Cargando…

The Downregulation of miR-200c Promotes Lactate Dehydrogenase A Expression and Non-Small Cell Lung Cancer Progression

This study was aimed to investigate the function and mechanism of microRNA-200c (miR-200c) in the progression of non-small cell lung cancer (NSCLC). A total of 76 patients diagnosed as having NSCLC were enrolled in this study. The expression level of miR-200c in NSCLC tissues and cell lines was inve...

Descripción completa

Detalles Bibliográficos
Autores principales: Lei, Wei, Kang, Wang, Nan, Yang, Lei, Zhang, Zhongdong, Li, Demin, Li, Lei, Sun, Hairong, Huang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cognizant Communication Corporation 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7844790/
https://www.ncbi.nlm.nih.gov/pubmed/29321091
http://dx.doi.org/10.3727/096504018X15151486241153
_version_ 1783644423529168896
author Lei, Wei
Kang, Wang
Nan, Yang
Lei, Zhang
Zhongdong, Li
Demin, Li
Lei, Sun
Hairong, Huang
author_facet Lei, Wei
Kang, Wang
Nan, Yang
Lei, Zhang
Zhongdong, Li
Demin, Li
Lei, Sun
Hairong, Huang
author_sort Lei, Wei
collection PubMed
description This study was aimed to investigate the function and mechanism of microRNA-200c (miR-200c) in the progression of non-small cell lung cancer (NSCLC). A total of 76 patients diagnosed as having NSCLC were enrolled in this study. The expression level of miR-200c in NSCLC tissues and cell lines was investigated using the quantitative real-time polymerase chain reaction (RT-qPCR) method. We found that the expression of miR-200c was significantly reduced in NSCLC tissues and cell lines compared with normal lung tissues and the human bronchial epithelial cell line. Overexpression of miR-200c using the miR-200c mimic significantly suppressed cell proliferation and migration of NSCLC cell lines. The results of the luciferase reporter assay identified lactate dehydrogenase A (LDHA) as a direct target of miR-200c. The expression of LDHA was shown to be suppressed in NSCLC cell lines with miR-200c mimic transfection. Furthermore, the transfection of small interfering RNA (siRNA) targeting LDHA suppressed the proliferation and migration of NSCLC cell lines. In summary, our results presented in this study suggested that miR-200c was able to inhibit the proliferation and migration of NSCLC cells by downregulating LDHA. Therefore, miR-200c may be considered as a potential candidate for the treatment of NSCLC.
format Online
Article
Text
id pubmed-7844790
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher Cognizant Communication Corporation
record_format MEDLINE/PubMed
spelling pubmed-78447902021-02-16 The Downregulation of miR-200c Promotes Lactate Dehydrogenase A Expression and Non-Small Cell Lung Cancer Progression Lei, Wei Kang, Wang Nan, Yang Lei, Zhang Zhongdong, Li Demin, Li Lei, Sun Hairong, Huang Oncol Res Article This study was aimed to investigate the function and mechanism of microRNA-200c (miR-200c) in the progression of non-small cell lung cancer (NSCLC). A total of 76 patients diagnosed as having NSCLC were enrolled in this study. The expression level of miR-200c in NSCLC tissues and cell lines was investigated using the quantitative real-time polymerase chain reaction (RT-qPCR) method. We found that the expression of miR-200c was significantly reduced in NSCLC tissues and cell lines compared with normal lung tissues and the human bronchial epithelial cell line. Overexpression of miR-200c using the miR-200c mimic significantly suppressed cell proliferation and migration of NSCLC cell lines. The results of the luciferase reporter assay identified lactate dehydrogenase A (LDHA) as a direct target of miR-200c. The expression of LDHA was shown to be suppressed in NSCLC cell lines with miR-200c mimic transfection. Furthermore, the transfection of small interfering RNA (siRNA) targeting LDHA suppressed the proliferation and migration of NSCLC cell lines. In summary, our results presented in this study suggested that miR-200c was able to inhibit the proliferation and migration of NSCLC cells by downregulating LDHA. Therefore, miR-200c may be considered as a potential candidate for the treatment of NSCLC. Cognizant Communication Corporation 2018-08-23 /pmc/articles/PMC7844790/ /pubmed/29321091 http://dx.doi.org/10.3727/096504018X15151486241153 Text en Copyright © 2018 Cognizant, LLC. http://creativecommons.org/licenses/by-nc-nd/4.0/ This article is licensed under a Creative Commons Attribution-NonCommercial NoDerivatives 4.0 International License.
spellingShingle Article
Lei, Wei
Kang, Wang
Nan, Yang
Lei, Zhang
Zhongdong, Li
Demin, Li
Lei, Sun
Hairong, Huang
The Downregulation of miR-200c Promotes Lactate Dehydrogenase A Expression and Non-Small Cell Lung Cancer Progression
title The Downregulation of miR-200c Promotes Lactate Dehydrogenase A Expression and Non-Small Cell Lung Cancer Progression
title_full The Downregulation of miR-200c Promotes Lactate Dehydrogenase A Expression and Non-Small Cell Lung Cancer Progression
title_fullStr The Downregulation of miR-200c Promotes Lactate Dehydrogenase A Expression and Non-Small Cell Lung Cancer Progression
title_full_unstemmed The Downregulation of miR-200c Promotes Lactate Dehydrogenase A Expression and Non-Small Cell Lung Cancer Progression
title_short The Downregulation of miR-200c Promotes Lactate Dehydrogenase A Expression and Non-Small Cell Lung Cancer Progression
title_sort downregulation of mir-200c promotes lactate dehydrogenase a expression and non-small cell lung cancer progression
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7844790/
https://www.ncbi.nlm.nih.gov/pubmed/29321091
http://dx.doi.org/10.3727/096504018X15151486241153
work_keys_str_mv AT leiwei thedownregulationofmir200cpromoteslactatedehydrogenaseaexpressionandnonsmallcelllungcancerprogression
AT kangwang thedownregulationofmir200cpromoteslactatedehydrogenaseaexpressionandnonsmallcelllungcancerprogression
AT nanyang thedownregulationofmir200cpromoteslactatedehydrogenaseaexpressionandnonsmallcelllungcancerprogression
AT leizhang thedownregulationofmir200cpromoteslactatedehydrogenaseaexpressionandnonsmallcelllungcancerprogression
AT zhongdongli thedownregulationofmir200cpromoteslactatedehydrogenaseaexpressionandnonsmallcelllungcancerprogression
AT deminli thedownregulationofmir200cpromoteslactatedehydrogenaseaexpressionandnonsmallcelllungcancerprogression
AT leisun thedownregulationofmir200cpromoteslactatedehydrogenaseaexpressionandnonsmallcelllungcancerprogression
AT haironghuang thedownregulationofmir200cpromoteslactatedehydrogenaseaexpressionandnonsmallcelllungcancerprogression
AT leiwei downregulationofmir200cpromoteslactatedehydrogenaseaexpressionandnonsmallcelllungcancerprogression
AT kangwang downregulationofmir200cpromoteslactatedehydrogenaseaexpressionandnonsmallcelllungcancerprogression
AT nanyang downregulationofmir200cpromoteslactatedehydrogenaseaexpressionandnonsmallcelllungcancerprogression
AT leizhang downregulationofmir200cpromoteslactatedehydrogenaseaexpressionandnonsmallcelllungcancerprogression
AT zhongdongli downregulationofmir200cpromoteslactatedehydrogenaseaexpressionandnonsmallcelllungcancerprogression
AT deminli downregulationofmir200cpromoteslactatedehydrogenaseaexpressionandnonsmallcelllungcancerprogression
AT leisun downregulationofmir200cpromoteslactatedehydrogenaseaexpressionandnonsmallcelllungcancerprogression
AT haironghuang downregulationofmir200cpromoteslactatedehydrogenaseaexpressionandnonsmallcelllungcancerprogression