Optimized protocol for gene editing in adipocytes using CRISPR-Cas9 technology

We present a detailed protocol for gene editing in adipocytes using the CRISPR-Cas technology. This protocol describes sgRNA design, preparation of lentiCRISPR-sgRNA vectors, functional validation of sgRNAs, preparation of lentiviruses, and lentiviruses transduction in adipocytes. Moreover, an optim...

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Detalles Bibliográficos
Autores principales: Qiu, Yan, Ding, Qiurong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7844572/
https://www.ncbi.nlm.nih.gov/pubmed/33554142
http://dx.doi.org/10.1016/j.xpro.2021.100307

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