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A Customizable Low-Cost System for Massively Parallel Zebrafish Behavioral Phenotyping

High-throughput behavioral phenotyping is critical to genetic or chemical screening approaches. Zebrafish larvae are amenable to high-throughput behavioral screening because of their rapid development, small size, and conserved vertebrate brain architecture. Existing commercial behavioral phenotypin...

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Autores principales: Joo, William, Vivian, Michael D., Graham, Brett J., Soucy, Edward R., Thyme, Summer B.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7847893/
https://www.ncbi.nlm.nih.gov/pubmed/33536882
http://dx.doi.org/10.3389/fnbeh.2020.606900
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author Joo, William
Vivian, Michael D.
Graham, Brett J.
Soucy, Edward R.
Thyme, Summer B.
author_facet Joo, William
Vivian, Michael D.
Graham, Brett J.
Soucy, Edward R.
Thyme, Summer B.
author_sort Joo, William
collection PubMed
description High-throughput behavioral phenotyping is critical to genetic or chemical screening approaches. Zebrafish larvae are amenable to high-throughput behavioral screening because of their rapid development, small size, and conserved vertebrate brain architecture. Existing commercial behavioral phenotyping systems are expensive and not easily modified for new assays. Here, we describe a modular, highly adaptable, and low-cost system. Along with detailed assembly and operation instructions, we provide data acquisition software and a robust, parallel analysis pipeline. We validate our approach by analyzing stimulus response profiles in larval zebrafish, confirming prepulse inhibition phenotypes of two previously isolated mutants, and highlighting best practices for growing larvae prior to behavioral testing. Our new design thus allows rapid construction and streamlined operation of many large-scale behavioral setups with minimal resources and fabrication expertise, with broad applications to other aquatic organisms.
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spelling pubmed-78478932021-02-02 A Customizable Low-Cost System for Massively Parallel Zebrafish Behavioral Phenotyping Joo, William Vivian, Michael D. Graham, Brett J. Soucy, Edward R. Thyme, Summer B. Front Behav Neurosci Behavioral Neuroscience High-throughput behavioral phenotyping is critical to genetic or chemical screening approaches. Zebrafish larvae are amenable to high-throughput behavioral screening because of their rapid development, small size, and conserved vertebrate brain architecture. Existing commercial behavioral phenotyping systems are expensive and not easily modified for new assays. Here, we describe a modular, highly adaptable, and low-cost system. Along with detailed assembly and operation instructions, we provide data acquisition software and a robust, parallel analysis pipeline. We validate our approach by analyzing stimulus response profiles in larval zebrafish, confirming prepulse inhibition phenotypes of two previously isolated mutants, and highlighting best practices for growing larvae prior to behavioral testing. Our new design thus allows rapid construction and streamlined operation of many large-scale behavioral setups with minimal resources and fabrication expertise, with broad applications to other aquatic organisms. Frontiers Media S.A. 2021-01-18 /pmc/articles/PMC7847893/ /pubmed/33536882 http://dx.doi.org/10.3389/fnbeh.2020.606900 Text en Copyright © 2021 Joo, Vivian, Graham, Soucy and Thyme. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Behavioral Neuroscience
Joo, William
Vivian, Michael D.
Graham, Brett J.
Soucy, Edward R.
Thyme, Summer B.
A Customizable Low-Cost System for Massively Parallel Zebrafish Behavioral Phenotyping
title A Customizable Low-Cost System for Massively Parallel Zebrafish Behavioral Phenotyping
title_full A Customizable Low-Cost System for Massively Parallel Zebrafish Behavioral Phenotyping
title_fullStr A Customizable Low-Cost System for Massively Parallel Zebrafish Behavioral Phenotyping
title_full_unstemmed A Customizable Low-Cost System for Massively Parallel Zebrafish Behavioral Phenotyping
title_short A Customizable Low-Cost System for Massively Parallel Zebrafish Behavioral Phenotyping
title_sort customizable low-cost system for massively parallel zebrafish behavioral phenotyping
topic Behavioral Neuroscience
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7847893/
https://www.ncbi.nlm.nih.gov/pubmed/33536882
http://dx.doi.org/10.3389/fnbeh.2020.606900
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