamasa Koga, Kazunori Shiratani, Masaharu Mildaziene, Vida Impact of seed color and storage time on the radish seed germination and sprout growth in plasma agriculture |
title | Impact of seed color and storage time on the radish seed germination and sprout growth in plasma agriculture |
title_full | Impact of seed color and storage time on the radish seed germination and sprout growth in plasma agriculture |
title_fullStr | Impact of seed color and storage time on the radish seed germination and sprout growth in plasma agriculture |
title_full_unstemmed | Impact of seed color and storage time on the radish seed germination and sprout growth in plasma agriculture |
title_short | Impact of seed color and storage time on the radish seed germination and sprout growth in plasma agriculture |
title_sort | impact of seed color and storage time on the radish seed germination and sprout growth in plasma agriculture |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7844220/ https://www.ncbi.nlm.nih.gov/pubmed/33510231 http://dx.doi.org/10.1038/s41598-021-81175-x |
work_keys_str_mv | AT attripankaj impactofseedcolorandstoragetimeontheradishseedgerminationandsproutgrowthinplasmaagriculture AT ishikawakenji impactofseedcolorandstoragetimeontheradishseedgerminationandsproutgrowthinplasmaagriculture AT okumuratakamasa impactofseedcolorandstoragetimeontheradishseedgerminationandsproutgrowthinplasmaagriculture AT kogakazunori impactofseedcolorandstoragetimeontheradishseedgerminationandsproutgrowthinplasmaagriculture AT shiratanimasaharu impactofseedcolorandstoragetimeontheradishseedgerminationandsproutgrowthinplasmaagriculture AT mildazienevida impactofseedcolorandstoragetimeontheradishseedgerminationandsproutgrowthinplasmaagriculture |