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Genome-Wide Investigation and Expression Profiling Under Abiotic Stresses of a Soybean Unknown Function (DUF21) and Cystathionine-β-Synthase (CBS) Domain-Containing Protein Family

Cystathionine-β-synthase (CBS) domain-containing proteins (CDCPs) constitute a large family in plants, and members of this family have been implicated in a variety of biological processes. However, the precise functions and the underlying mechanisms of most members of this family in plants remain to...

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Autores principales: Hao, Qingnan, Yang, Yanyan, Shan, Zhihui, Chen, Haifeng, Zhang, Chanjuan, Chen, Limiao, Yuan, Songli, Zhang, Xiaojuan, Chen, Shuilian, Yang, Zhonglu, Qiu, Dezhen, Zhou, Xinan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer US 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7846513/
https://www.ncbi.nlm.nih.gov/pubmed/32778975
http://dx.doi.org/10.1007/s10528-020-09991-w
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author Hao, Qingnan
Yang, Yanyan
Shan, Zhihui
Chen, Haifeng
Zhang, Chanjuan
Chen, Limiao
Yuan, Songli
Zhang, Xiaojuan
Chen, Shuilian
Yang, Zhonglu
Qiu, Dezhen
Zhou, Xinan
author_facet Hao, Qingnan
Yang, Yanyan
Shan, Zhihui
Chen, Haifeng
Zhang, Chanjuan
Chen, Limiao
Yuan, Songli
Zhang, Xiaojuan
Chen, Shuilian
Yang, Zhonglu
Qiu, Dezhen
Zhou, Xinan
author_sort Hao, Qingnan
collection PubMed
description Cystathionine-β-synthase (CBS) domain-containing proteins (CDCPs) constitute a large family in plants, and members of this family have been implicated in a variety of biological processes. However, the precise functions and the underlying mechanisms of most members of this family in plants remain to be elucidated. CBSDUF proteins belong to the CDCP superfamily, which contains one domain of unknown function (DUF21) and an N terminus that is adjacent to two intracellular CBS domains. In this study, a comprehensive genome database analysis of soybean was performed to investigate the role(s) of these CBSDUFs and to explore their nomenclature, classification, chromosomal distribution, exon–intron organization, protein structure, and phylogenetic relationships; the analysis identified a total of 18 putative CBSDUF genes. Using specific protein domains and phylogenetic analysis, the CBSDUF gene family was subdivided into eight groups. The soybean CBSDUF genes showed an uneven distribution on 12 chromosomes of Glycine max. RNA-seq transcriptome data from different tissues in public databases revealed tissue-specific and differential expression profiles of the GmCBSDUFs, and qPCR analysis revealed that certain groups of soybean CBSDUFs are likely involved in specific stress responses. In addition, GmCBSDUF3 transgenic Arabidopsis was subjected to phenotypic analysis under NaCl, PEG, and ABA stress treatments. The overexpression of GmCBSDUF3 could enhance tolerance to drought and salt stress in Arabidopsis. This study presents a first comprehensive look at soybean CBSDUF proteins and provides valuable resources for functionally elucidating this protein subgroup within the CBS domain-containing protein family. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s10528-020-09991-w) contains supplementary material, which is available to authorized users.
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spelling pubmed-78465132021-02-11 Genome-Wide Investigation and Expression Profiling Under Abiotic Stresses of a Soybean Unknown Function (DUF21) and Cystathionine-β-Synthase (CBS) Domain-Containing Protein Family Hao, Qingnan Yang, Yanyan Shan, Zhihui Chen, Haifeng Zhang, Chanjuan Chen, Limiao Yuan, Songli Zhang, Xiaojuan Chen, Shuilian Yang, Zhonglu Qiu, Dezhen Zhou, Xinan Biochem Genet Original Article Cystathionine-β-synthase (CBS) domain-containing proteins (CDCPs) constitute a large family in plants, and members of this family have been implicated in a variety of biological processes. However, the precise functions and the underlying mechanisms of most members of this family in plants remain to be elucidated. CBSDUF proteins belong to the CDCP superfamily, which contains one domain of unknown function (DUF21) and an N terminus that is adjacent to two intracellular CBS domains. In this study, a comprehensive genome database analysis of soybean was performed to investigate the role(s) of these CBSDUFs and to explore their nomenclature, classification, chromosomal distribution, exon–intron organization, protein structure, and phylogenetic relationships; the analysis identified a total of 18 putative CBSDUF genes. Using specific protein domains and phylogenetic analysis, the CBSDUF gene family was subdivided into eight groups. The soybean CBSDUF genes showed an uneven distribution on 12 chromosomes of Glycine max. RNA-seq transcriptome data from different tissues in public databases revealed tissue-specific and differential expression profiles of the GmCBSDUFs, and qPCR analysis revealed that certain groups of soybean CBSDUFs are likely involved in specific stress responses. In addition, GmCBSDUF3 transgenic Arabidopsis was subjected to phenotypic analysis under NaCl, PEG, and ABA stress treatments. The overexpression of GmCBSDUF3 could enhance tolerance to drought and salt stress in Arabidopsis. This study presents a first comprehensive look at soybean CBSDUF proteins and provides valuable resources for functionally elucidating this protein subgroup within the CBS domain-containing protein family. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s10528-020-09991-w) contains supplementary material, which is available to authorized users. Springer US 2020-08-10 2021 /pmc/articles/PMC7846513/ /pubmed/32778975 http://dx.doi.org/10.1007/s10528-020-09991-w Text en © The Author(s) 2020 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Original Article
Hao, Qingnan
Yang, Yanyan
Shan, Zhihui
Chen, Haifeng
Zhang, Chanjuan
Chen, Limiao
Yuan, Songli
Zhang, Xiaojuan
Chen, Shuilian
Yang, Zhonglu
Qiu, Dezhen
Zhou, Xinan
Genome-Wide Investigation and Expression Profiling Under Abiotic Stresses of a Soybean Unknown Function (DUF21) and Cystathionine-β-Synthase (CBS) Domain-Containing Protein Family
title Genome-Wide Investigation and Expression Profiling Under Abiotic Stresses of a Soybean Unknown Function (DUF21) and Cystathionine-β-Synthase (CBS) Domain-Containing Protein Family
title_full Genome-Wide Investigation and Expression Profiling Under Abiotic Stresses of a Soybean Unknown Function (DUF21) and Cystathionine-β-Synthase (CBS) Domain-Containing Protein Family
title_fullStr Genome-Wide Investigation and Expression Profiling Under Abiotic Stresses of a Soybean Unknown Function (DUF21) and Cystathionine-β-Synthase (CBS) Domain-Containing Protein Family
title_full_unstemmed Genome-Wide Investigation and Expression Profiling Under Abiotic Stresses of a Soybean Unknown Function (DUF21) and Cystathionine-β-Synthase (CBS) Domain-Containing Protein Family
title_short Genome-Wide Investigation and Expression Profiling Under Abiotic Stresses of a Soybean Unknown Function (DUF21) and Cystathionine-β-Synthase (CBS) Domain-Containing Protein Family
title_sort genome-wide investigation and expression profiling under abiotic stresses of a soybean unknown function (duf21) and cystathionine-β-synthase (cbs) domain-containing protein family
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7846513/
https://www.ncbi.nlm.nih.gov/pubmed/32778975
http://dx.doi.org/10.1007/s10528-020-09991-w
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