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Let-7c Inhibits the Proliferation, Invasion, and Migration of Glioma Cells via Targeting E2F5
As a member of the miRNA family, let-7c has been identified as a tumor suppressor in many cancers. However, the molecular biological function of let-7c in glioma has not been elucidated. The aim of this study was to explore let-7c expression levels and evaluate its function in glioma cells. We first...
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Cognizant Communication Corporation
2018
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7844676/ https://www.ncbi.nlm.nih.gov/pubmed/29362021 http://dx.doi.org/10.3727/096504018X15164123839400 |
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author | Huang, Mengyi Gong, Xin |
author_facet | Huang, Mengyi Gong, Xin |
author_sort | Huang, Mengyi |
collection | PubMed |
description | As a member of the miRNA family, let-7c has been identified as a tumor suppressor in many cancers. However, the molecular biological function of let-7c in glioma has not been elucidated. The aim of this study was to explore let-7c expression levels and evaluate its function in glioma cells. We first measured the expression of let-7c in four glioma cell lines and a normal cell line by quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR), and the results showed that let-7c was downregulated in glioma cells. By applying gain-of-function and loss-of-function assays, the experiments suggested that dysregulation of let-7c could obviously affect cell proliferation, metastasis, and invasion. Based on online bioinformatics analysis and Dual-Luciferase Reporter assays, we found that E2F5 was a target gene of let-7c and contributed to the function of let-7c in glioma cells. Our investigations indicated that loss of let-7c contributed to the progression of glioma cells. |
format | Online Article Text |
id | pubmed-7844676 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Cognizant Communication Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-78446762021-02-16 Let-7c Inhibits the Proliferation, Invasion, and Migration of Glioma Cells via Targeting E2F5 Huang, Mengyi Gong, Xin Oncol Res Article As a member of the miRNA family, let-7c has been identified as a tumor suppressor in many cancers. However, the molecular biological function of let-7c in glioma has not been elucidated. The aim of this study was to explore let-7c expression levels and evaluate its function in glioma cells. We first measured the expression of let-7c in four glioma cell lines and a normal cell line by quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR), and the results showed that let-7c was downregulated in glioma cells. By applying gain-of-function and loss-of-function assays, the experiments suggested that dysregulation of let-7c could obviously affect cell proliferation, metastasis, and invasion. Based on online bioinformatics analysis and Dual-Luciferase Reporter assays, we found that E2F5 was a target gene of let-7c and contributed to the function of let-7c in glioma cells. Our investigations indicated that loss of let-7c contributed to the progression of glioma cells. Cognizant Communication Corporation 2018-08-23 /pmc/articles/PMC7844676/ /pubmed/29362021 http://dx.doi.org/10.3727/096504018X15164123839400 Text en Copyright © 2018 Cognizant, LLC. http://creativecommons.org/licenses/by-nc-nd/4.0/ This article is licensed under a Creative Commons Attribution-NonCommercial NoDerivatives 4.0 International License. |
spellingShingle | Article Huang, Mengyi Gong, Xin Let-7c Inhibits the Proliferation, Invasion, and Migration of Glioma Cells via Targeting E2F5 |
title | Let-7c Inhibits the Proliferation, Invasion, and Migration of Glioma Cells via Targeting E2F5 |
title_full | Let-7c Inhibits the Proliferation, Invasion, and Migration of Glioma Cells via Targeting E2F5 |
title_fullStr | Let-7c Inhibits the Proliferation, Invasion, and Migration of Glioma Cells via Targeting E2F5 |
title_full_unstemmed | Let-7c Inhibits the Proliferation, Invasion, and Migration of Glioma Cells via Targeting E2F5 |
title_short | Let-7c Inhibits the Proliferation, Invasion, and Migration of Glioma Cells via Targeting E2F5 |
title_sort | let-7c inhibits the proliferation, invasion, and migration of glioma cells via targeting e2f5 |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7844676/ https://www.ncbi.nlm.nih.gov/pubmed/29362021 http://dx.doi.org/10.3727/096504018X15164123839400 |
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