Serum-free adapted Drosophila S2R+ line is amenable to RNA interference

We have previously adapted a select number of Drosophila cell lines to grow in serum-free media supplemented with fly extract. This condition is arguably more representative of a native growth environment. Here, we validated that the fly extract adapted line, S2R+ (FEx 2.5%) is amenable to RNAi. RNA...

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Detalles Bibliográficos
Autores principales: Luhur, Arthur, Mariyappa, Daniel, Klueg, Kristin M, Rogers, Stephen L, Zelhof, Andrew C
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Caltech Library 2021
Materias:
Materials and Reagents
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7846936/
https://www.ncbi.nlm.nih.gov/pubmed/33537561
http://dx.doi.org/10.17912/micropub.biology.000362
Descripción
Sumario:We have previously adapted a select number of Drosophila cell lines to grow in serum-free media supplemented with fly extract. This condition is arguably more representative of a native growth environment. Here, we validated that the fly extract adapted line, S2R+ (FEx 2.5%) is amenable to RNAi. RNAi against Rho1 in both S2R+ and S2R+ (FEx 2.5%) produced phenotypes similar to ones previously described in Drosophila S2 cells.

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