A single-dose live attenuated chimeric vaccine candidate against Zika virus

The mosquito-borne Zika virus is an emerging pathogen from the Flavivirus genus for which there are no approved antivirals or vaccines. Using the clinically validated PDK-53 dengue virus vaccine strain as a backbone, we created a chimeric dengue/Zika virus, VacDZ, as a live attenuated vaccine candid...

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Autores principales: Chin, Wei-Xin, Lee, Regina Ching Hua, Kaur, Parveen, Lew, Tian Sheng, Yogarajah, Thinesshwary, Kong, Hao Yuin, Teo, Zi-Yun, Salim, Cyrill Kafi, Zhang, Rong-Rong, Li, Xiao-Feng, Alonso, Sylvie, Qin, Cheng-Feng, Chu, Justin Jang Hann
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7846741/
https://www.ncbi.nlm.nih.gov/pubmed/33514743
http://dx.doi.org/10.1038/s41541-021-00282-y
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author Chin, Wei-Xin
Lee, Regina Ching Hua
Kaur, Parveen
Lew, Tian Sheng
Yogarajah, Thinesshwary
Kong, Hao Yuin
Teo, Zi-Yun
Salim, Cyrill Kafi
Zhang, Rong-Rong
Li, Xiao-Feng
Alonso, Sylvie
Qin, Cheng-Feng
Chu, Justin Jang Hann
author_facet Chin, Wei-Xin
Lee, Regina Ching Hua
Kaur, Parveen
Lew, Tian Sheng
Yogarajah, Thinesshwary
Kong, Hao Yuin
Teo, Zi-Yun
Salim, Cyrill Kafi
Zhang, Rong-Rong
Li, Xiao-Feng
Alonso, Sylvie
Qin, Cheng-Feng
Chu, Justin Jang Hann
author_sort Chin, Wei-Xin
collection PubMed
description The mosquito-borne Zika virus is an emerging pathogen from the Flavivirus genus for which there are no approved antivirals or vaccines. Using the clinically validated PDK-53 dengue virus vaccine strain as a backbone, we created a chimeric dengue/Zika virus, VacDZ, as a live attenuated vaccine candidate against Zika virus. VacDZ demonstrates key markers of attenuation: small plaque phenotype, temperature sensitivity, attenuation of neurovirulence in suckling mice, and attenuation of pathogenicity in interferon deficient adult AG129 mice. VacDZ may be administered as a traditional live virus vaccine, or as a DNA-launched vaccine that produces live VacDZ in vivo after delivery. Both vaccine formulations induce a protective immune response against Zika virus in AG129 mice, which includes neutralising antibodies and a strong Th1 response. This study demonstrates that VacDZ is a safe and effective vaccine candidate against Zika virus.
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spelling pubmed-78467412021-02-08 A single-dose live attenuated chimeric vaccine candidate against Zika virus Chin, Wei-Xin Lee, Regina Ching Hua Kaur, Parveen Lew, Tian Sheng Yogarajah, Thinesshwary Kong, Hao Yuin Teo, Zi-Yun Salim, Cyrill Kafi Zhang, Rong-Rong Li, Xiao-Feng Alonso, Sylvie Qin, Cheng-Feng Chu, Justin Jang Hann NPJ Vaccines Article The mosquito-borne Zika virus is an emerging pathogen from the Flavivirus genus for which there are no approved antivirals or vaccines. Using the clinically validated PDK-53 dengue virus vaccine strain as a backbone, we created a chimeric dengue/Zika virus, VacDZ, as a live attenuated vaccine candidate against Zika virus. VacDZ demonstrates key markers of attenuation: small plaque phenotype, temperature sensitivity, attenuation of neurovirulence in suckling mice, and attenuation of pathogenicity in interferon deficient adult AG129 mice. VacDZ may be administered as a traditional live virus vaccine, or as a DNA-launched vaccine that produces live VacDZ in vivo after delivery. Both vaccine formulations induce a protective immune response against Zika virus in AG129 mice, which includes neutralising antibodies and a strong Th1 response. This study demonstrates that VacDZ is a safe and effective vaccine candidate against Zika virus. Nature Publishing Group UK 2021-01-29 /pmc/articles/PMC7846741/ /pubmed/33514743 http://dx.doi.org/10.1038/s41541-021-00282-y Text en © The Author(s) 2021 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Chin, Wei-Xin
Lee, Regina Ching Hua
Kaur, Parveen
Lew, Tian Sheng
Yogarajah, Thinesshwary
Kong, Hao Yuin
Teo, Zi-Yun
Salim, Cyrill Kafi
Zhang, Rong-Rong
Li, Xiao-Feng
Alonso, Sylvie
Qin, Cheng-Feng
Chu, Justin Jang Hann
A single-dose live attenuated chimeric vaccine candidate against Zika virus
title A single-dose live attenuated chimeric vaccine candidate against Zika virus
title_full A single-dose live attenuated chimeric vaccine candidate against Zika virus
title_fullStr A single-dose live attenuated chimeric vaccine candidate against Zika virus
title_full_unstemmed A single-dose live attenuated chimeric vaccine candidate against Zika virus
title_short A single-dose live attenuated chimeric vaccine candidate against Zika virus
title_sort single-dose live attenuated chimeric vaccine candidate against zika virus
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7846741/
https://www.ncbi.nlm.nih.gov/pubmed/33514743
http://dx.doi.org/10.1038/s41541-021-00282-y
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