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Highly redundant neuropeptide volume co-transmission underlying episodic activation of the GnRH neuron dendron

The necessity and functional significance of neurotransmitter co-transmission remains unclear. The glutamatergic ‘KNDy’ neurons co-express kisspeptin, neurokinin B (NKB), and dynorphin and exhibit a highly stereotyped synchronized behavior that reads out to the gonadotropin-releasing hormone (GnRH)...

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Autores principales: Liu, Xinhuai, Yeo, Shel-Hwa, McQuillan, H James, Herde, Michel K, Hessler, Sabine, Cheong, Isaiah, Porteous, Robert, Herbison, Allan E
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7847305/
https://www.ncbi.nlm.nih.gov/pubmed/33464205
http://dx.doi.org/10.7554/eLife.62455
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author Liu, Xinhuai
Yeo, Shel-Hwa
McQuillan, H James
Herde, Michel K
Hessler, Sabine
Cheong, Isaiah
Porteous, Robert
Herbison, Allan E
author_facet Liu, Xinhuai
Yeo, Shel-Hwa
McQuillan, H James
Herde, Michel K
Hessler, Sabine
Cheong, Isaiah
Porteous, Robert
Herbison, Allan E
author_sort Liu, Xinhuai
collection PubMed
description The necessity and functional significance of neurotransmitter co-transmission remains unclear. The glutamatergic ‘KNDy’ neurons co-express kisspeptin, neurokinin B (NKB), and dynorphin and exhibit a highly stereotyped synchronized behavior that reads out to the gonadotropin-releasing hormone (GnRH) neuron dendrons to drive episodic hormone secretion. Using expansion microscopy, we show that KNDy neurons make abundant close, non-synaptic appositions with the GnRH neuron dendron. Electrophysiology and confocal GCaMP6 imaging demonstrated that, despite all three neuropeptides being released from KNDy terminals, only kisspeptin was able to activate the GnRH neuron dendron. Mice with a selective deletion of kisspeptin from KNDy neurons failed to exhibit pulsatile hormone secretion but maintained synchronized episodic KNDy neuron behavior that is thought to depend on recurrent NKB and dynorphin transmission. This indicates that KNDy neurons drive episodic hormone secretion through highly redundant neuropeptide co-transmission orchestrated by differential post-synaptic neuropeptide receptor expression at the GnRH neuron dendron and KNDy neuron.
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spelling pubmed-78473052021-02-01 Highly redundant neuropeptide volume co-transmission underlying episodic activation of the GnRH neuron dendron Liu, Xinhuai Yeo, Shel-Hwa McQuillan, H James Herde, Michel K Hessler, Sabine Cheong, Isaiah Porteous, Robert Herbison, Allan E eLife Neuroscience The necessity and functional significance of neurotransmitter co-transmission remains unclear. The glutamatergic ‘KNDy’ neurons co-express kisspeptin, neurokinin B (NKB), and dynorphin and exhibit a highly stereotyped synchronized behavior that reads out to the gonadotropin-releasing hormone (GnRH) neuron dendrons to drive episodic hormone secretion. Using expansion microscopy, we show that KNDy neurons make abundant close, non-synaptic appositions with the GnRH neuron dendron. Electrophysiology and confocal GCaMP6 imaging demonstrated that, despite all three neuropeptides being released from KNDy terminals, only kisspeptin was able to activate the GnRH neuron dendron. Mice with a selective deletion of kisspeptin from KNDy neurons failed to exhibit pulsatile hormone secretion but maintained synchronized episodic KNDy neuron behavior that is thought to depend on recurrent NKB and dynorphin transmission. This indicates that KNDy neurons drive episodic hormone secretion through highly redundant neuropeptide co-transmission orchestrated by differential post-synaptic neuropeptide receptor expression at the GnRH neuron dendron and KNDy neuron. eLife Sciences Publications, Ltd 2021-01-19 /pmc/articles/PMC7847305/ /pubmed/33464205 http://dx.doi.org/10.7554/eLife.62455 Text en © 2021, Liu et al http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Neuroscience
Liu, Xinhuai
Yeo, Shel-Hwa
McQuillan, H James
Herde, Michel K
Hessler, Sabine
Cheong, Isaiah
Porteous, Robert
Herbison, Allan E
Highly redundant neuropeptide volume co-transmission underlying episodic activation of the GnRH neuron dendron
title Highly redundant neuropeptide volume co-transmission underlying episodic activation of the GnRH neuron dendron
title_full Highly redundant neuropeptide volume co-transmission underlying episodic activation of the GnRH neuron dendron
title_fullStr Highly redundant neuropeptide volume co-transmission underlying episodic activation of the GnRH neuron dendron
title_full_unstemmed Highly redundant neuropeptide volume co-transmission underlying episodic activation of the GnRH neuron dendron
title_short Highly redundant neuropeptide volume co-transmission underlying episodic activation of the GnRH neuron dendron
title_sort highly redundant neuropeptide volume co-transmission underlying episodic activation of the gnrh neuron dendron
topic Neuroscience
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7847305/
https://www.ncbi.nlm.nih.gov/pubmed/33464205
http://dx.doi.org/10.7554/eLife.62455
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