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Neuronal delivery of nanoparticles via nerve fibres in the skin
Accessing the peripheral nervous system (PNS) by topically applied nanoparticles is a simple and novel approach with clinical applications in several PNS disorders. Skin is richly innervated by long peripheral axons that arise from cell bodies located distally within ganglia. In this study we attemp...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7844288/ https://www.ncbi.nlm.nih.gov/pubmed/33510229 http://dx.doi.org/10.1038/s41598-021-81995-x |
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author | Katiyar, Neeraj Raju, Gayathri Madhusudanan, Pallavi Gopalakrishnan-Prema, Vignesh Shankarappa, Sahadev A. |
author_facet | Katiyar, Neeraj Raju, Gayathri Madhusudanan, Pallavi Gopalakrishnan-Prema, Vignesh Shankarappa, Sahadev A. |
author_sort | Katiyar, Neeraj |
collection | PubMed |
description | Accessing the peripheral nervous system (PNS) by topically applied nanoparticles is a simple and novel approach with clinical applications in several PNS disorders. Skin is richly innervated by long peripheral axons that arise from cell bodies located distally within ganglia. In this study we attempt to target dorsal root ganglia (DRG) neurons, via their axons by topical application of lectin-functionalized gold nanoparticles (IB4-AuNP). In vitro, 140.2 ± 1.9 nm IB4-AuNP were found to bind both axons and cell bodies of DRG neurons, and AuNP applied at the axonal terminals were found to translocate to the cell bodies. Topical application of IB4-AuNP on rat hind-paw resulted in accumulation of three to fourfold higher AuNP in lumbar DRG than in contralateral control DRGs. Results from this study clearly suggest that topically applied nanoparticles with neurotropic targeting ligands can be utilized for delivering nanoparticles to neuronal cell bodies via axonal transport mechanisms. |
format | Online Article Text |
id | pubmed-7844288 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-78442882021-02-01 Neuronal delivery of nanoparticles via nerve fibres in the skin Katiyar, Neeraj Raju, Gayathri Madhusudanan, Pallavi Gopalakrishnan-Prema, Vignesh Shankarappa, Sahadev A. Sci Rep Article Accessing the peripheral nervous system (PNS) by topically applied nanoparticles is a simple and novel approach with clinical applications in several PNS disorders. Skin is richly innervated by long peripheral axons that arise from cell bodies located distally within ganglia. In this study we attempt to target dorsal root ganglia (DRG) neurons, via their axons by topical application of lectin-functionalized gold nanoparticles (IB4-AuNP). In vitro, 140.2 ± 1.9 nm IB4-AuNP were found to bind both axons and cell bodies of DRG neurons, and AuNP applied at the axonal terminals were found to translocate to the cell bodies. Topical application of IB4-AuNP on rat hind-paw resulted in accumulation of three to fourfold higher AuNP in lumbar DRG than in contralateral control DRGs. Results from this study clearly suggest that topically applied nanoparticles with neurotropic targeting ligands can be utilized for delivering nanoparticles to neuronal cell bodies via axonal transport mechanisms. Nature Publishing Group UK 2021-01-28 /pmc/articles/PMC7844288/ /pubmed/33510229 http://dx.doi.org/10.1038/s41598-021-81995-x Text en © The Author(s) 2021 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Katiyar, Neeraj Raju, Gayathri Madhusudanan, Pallavi Gopalakrishnan-Prema, Vignesh Shankarappa, Sahadev A. Neuronal delivery of nanoparticles via nerve fibres in the skin |
title | Neuronal delivery of nanoparticles via nerve fibres in the skin |
title_full | Neuronal delivery of nanoparticles via nerve fibres in the skin |
title_fullStr | Neuronal delivery of nanoparticles via nerve fibres in the skin |
title_full_unstemmed | Neuronal delivery of nanoparticles via nerve fibres in the skin |
title_short | Neuronal delivery of nanoparticles via nerve fibres in the skin |
title_sort | neuronal delivery of nanoparticles via nerve fibres in the skin |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7844288/ https://www.ncbi.nlm.nih.gov/pubmed/33510229 http://dx.doi.org/10.1038/s41598-021-81995-x |
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