Deletion of BST2 Cytoplasmic and Transmembrane N-Terminal Domains Results in SARS-CoV, SARS-CoV-2, and Influenza Virus Production Suppression in a Vero Cell Line

SARS-CoV-2, which emerged in Wuhan (China), has become a great worldwide problem in 2020 and has led to more than 1,000,000 deaths worldwide. Many laboratories are searching for ways to fight this pandemic. We studied the action of the cellular antiviral protein tetherin, which is encoded by the BST...

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Autores principales: Dolskiy, Alexander A., Bodnev, Sergei A., Nazarenko, Anastasia A., Smirnova, Anastasia M., Pyankova, Olga G., Matveeva, Anna K., Grishchenko, Irina V., Tregubchak, Tatiana V., Pyankov, Oleg V., Ryzhikov, Alexander B., Gavrilova, Elena V., Maksyutov, Rinat A., Yudkin, Dmitry V.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2020
Materias:
Molecular Biosciences
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7847895/
https://www.ncbi.nlm.nih.gov/pubmed/33537341
http://dx.doi.org/10.3389/fmolb.2020.616798
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author Dolskiy, Alexander A.
Bodnev, Sergei A.
Nazarenko, Anastasia A.
Smirnova, Anastasia M.
Pyankova, Olga G.
Matveeva, Anna K.
Grishchenko, Irina V.
Tregubchak, Tatiana V.
Pyankov, Oleg V.
Ryzhikov, Alexander B.
Gavrilova, Elena V.
Maksyutov, Rinat A.
Yudkin, Dmitry V.
author_facet Dolskiy, Alexander A.
Bodnev, Sergei A.
Nazarenko, Anastasia A.
Smirnova, Anastasia M.
Pyankova, Olga G.
Matveeva, Anna K.
Grishchenko, Irina V.
Tregubchak, Tatiana V.
Pyankov, Oleg V.
Ryzhikov, Alexander B.
Gavrilova, Elena V.
Maksyutov, Rinat A.
Yudkin, Dmitry V.
author_sort Dolskiy, Alexander A.
collection PubMed
description SARS-CoV-2, which emerged in Wuhan (China), has become a great worldwide problem in 2020 and has led to more than 1,000,000 deaths worldwide. Many laboratories are searching for ways to fight this pandemic. We studied the action of the cellular antiviral protein tetherin, which is encoded by the BST2 gene. We deleted the transmembrane domain-encoding part of the gene in the Vero cell line. The transmembrane domain is a target for virus-antagonizing proteins. We showed a decrease in SARS-CoV-2 in cells with deleted transmembrane BST2 domains compared to the initial Vero cell line. Similar results were obtained for SARS-CoV and avian influenza virus. This finding may help the development of antiviral therapies competitively targeting the transmembrane domain of tetherin with viral-antagonizing proteins.
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spelling pubmed-78478952021-02-02 Deletion of BST2 Cytoplasmic and Transmembrane N-Terminal Domains Results in SARS-CoV, SARS-CoV-2, and Influenza Virus Production Suppression in a Vero Cell Line Dolskiy, Alexander A. Bodnev, Sergei A. Nazarenko, Anastasia A. Smirnova, Anastasia M. Pyankova, Olga G. Matveeva, Anna K. Grishchenko, Irina V. Tregubchak, Tatiana V. Pyankov, Oleg V. Ryzhikov, Alexander B. Gavrilova, Elena V. Maksyutov, Rinat A. Yudkin, Dmitry V. Front Mol Biosci Molecular Biosciences SARS-CoV-2, which emerged in Wuhan (China), has become a great worldwide problem in 2020 and has led to more than 1,000,000 deaths worldwide. Many laboratories are searching for ways to fight this pandemic. We studied the action of the cellular antiviral protein tetherin, which is encoded by the BST2 gene. We deleted the transmembrane domain-encoding part of the gene in the Vero cell line. The transmembrane domain is a target for virus-antagonizing proteins. We showed a decrease in SARS-CoV-2 in cells with deleted transmembrane BST2 domains compared to the initial Vero cell line. Similar results were obtained for SARS-CoV and avian influenza virus. This finding may help the development of antiviral therapies competitively targeting the transmembrane domain of tetherin with viral-antagonizing proteins. Frontiers Media S.A. 2020-12-18 /pmc/articles/PMC7847895/ /pubmed/33537341 http://dx.doi.org/10.3389/fmolb.2020.616798 Text en Copyright © 2020 Dolskiy, Bodnev, Nazarenko, Smirnova, Pyankova, Matveeva, Grishchenko, Tregubchak, Pyankov, Ryzhikov, Gavrilova, Maksyutov and Yudkin. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Molecular Biosciences
Dolskiy, Alexander A.
Bodnev, Sergei A.
Nazarenko, Anastasia A.
Smirnova, Anastasia M.
Pyankova, Olga G.
Matveeva, Anna K.
Grishchenko, Irina V.
Tregubchak, Tatiana V.
Pyankov, Oleg V.
Ryzhikov, Alexander B.
Gavrilova, Elena V.
Maksyutov, Rinat A.
Yudkin, Dmitry V.
Deletion of BST2 Cytoplasmic and Transmembrane N-Terminal Domains Results in SARS-CoV, SARS-CoV-2, and Influenza Virus Production Suppression in a Vero Cell Line
title Deletion of BST2 Cytoplasmic and Transmembrane N-Terminal Domains Results in SARS-CoV, SARS-CoV-2, and Influenza Virus Production Suppression in a Vero Cell Line
title_full Deletion of BST2 Cytoplasmic and Transmembrane N-Terminal Domains Results in SARS-CoV, SARS-CoV-2, and Influenza Virus Production Suppression in a Vero Cell Line
title_fullStr Deletion of BST2 Cytoplasmic and Transmembrane N-Terminal Domains Results in SARS-CoV, SARS-CoV-2, and Influenza Virus Production Suppression in a Vero Cell Line
title_full_unstemmed Deletion of BST2 Cytoplasmic and Transmembrane N-Terminal Domains Results in SARS-CoV, SARS-CoV-2, and Influenza Virus Production Suppression in a Vero Cell Line
title_short Deletion of BST2 Cytoplasmic and Transmembrane N-Terminal Domains Results in SARS-CoV, SARS-CoV-2, and Influenza Virus Production Suppression in a Vero Cell Line
title_sort deletion of bst2 cytoplasmic and transmembrane n-terminal domains results in sars-cov, sars-cov-2, and influenza virus production suppression in a vero cell line
topic Molecular Biosciences
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7847895/
https://www.ncbi.nlm.nih.gov/pubmed/33537341
http://dx.doi.org/10.3389/fmolb.2020.616798
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