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Effect of mild α-chymotrypsin treatment of highly viscous semen samples on fertilization rates

BACKGROUND: Highly viscous semen reduces sperm motility significantly and can contribute to infertility. When processing semen samples, few techniques exist to induce liquefaction in case of seminal hyperviscosity such as different washing steps and mechanical treatment. The use of α-chymotrypsin se...

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Autores principales: Schallmoser, Andreas, Bakjaji, Fadi, Königsberger, Sebastian, John, Julia, Färber, Cara, Schmidt, Elke, Breitenbach-Koller, Hannelore, Allam, Jean-Pierre, Verguts, Jasper, Sänger, Nicole
Formato: Online Artículo Texto
Lenguaje:English
Publicado: AME Publishing Company 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7844487/
https://www.ncbi.nlm.nih.gov/pubmed/33532332
http://dx.doi.org/10.21037/tau-20-1013
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author Schallmoser, Andreas
Bakjaji, Fadi
Königsberger, Sebastian
John, Julia
Färber, Cara
Schmidt, Elke
Breitenbach-Koller, Hannelore
Allam, Jean-Pierre
Verguts, Jasper
Sänger, Nicole
author_facet Schallmoser, Andreas
Bakjaji, Fadi
Königsberger, Sebastian
John, Julia
Färber, Cara
Schmidt, Elke
Breitenbach-Koller, Hannelore
Allam, Jean-Pierre
Verguts, Jasper
Sänger, Nicole
author_sort Schallmoser, Andreas
collection PubMed
description BACKGROUND: Highly viscous semen reduces sperm motility significantly and can contribute to infertility. When processing semen samples, few techniques exist to induce liquefaction in case of seminal hyperviscosity such as different washing steps and mechanical treatment. The use of α-chymotrypsin seems controversial due to possible negative effects on fertilisation rates after in vitro fertilization (IVF). The main objective of this study was to examine the influence of mild α-chymotrypsin treatment of semen samples on the fertilisation rate after artificial reproductive treatment (ART). METHODS: The fertilization rate of 52 ART cycles was examined following IVF using a low dose of α-chymotrypsin to induce liquefaction of highly viscous semen and was compared to a control group of 88 ART cycles. RESULTS: There was no significant difference in the fertilization rates of α-chymotrypsin treated semen samples compared to the control group; pregnancy rates were unaffected. CONCLUSIONS: The use of mild α-chymotrypsin treatment of semen samples in case of hyperviscosity does not appear to impact negatively on the fertilization rates after ART and could be regarded as an additional method to induce liquefaction of highly viscous semen samples in IVF.
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spelling pubmed-78444872021-02-01 Effect of mild α-chymotrypsin treatment of highly viscous semen samples on fertilization rates Schallmoser, Andreas Bakjaji, Fadi Königsberger, Sebastian John, Julia Färber, Cara Schmidt, Elke Breitenbach-Koller, Hannelore Allam, Jean-Pierre Verguts, Jasper Sänger, Nicole Transl Androl Urol Original Article BACKGROUND: Highly viscous semen reduces sperm motility significantly and can contribute to infertility. When processing semen samples, few techniques exist to induce liquefaction in case of seminal hyperviscosity such as different washing steps and mechanical treatment. The use of α-chymotrypsin seems controversial due to possible negative effects on fertilisation rates after in vitro fertilization (IVF). The main objective of this study was to examine the influence of mild α-chymotrypsin treatment of semen samples on the fertilisation rate after artificial reproductive treatment (ART). METHODS: The fertilization rate of 52 ART cycles was examined following IVF using a low dose of α-chymotrypsin to induce liquefaction of highly viscous semen and was compared to a control group of 88 ART cycles. RESULTS: There was no significant difference in the fertilization rates of α-chymotrypsin treated semen samples compared to the control group; pregnancy rates were unaffected. CONCLUSIONS: The use of mild α-chymotrypsin treatment of semen samples in case of hyperviscosity does not appear to impact negatively on the fertilization rates after ART and could be regarded as an additional method to induce liquefaction of highly viscous semen samples in IVF. AME Publishing Company 2021-01 /pmc/articles/PMC7844487/ /pubmed/33532332 http://dx.doi.org/10.21037/tau-20-1013 Text en 2021 Translational Andrology and Urology. All rights reserved. https://creativecommons.org/licenses/by-nc-nd/4.0/Open Access Statement: This is an Open Access article distributed in accordance with the Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License (CC BY-NC-ND 4.0), which permits the non-commercial replication and distribution of the article with the strict proviso that no changes or edits are made and the original work is properly cited (including links to both the formal publication through the relevant DOI and the license). See: https://creativecommons.org/licenses/by-nc-nd/4.0 (https://creativecommons.org/licenses/by-nc-nd/4.0/) .
spellingShingle Original Article
Schallmoser, Andreas
Bakjaji, Fadi
Königsberger, Sebastian
John, Julia
Färber, Cara
Schmidt, Elke
Breitenbach-Koller, Hannelore
Allam, Jean-Pierre
Verguts, Jasper
Sänger, Nicole
Effect of mild α-chymotrypsin treatment of highly viscous semen samples on fertilization rates
title Effect of mild α-chymotrypsin treatment of highly viscous semen samples on fertilization rates
title_full Effect of mild α-chymotrypsin treatment of highly viscous semen samples on fertilization rates
title_fullStr Effect of mild α-chymotrypsin treatment of highly viscous semen samples on fertilization rates
title_full_unstemmed Effect of mild α-chymotrypsin treatment of highly viscous semen samples on fertilization rates
title_short Effect of mild α-chymotrypsin treatment of highly viscous semen samples on fertilization rates
title_sort effect of mild α-chymotrypsin treatment of highly viscous semen samples on fertilization rates
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7844487/
https://www.ncbi.nlm.nih.gov/pubmed/33532332
http://dx.doi.org/10.21037/tau-20-1013
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