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Extensive nuclear gyration and pervasive non-genic transcription during primordial germ cell development in zebrafish

Primordial germ cells (PGCs) are the precursors of germ cells, which migrate to the genital ridge during early development. Relatively little is known about PGCs after their migration. We studied this post-migratory stage using microscopy and sequencing techniques, and found that many PGC-specific g...

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Autores principales: Redl, Stefan, de Jesus Domingues, Antonio M., Caspani, Edoardo, Möckel, Stefanie, Salvenmoser, Willi, Mendez-Lago, Maria, Ketting, René F.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Company of Biologists Ltd 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7847270/
https://www.ncbi.nlm.nih.gov/pubmed/33298460
http://dx.doi.org/10.1242/dev.193060
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author Redl, Stefan
de Jesus Domingues, Antonio M.
Caspani, Edoardo
Möckel, Stefanie
Salvenmoser, Willi
Mendez-Lago, Maria
Ketting, René F.
author_facet Redl, Stefan
de Jesus Domingues, Antonio M.
Caspani, Edoardo
Möckel, Stefanie
Salvenmoser, Willi
Mendez-Lago, Maria
Ketting, René F.
author_sort Redl, Stefan
collection PubMed
description Primordial germ cells (PGCs) are the precursors of germ cells, which migrate to the genital ridge during early development. Relatively little is known about PGCs after their migration. We studied this post-migratory stage using microscopy and sequencing techniques, and found that many PGC-specific genes, including genes known to induce PGC fate in the mouse, are only activated several days after migration. At this same time point, PGC nuclei become extremely gyrated, displaying general broad opening of chromatin and high levels of intergenic transcription. This is accompanied by changes in nuage morphology, expression of large loci (PGC-expressed non-coding RNA loci, PERLs) that are enriched for retro-transposons and piRNAs, and a rise in piRNA biogenesis signatures. Interestingly, no nuclear Piwi protein could be detected at any time point, indicating that the zebrafish piRNA pathway is fully cytoplasmic. Our data show that the post-migratory stage of zebrafish PGCs holds many cues to both germ cell fate establishment and piRNA pathway activation.
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spelling pubmed-78472702021-02-08 Extensive nuclear gyration and pervasive non-genic transcription during primordial germ cell development in zebrafish Redl, Stefan de Jesus Domingues, Antonio M. Caspani, Edoardo Möckel, Stefanie Salvenmoser, Willi Mendez-Lago, Maria Ketting, René F. Development Research Article Primordial germ cells (PGCs) are the precursors of germ cells, which migrate to the genital ridge during early development. Relatively little is known about PGCs after their migration. We studied this post-migratory stage using microscopy and sequencing techniques, and found that many PGC-specific genes, including genes known to induce PGC fate in the mouse, are only activated several days after migration. At this same time point, PGC nuclei become extremely gyrated, displaying general broad opening of chromatin and high levels of intergenic transcription. This is accompanied by changes in nuage morphology, expression of large loci (PGC-expressed non-coding RNA loci, PERLs) that are enriched for retro-transposons and piRNAs, and a rise in piRNA biogenesis signatures. Interestingly, no nuclear Piwi protein could be detected at any time point, indicating that the zebrafish piRNA pathway is fully cytoplasmic. Our data show that the post-migratory stage of zebrafish PGCs holds many cues to both germ cell fate establishment and piRNA pathway activation. The Company of Biologists Ltd 2021-01-20 /pmc/articles/PMC7847270/ /pubmed/33298460 http://dx.doi.org/10.1242/dev.193060 Text en © 2021. Published by The Company of Biologists Ltd http://creativecommons.org/licenses/by/4.0This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.
spellingShingle Research Article
Redl, Stefan
de Jesus Domingues, Antonio M.
Caspani, Edoardo
Möckel, Stefanie
Salvenmoser, Willi
Mendez-Lago, Maria
Ketting, René F.
Extensive nuclear gyration and pervasive non-genic transcription during primordial germ cell development in zebrafish
title Extensive nuclear gyration and pervasive non-genic transcription during primordial germ cell development in zebrafish
title_full Extensive nuclear gyration and pervasive non-genic transcription during primordial germ cell development in zebrafish
title_fullStr Extensive nuclear gyration and pervasive non-genic transcription during primordial germ cell development in zebrafish
title_full_unstemmed Extensive nuclear gyration and pervasive non-genic transcription during primordial germ cell development in zebrafish
title_short Extensive nuclear gyration and pervasive non-genic transcription during primordial germ cell development in zebrafish
title_sort extensive nuclear gyration and pervasive non-genic transcription during primordial germ cell development in zebrafish
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7847270/
https://www.ncbi.nlm.nih.gov/pubmed/33298460
http://dx.doi.org/10.1242/dev.193060
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