Serum-free adapted Drosophila S2R+ line is amenable to RNA interference

We have previously adapted a select number of Drosophila cell lines to grow in serum-free media supplemented with fly extract. This condition is arguably more representative of a native growth environment. Here, we validated that the fly extract adapted line, S2R+ (FEx 2.5%) is amenable to RNAi. RNA...

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Detalles Bibliográficos
Autores principales: Luhur, Arthur, Mariyappa, Daniel, Klueg, Kristin M, Rogers, Stephen L, Zelhof, Andrew C
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Caltech Library 2021
Materias:
Materials and Reagents
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7846936/
https://www.ncbi.nlm.nih.gov/pubmed/33537561
http://dx.doi.org/10.17912/micropub.biology.000362
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author Luhur, Arthur
Mariyappa, Daniel
Klueg, Kristin M
Rogers, Stephen L
Zelhof, Andrew C
author_facet Luhur, Arthur
Mariyappa, Daniel
Klueg, Kristin M
Rogers, Stephen L
Zelhof, Andrew C
author_sort Luhur, Arthur
collection PubMed
description We have previously adapted a select number of Drosophila cell lines to grow in serum-free media supplemented with fly extract. This condition is arguably more representative of a native growth environment. Here, we validated that the fly extract adapted line, S2R+ (FEx 2.5%) is amenable to RNAi. RNAi against Rho1 in both S2R+ and S2R+ (FEx 2.5%) produced phenotypes similar to ones previously described in Drosophila S2 cells.
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spelling pubmed-78469362021-02-02 Serum-free adapted Drosophila S2R+ line is amenable to RNA interference Luhur, Arthur Mariyappa, Daniel Klueg, Kristin M Rogers, Stephen L Zelhof, Andrew C MicroPubl Biol Materials and Reagents We have previously adapted a select number of Drosophila cell lines to grow in serum-free media supplemented with fly extract. This condition is arguably more representative of a native growth environment. Here, we validated that the fly extract adapted line, S2R+ (FEx 2.5%) is amenable to RNAi. RNAi against Rho1 in both S2R+ and S2R+ (FEx 2.5%) produced phenotypes similar to ones previously described in Drosophila S2 cells. Caltech Library 2021-01-29 /pmc/articles/PMC7846936/ /pubmed/33537561 http://dx.doi.org/10.17912/micropub.biology.000362 Text en Copyright: © 2021 by the authors https://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Materials and Reagents
Luhur, Arthur
Mariyappa, Daniel
Klueg, Kristin M
Rogers, Stephen L
Zelhof, Andrew C
Serum-free adapted Drosophila S2R+ line is amenable to RNA interference
title Serum-free adapted Drosophila S2R+ line is amenable to RNA interference
title_full Serum-free adapted Drosophila S2R+ line is amenable to RNA interference
title_fullStr Serum-free adapted Drosophila S2R+ line is amenable to RNA interference
title_full_unstemmed Serum-free adapted Drosophila S2R+ line is amenable to RNA interference
title_short Serum-free adapted Drosophila S2R+ line is amenable to RNA interference
title_sort serum-free adapted drosophila s2r+ line is amenable to rna interference
topic Materials and Reagents
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7846936/
https://www.ncbi.nlm.nih.gov/pubmed/33537561
http://dx.doi.org/10.17912/micropub.biology.000362
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