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Cathepsin F Knockdown Induces Proliferation and Inhibits Apoptosis in Gastric Cancer Cells

Gastric cancer (GC) is one of the most common cancers in the world. The cathepsin F (CTSF) gene has recently been found to participate in the progression of several types of cancer. However, the clinical characteristics and function of CTSF in GC as well as its molecular mechanisms are not clear. Si...

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Autores principales: Ji, Ce, Zhao, Ying, Kou, You-Wei, Shao, Hua, Guo, Lin, Bao, Chen-Hui, Jiang, Ben-Chun, Chen, Xin-Ying, Dai, Jing-Wei, Tong, Yu-Xin, Yang, Ren, Sun, Wei, Wang, Qiang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cognizant Communication Corporation 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7844561/
https://www.ncbi.nlm.nih.gov/pubmed/28474574
http://dx.doi.org/10.3727/096504017X14928634401204
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author Ji, Ce
Zhao, Ying
Kou, You-Wei
Shao, Hua
Guo, Lin
Bao, Chen-Hui
Jiang, Ben-Chun
Chen, Xin-Ying
Dai, Jing-Wei
Tong, Yu-Xin
Yang, Ren
Sun, Wei
Wang, Qiang
author_facet Ji, Ce
Zhao, Ying
Kou, You-Wei
Shao, Hua
Guo, Lin
Bao, Chen-Hui
Jiang, Ben-Chun
Chen, Xin-Ying
Dai, Jing-Wei
Tong, Yu-Xin
Yang, Ren
Sun, Wei
Wang, Qiang
author_sort Ji, Ce
collection PubMed
description Gastric cancer (GC) is one of the most common cancers in the world. The cathepsin F (CTSF) gene has recently been found to participate in the progression of several types of cancer. However, the clinical characteristics and function of CTSF in GC as well as its molecular mechanisms are not clear. Six GC cell lines and 44 paired adjacent noncancerous and GC tissue samples were used to assess CTSF expression by quantitative polymerase chain reaction (qPCR). We used lentivirus-mediated small hairpin RNA (Lenti-shRNA) against CTSF to knock down the expression of CTSF in GC cells. Western blot and qPCR were used to analyze the mRNA and related protein expression. The biological phenotypes of gastric cells were examined by cell proliferation and apoptosis assays. Microarray-based mRNA expression profile screening was also performed to evaluate the potential molecular pathways in which CTSF may be involved. The CTSF mRNA level was associated with tumor differentiation, depth of tumor invasion, and lymph node metastasis. Downregulation of CTSF expression efficiently inhibited apoptosis and promoted the proliferation of GC cells. Moreover, a total of 1,117 upregulated mRNAs and 1,143 downregulated mRNAs were identified as differentially expressed genes (DEGs). Further analysis identified the involvement of these mRNAs in cancer-related pathways and various other biological processes. Nine DEGs in cancer-related pathways and three downstream genes in the apoptosis pathway were validated by Western blot, which was mainly in agreement with the microarray data. To our knowledge, this is the first report investigating the effect of CTSF on the growth and apoptosis in GC cells and its clinical significance. The CTSF gene may function as a tumor suppressor in GC and may be a potential therapeutic target in the treatment of GC.
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spelling pubmed-78445612021-02-16 Cathepsin F Knockdown Induces Proliferation and Inhibits Apoptosis in Gastric Cancer Cells Ji, Ce Zhao, Ying Kou, You-Wei Shao, Hua Guo, Lin Bao, Chen-Hui Jiang, Ben-Chun Chen, Xin-Ying Dai, Jing-Wei Tong, Yu-Xin Yang, Ren Sun, Wei Wang, Qiang Oncol Res Article Gastric cancer (GC) is one of the most common cancers in the world. The cathepsin F (CTSF) gene has recently been found to participate in the progression of several types of cancer. However, the clinical characteristics and function of CTSF in GC as well as its molecular mechanisms are not clear. Six GC cell lines and 44 paired adjacent noncancerous and GC tissue samples were used to assess CTSF expression by quantitative polymerase chain reaction (qPCR). We used lentivirus-mediated small hairpin RNA (Lenti-shRNA) against CTSF to knock down the expression of CTSF in GC cells. Western blot and qPCR were used to analyze the mRNA and related protein expression. The biological phenotypes of gastric cells were examined by cell proliferation and apoptosis assays. Microarray-based mRNA expression profile screening was also performed to evaluate the potential molecular pathways in which CTSF may be involved. The CTSF mRNA level was associated with tumor differentiation, depth of tumor invasion, and lymph node metastasis. Downregulation of CTSF expression efficiently inhibited apoptosis and promoted the proliferation of GC cells. Moreover, a total of 1,117 upregulated mRNAs and 1,143 downregulated mRNAs were identified as differentially expressed genes (DEGs). Further analysis identified the involvement of these mRNAs in cancer-related pathways and various other biological processes. Nine DEGs in cancer-related pathways and three downstream genes in the apoptosis pathway were validated by Western blot, which was mainly in agreement with the microarray data. To our knowledge, this is the first report investigating the effect of CTSF on the growth and apoptosis in GC cells and its clinical significance. The CTSF gene may function as a tumor suppressor in GC and may be a potential therapeutic target in the treatment of GC. Cognizant Communication Corporation 2018-01-19 /pmc/articles/PMC7844561/ /pubmed/28474574 http://dx.doi.org/10.3727/096504017X14928634401204 Text en Copyright © 2018 Cognizant, LLC. http://creativecommons.org/licenses/by-nc-nd/4.0/ This article is licensed under a Creative Commons Attribution-NonCommercial NoDerivatives 4.0 International License.
spellingShingle Article
Ji, Ce
Zhao, Ying
Kou, You-Wei
Shao, Hua
Guo, Lin
Bao, Chen-Hui
Jiang, Ben-Chun
Chen, Xin-Ying
Dai, Jing-Wei
Tong, Yu-Xin
Yang, Ren
Sun, Wei
Wang, Qiang
Cathepsin F Knockdown Induces Proliferation and Inhibits Apoptosis in Gastric Cancer Cells
title Cathepsin F Knockdown Induces Proliferation and Inhibits Apoptosis in Gastric Cancer Cells
title_full Cathepsin F Knockdown Induces Proliferation and Inhibits Apoptosis in Gastric Cancer Cells
title_fullStr Cathepsin F Knockdown Induces Proliferation and Inhibits Apoptosis in Gastric Cancer Cells
title_full_unstemmed Cathepsin F Knockdown Induces Proliferation and Inhibits Apoptosis in Gastric Cancer Cells
title_short Cathepsin F Knockdown Induces Proliferation and Inhibits Apoptosis in Gastric Cancer Cells
title_sort cathepsin f knockdown induces proliferation and inhibits apoptosis in gastric cancer cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7844561/
https://www.ncbi.nlm.nih.gov/pubmed/28474574
http://dx.doi.org/10.3727/096504017X14928634401204
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