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Let-7c Inhibits the Proliferation, Invasion, and Migration of Glioma Cells via Targeting E2F5

As a member of the miRNA family, let-7c has been identified as a tumor suppressor in many cancers. However, the molecular biological function of let-7c in glioma has not been elucidated. The aim of this study was to explore let-7c expression levels and evaluate its function in glioma cells. We first...

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Autores principales: Huang, Mengyi, Gong, Xin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cognizant Communication Corporation 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7844676/
https://www.ncbi.nlm.nih.gov/pubmed/29362021
http://dx.doi.org/10.3727/096504018X15164123839400
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author Huang, Mengyi
Gong, Xin
author_facet Huang, Mengyi
Gong, Xin
author_sort Huang, Mengyi
collection PubMed
description As a member of the miRNA family, let-7c has been identified as a tumor suppressor in many cancers. However, the molecular biological function of let-7c in glioma has not been elucidated. The aim of this study was to explore let-7c expression levels and evaluate its function in glioma cells. We first measured the expression of let-7c in four glioma cell lines and a normal cell line by quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR), and the results showed that let-7c was downregulated in glioma cells. By applying gain-of-function and loss-of-function assays, the experiments suggested that dysregulation of let-7c could obviously affect cell proliferation, metastasis, and invasion. Based on online bioinformatics analysis and Dual-Luciferase Reporter assays, we found that E2F5 was a target gene of let-7c and contributed to the function of let-7c in glioma cells. Our investigations indicated that loss of let-7c contributed to the progression of glioma cells.
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spelling pubmed-78446762021-02-16 Let-7c Inhibits the Proliferation, Invasion, and Migration of Glioma Cells via Targeting E2F5 Huang, Mengyi Gong, Xin Oncol Res Article As a member of the miRNA family, let-7c has been identified as a tumor suppressor in many cancers. However, the molecular biological function of let-7c in glioma has not been elucidated. The aim of this study was to explore let-7c expression levels and evaluate its function in glioma cells. We first measured the expression of let-7c in four glioma cell lines and a normal cell line by quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR), and the results showed that let-7c was downregulated in glioma cells. By applying gain-of-function and loss-of-function assays, the experiments suggested that dysregulation of let-7c could obviously affect cell proliferation, metastasis, and invasion. Based on online bioinformatics analysis and Dual-Luciferase Reporter assays, we found that E2F5 was a target gene of let-7c and contributed to the function of let-7c in glioma cells. Our investigations indicated that loss of let-7c contributed to the progression of glioma cells. Cognizant Communication Corporation 2018-08-23 /pmc/articles/PMC7844676/ /pubmed/29362021 http://dx.doi.org/10.3727/096504018X15164123839400 Text en Copyright © 2018 Cognizant, LLC. http://creativecommons.org/licenses/by-nc-nd/4.0/ This article is licensed under a Creative Commons Attribution-NonCommercial NoDerivatives 4.0 International License.
spellingShingle Article
Huang, Mengyi
Gong, Xin
Let-7c Inhibits the Proliferation, Invasion, and Migration of Glioma Cells via Targeting E2F5
title Let-7c Inhibits the Proliferation, Invasion, and Migration of Glioma Cells via Targeting E2F5
title_full Let-7c Inhibits the Proliferation, Invasion, and Migration of Glioma Cells via Targeting E2F5
title_fullStr Let-7c Inhibits the Proliferation, Invasion, and Migration of Glioma Cells via Targeting E2F5
title_full_unstemmed Let-7c Inhibits the Proliferation, Invasion, and Migration of Glioma Cells via Targeting E2F5
title_short Let-7c Inhibits the Proliferation, Invasion, and Migration of Glioma Cells via Targeting E2F5
title_sort let-7c inhibits the proliferation, invasion, and migration of glioma cells via targeting e2f5
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7844676/
https://www.ncbi.nlm.nih.gov/pubmed/29362021
http://dx.doi.org/10.3727/096504018X15164123839400
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