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Human Dental Pulp-Derived Mesenchymal Stem Cell Potential to Differentiate into Smooth Muscle-Like Cells In Vitro
Previous studies have shown that mesenchymal stem cells (MSCs) derived from various tissue sources can be differentiated into smooth muscle-like cells (SMLCs) in vitro. In this paper, dental pulp-derived mesenchymal stem cells (DPSCs) were evaluated for their differentiation ability towards smooth m...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7846403/ https://www.ncbi.nlm.nih.gov/pubmed/33553433 http://dx.doi.org/10.1155/2021/8858412 |
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author | Ha, Jinhee Bharti, Dinesh Kang, Young-Hoon Lee, Sang-Yeob Oh, Seong-Ju Kim, Saet-Byul Jo, Chan-Hee Son, Jang-Ho Sung, Iel-Yong Cho, Yeong-Cheol Rho, Gyu-Jin Park, Jeong-Kil |
author_facet | Ha, Jinhee Bharti, Dinesh Kang, Young-Hoon Lee, Sang-Yeob Oh, Seong-Ju Kim, Saet-Byul Jo, Chan-Hee Son, Jang-Ho Sung, Iel-Yong Cho, Yeong-Cheol Rho, Gyu-Jin Park, Jeong-Kil |
author_sort | Ha, Jinhee |
collection | PubMed |
description | Previous studies have shown that mesenchymal stem cells (MSCs) derived from various tissue sources can be differentiated into smooth muscle-like cells (SMLCs) in vitro. In this paper, dental pulp-derived mesenchymal stem cells (DPSCs) were evaluated for their differentiation ability towards smooth muscle-like cells (SMLCs) under the effect of widely used cytokines (TGF-β1 and PDGF-BB) with special focus on different culturing environments. For this purpose, both the commercially used culturing plates (Norm-c) and 0.1% gelatin-precoated (Gel-c) plates were used. Isolated cells displayed plastic adherence, pluripotency and cell surface marker profiling, and adipogenic and osteogenic differentiation potential with lineage specific marker expression. Differentiated cells induced under different culturing plates showed successful differentiation into SMLCs by positively expressing smooth muscle cell (SMC) specific markers both at the mRNA and protein levels. Gelatin coating could substantially enhance DPSC differentiation potential than Norm-c-induced cells. However, the absence of mature marker MHY-11 by immunostaining results from all treatment groups further indicated the development of immature and synthetic SMLCs. Finally, it was concluded that DPSC differentiation ability into SMLCs can be enhanced under cytokine treatment as well as by altering the cellular niche by precoating the culturing plates with suitable substrates. However, to get fully functional, contractile, and mature SMLCs, still many different cytokine cocktail combinations and more suitable coating substrates will be needed. |
format | Online Article Text |
id | pubmed-7846403 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Hindawi |
record_format | MEDLINE/PubMed |
spelling | pubmed-78464032021-02-04 Human Dental Pulp-Derived Mesenchymal Stem Cell Potential to Differentiate into Smooth Muscle-Like Cells In Vitro Ha, Jinhee Bharti, Dinesh Kang, Young-Hoon Lee, Sang-Yeob Oh, Seong-Ju Kim, Saet-Byul Jo, Chan-Hee Son, Jang-Ho Sung, Iel-Yong Cho, Yeong-Cheol Rho, Gyu-Jin Park, Jeong-Kil Biomed Res Int Research Article Previous studies have shown that mesenchymal stem cells (MSCs) derived from various tissue sources can be differentiated into smooth muscle-like cells (SMLCs) in vitro. In this paper, dental pulp-derived mesenchymal stem cells (DPSCs) were evaluated for their differentiation ability towards smooth muscle-like cells (SMLCs) under the effect of widely used cytokines (TGF-β1 and PDGF-BB) with special focus on different culturing environments. For this purpose, both the commercially used culturing plates (Norm-c) and 0.1% gelatin-precoated (Gel-c) plates were used. Isolated cells displayed plastic adherence, pluripotency and cell surface marker profiling, and adipogenic and osteogenic differentiation potential with lineage specific marker expression. Differentiated cells induced under different culturing plates showed successful differentiation into SMLCs by positively expressing smooth muscle cell (SMC) specific markers both at the mRNA and protein levels. Gelatin coating could substantially enhance DPSC differentiation potential than Norm-c-induced cells. However, the absence of mature marker MHY-11 by immunostaining results from all treatment groups further indicated the development of immature and synthetic SMLCs. Finally, it was concluded that DPSC differentiation ability into SMLCs can be enhanced under cytokine treatment as well as by altering the cellular niche by precoating the culturing plates with suitable substrates. However, to get fully functional, contractile, and mature SMLCs, still many different cytokine cocktail combinations and more suitable coating substrates will be needed. Hindawi 2021-01-22 /pmc/articles/PMC7846403/ /pubmed/33553433 http://dx.doi.org/10.1155/2021/8858412 Text en Copyright © 2021 Jinhee Ha et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Ha, Jinhee Bharti, Dinesh Kang, Young-Hoon Lee, Sang-Yeob Oh, Seong-Ju Kim, Saet-Byul Jo, Chan-Hee Son, Jang-Ho Sung, Iel-Yong Cho, Yeong-Cheol Rho, Gyu-Jin Park, Jeong-Kil Human Dental Pulp-Derived Mesenchymal Stem Cell Potential to Differentiate into Smooth Muscle-Like Cells In Vitro |
title | Human Dental Pulp-Derived Mesenchymal Stem Cell Potential to Differentiate into Smooth Muscle-Like Cells In Vitro |
title_full | Human Dental Pulp-Derived Mesenchymal Stem Cell Potential to Differentiate into Smooth Muscle-Like Cells In Vitro |
title_fullStr | Human Dental Pulp-Derived Mesenchymal Stem Cell Potential to Differentiate into Smooth Muscle-Like Cells In Vitro |
title_full_unstemmed | Human Dental Pulp-Derived Mesenchymal Stem Cell Potential to Differentiate into Smooth Muscle-Like Cells In Vitro |
title_short | Human Dental Pulp-Derived Mesenchymal Stem Cell Potential to Differentiate into Smooth Muscle-Like Cells In Vitro |
title_sort | human dental pulp-derived mesenchymal stem cell potential to differentiate into smooth muscle-like cells in vitro |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7846403/ https://www.ncbi.nlm.nih.gov/pubmed/33553433 http://dx.doi.org/10.1155/2021/8858412 |
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