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Non pharmacological high-intensity ultrasound treatment of human dermal fibroblasts to accelerate wound healing

Inspired by the effectiveness of low-intensity ultrasound on tissue regeneration, we investigated the potential effect of short-term high-intensity ultrasound treatment for acceleration of wound healing in an in vitro wound model and dermal equivalent, both comprising human dermal fibroblasts. Short...

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Autores principales: Lee, Jeong Yu, Min, Dae-Jin, Kim, Wanil, Bin, Bum-Ho, Kim, Kyuhan, Cho, Eun-Gyung
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7844265/
https://www.ncbi.nlm.nih.gov/pubmed/33510199
http://dx.doi.org/10.1038/s41598-021-81878-1
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author Lee, Jeong Yu
Min, Dae-Jin
Kim, Wanil
Bin, Bum-Ho
Kim, Kyuhan
Cho, Eun-Gyung
author_facet Lee, Jeong Yu
Min, Dae-Jin
Kim, Wanil
Bin, Bum-Ho
Kim, Kyuhan
Cho, Eun-Gyung
author_sort Lee, Jeong Yu
collection PubMed
description Inspired by the effectiveness of low-intensity ultrasound on tissue regeneration, we investigated the potential effect of short-term high-intensity ultrasound treatment for acceleration of wound healing in an in vitro wound model and dermal equivalent, both comprising human dermal fibroblasts. Short-term ultrasound of various amplitudes significantly increased the proliferation and migration of fibroblasts and subsequently increased the production of the extracellular matrix components fibronectin and collagen type I, both of which are important for wound healing and are secreted by fibroblasts. In addition, ultrasound treatment increased the contraction of a fibroblast-embedded three-dimensional collagen matrix, and the effect was synergistically increased in the presence of TGF-β. RNA-sequencing and bioinformatics analyses revealed changes in gene expression and p38 and ERK1/2 MAPK pathway activation in the ultrasound-stimulated fibroblasts. Our findings suggest that ultrasound as a mechanical stimulus can activate human dermal fibroblasts. Therefore, the activation of fibroblasts using ultrasound may improve the healing of various types of wounds and increase skin regeneration.
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spelling pubmed-78442652021-02-01 Non pharmacological high-intensity ultrasound treatment of human dermal fibroblasts to accelerate wound healing Lee, Jeong Yu Min, Dae-Jin Kim, Wanil Bin, Bum-Ho Kim, Kyuhan Cho, Eun-Gyung Sci Rep Article Inspired by the effectiveness of low-intensity ultrasound on tissue regeneration, we investigated the potential effect of short-term high-intensity ultrasound treatment for acceleration of wound healing in an in vitro wound model and dermal equivalent, both comprising human dermal fibroblasts. Short-term ultrasound of various amplitudes significantly increased the proliferation and migration of fibroblasts and subsequently increased the production of the extracellular matrix components fibronectin and collagen type I, both of which are important for wound healing and are secreted by fibroblasts. In addition, ultrasound treatment increased the contraction of a fibroblast-embedded three-dimensional collagen matrix, and the effect was synergistically increased in the presence of TGF-β. RNA-sequencing and bioinformatics analyses revealed changes in gene expression and p38 and ERK1/2 MAPK pathway activation in the ultrasound-stimulated fibroblasts. Our findings suggest that ultrasound as a mechanical stimulus can activate human dermal fibroblasts. Therefore, the activation of fibroblasts using ultrasound may improve the healing of various types of wounds and increase skin regeneration. Nature Publishing Group UK 2021-01-28 /pmc/articles/PMC7844265/ /pubmed/33510199 http://dx.doi.org/10.1038/s41598-021-81878-1 Text en © The Author(s) 2021 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Lee, Jeong Yu
Min, Dae-Jin
Kim, Wanil
Bin, Bum-Ho
Kim, Kyuhan
Cho, Eun-Gyung
Non pharmacological high-intensity ultrasound treatment of human dermal fibroblasts to accelerate wound healing
title Non pharmacological high-intensity ultrasound treatment of human dermal fibroblasts to accelerate wound healing
title_full Non pharmacological high-intensity ultrasound treatment of human dermal fibroblasts to accelerate wound healing
title_fullStr Non pharmacological high-intensity ultrasound treatment of human dermal fibroblasts to accelerate wound healing
title_full_unstemmed Non pharmacological high-intensity ultrasound treatment of human dermal fibroblasts to accelerate wound healing
title_short Non pharmacological high-intensity ultrasound treatment of human dermal fibroblasts to accelerate wound healing
title_sort non pharmacological high-intensity ultrasound treatment of human dermal fibroblasts to accelerate wound healing
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7844265/
https://www.ncbi.nlm.nih.gov/pubmed/33510199
http://dx.doi.org/10.1038/s41598-021-81878-1
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