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Biological effects of electron beam to target turning X-ray (EBTTX) on two freesia (Freesia hybrida) cultivars
Electron beam to target turning X-ray (EBTTX) is an emerging irradiation technology that can potentially accelerate the breeding process of plants. The biological effects of EBTTX irradiation on the two freesia cultivars (the red freesia and the purple freesia) were investigated by establishing an i...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
PeerJ Inc.
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7847710/ https://www.ncbi.nlm.nih.gov/pubmed/33575130 http://dx.doi.org/10.7717/peerj.10742 |
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author | Li, Yi-rui Liu, Ling Wang, Dan Chen, Li Chen, Hao |
author_facet | Li, Yi-rui Liu, Ling Wang, Dan Chen, Li Chen, Hao |
author_sort | Li, Yi-rui |
collection | PubMed |
description | Electron beam to target turning X-ray (EBTTX) is an emerging irradiation technology that can potentially accelerate the breeding process of plants. The biological effects of EBTTX irradiation on the two freesia cultivars (the red freesia and the purple freesia) were investigated by establishing an irradiation-mediated mutation breeding protocol. The germination rate, survival rate, plant height, leaf number and area, root number and length of the two freesia cultivars decreased following different irradiation doses (25, 50, 75, and 100-Gy). A high irradiation dose exhibited stronger inhibition effects on these plant growth parameters, and the survival rate of the two freesia cultivars was 0.00% following the 100-Gy irradiation treatment. The median lethal dose (LD(50)) based on survival rates was 54.28-Gy for the red freesia and 60.11-Gy for the purple freesia. The flowering rate, flower number, and pollen vigor were significantly decreased by irradiation treatment. At 75-Gy irradiation, the flowering rate, flower number and pollen viability of the two varieties reached the minimum, exhibiting strong inhibitory effects. Meanwhile, 75-Gy irradiation significantly decreased the chlorophyll content and increased the malondialdehyde (MDA) content of the two freesia cultivars. Furthermore, as the irradiation dose increased, the changes in the micro-morphology of the leaf epidermis and pollen gradually increased according to a scanning electron microscope (SEM) analysis. These results are expected to provide useful information for the mutation breeding of different freesia cultivars and other flowering plants. |
format | Online Article Text |
id | pubmed-7847710 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | PeerJ Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-78477102021-02-10 Biological effects of electron beam to target turning X-ray (EBTTX) on two freesia (Freesia hybrida) cultivars Li, Yi-rui Liu, Ling Wang, Dan Chen, Li Chen, Hao PeerJ Agricultural Science Electron beam to target turning X-ray (EBTTX) is an emerging irradiation technology that can potentially accelerate the breeding process of plants. The biological effects of EBTTX irradiation on the two freesia cultivars (the red freesia and the purple freesia) were investigated by establishing an irradiation-mediated mutation breeding protocol. The germination rate, survival rate, plant height, leaf number and area, root number and length of the two freesia cultivars decreased following different irradiation doses (25, 50, 75, and 100-Gy). A high irradiation dose exhibited stronger inhibition effects on these plant growth parameters, and the survival rate of the two freesia cultivars was 0.00% following the 100-Gy irradiation treatment. The median lethal dose (LD(50)) based on survival rates was 54.28-Gy for the red freesia and 60.11-Gy for the purple freesia. The flowering rate, flower number, and pollen vigor were significantly decreased by irradiation treatment. At 75-Gy irradiation, the flowering rate, flower number and pollen viability of the two varieties reached the minimum, exhibiting strong inhibitory effects. Meanwhile, 75-Gy irradiation significantly decreased the chlorophyll content and increased the malondialdehyde (MDA) content of the two freesia cultivars. Furthermore, as the irradiation dose increased, the changes in the micro-morphology of the leaf epidermis and pollen gradually increased according to a scanning electron microscope (SEM) analysis. These results are expected to provide useful information for the mutation breeding of different freesia cultivars and other flowering plants. PeerJ Inc. 2021-01-28 /pmc/articles/PMC7847710/ /pubmed/33575130 http://dx.doi.org/10.7717/peerj.10742 Text en ©2021 Li et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited. |
spellingShingle | Agricultural Science Li, Yi-rui Liu, Ling Wang, Dan Chen, Li Chen, Hao Biological effects of electron beam to target turning X-ray (EBTTX) on two freesia (Freesia hybrida) cultivars |
title | Biological effects of electron beam to target turning X-ray (EBTTX) on two freesia (Freesia hybrida) cultivars |
title_full | Biological effects of electron beam to target turning X-ray (EBTTX) on two freesia (Freesia hybrida) cultivars |
title_fullStr | Biological effects of electron beam to target turning X-ray (EBTTX) on two freesia (Freesia hybrida) cultivars |
title_full_unstemmed | Biological effects of electron beam to target turning X-ray (EBTTX) on two freesia (Freesia hybrida) cultivars |
title_short | Biological effects of electron beam to target turning X-ray (EBTTX) on two freesia (Freesia hybrida) cultivars |
title_sort | biological effects of electron beam to target turning x-ray (ebttx) on two freesia (freesia hybrida) cultivars |
topic | Agricultural Science |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7847710/ https://www.ncbi.nlm.nih.gov/pubmed/33575130 http://dx.doi.org/10.7717/peerj.10742 |
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