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Redirecting substrate regioselectivity using engineered ΔN(123)-GBD-CD2 branching sucrases for the production of pentasaccharide repeating units of S. flexneri 3a, 4a and 4b haptens
The (chemo-)enzymatic synthesis of oligosaccharides has been hampered by the lack of appropriate enzymatic tools with requisite regio- and stereo-specificities. Engineering of carbohydrate-active enzymes, in particular targeting the enzyme active site, has notably led to catalysts with altered regio...
Autores principales: | Benkoulouche, Mounir, Ben Imeddourene, Akli, Barel, Louis-Antoine, Le Heiget, Guillaume, Pizzut, Sandra, Kulyk, Hanna, Bellvert, Floriant, Bozonnet, Sophie, Mulard, Laurence A., Remaud-Siméon, Magali, Moulis, Claire, André, Isabelle |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7844235/ https://www.ncbi.nlm.nih.gov/pubmed/33510212 http://dx.doi.org/10.1038/s41598-021-81719-1 |
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