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Etiology of Cyclocarya paliurus Anthracnose in Jiangsu Province, China

Cyclocarya paliurus is an extremely valuable and multifunctional tree species whose leaves have traditionally been used in used in medicine or as a medicinal tea in China. In recent years, anthracnose has been frequently observed on young leaves of C. paliurus in several nurseries located in Jiangsu...

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Autores principales: Zheng, Xiang-rong, Zhang, Mao-jiao, Shang, Xu-lan, Fang, Sheng-zuo, Chen, Feng-mao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7847979/
https://www.ncbi.nlm.nih.gov/pubmed/33537048
http://dx.doi.org/10.3389/fpls.2020.613499
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author Zheng, Xiang-rong
Zhang, Mao-jiao
Shang, Xu-lan
Fang, Sheng-zuo
Chen, Feng-mao
author_facet Zheng, Xiang-rong
Zhang, Mao-jiao
Shang, Xu-lan
Fang, Sheng-zuo
Chen, Feng-mao
author_sort Zheng, Xiang-rong
collection PubMed
description Cyclocarya paliurus is an extremely valuable and multifunctional tree species whose leaves have traditionally been used in used in medicine or as a medicinal tea in China. In recent years, anthracnose has been frequently observed on young leaves of C. paliurus in several nurseries located in Jiangsu Province, resulting in great yield and quality losses. To date, no information is available about the prevalence of C. paliurus anthracnose in China. The main purpose of the present study was to characterize the etiology of C. paliurus anthracnose. Phylogenetic analysis of the eight-loci concatenated dataset revealed that all 44 single-spore Colletotrichum isolates belonged to three species in the Colletotrichum gloeosporioides species complex, namely, Colletotrichum aenigma, Colletotrichum fructicola, and C. gloeosporioides sensu stricto. Phenotypic features, including the colony appearance and the morphology of conidia, appressoria, and ascospores, were consistent with the phylogenetic grouping. Virulence tests validated that the three Colletotrichum species could cause typical symptoms of anthracnose on C. paliurus leaves, similar to those observed in the field. The optimum mycelial growth temperature ranged from 25 to 30°C for all representative isolates, while C. gloeosporioides s. s. isolates exhibited greater tolerance to high temperature (40°C). Fungicide sensitivity assays indicated that all three Colletotrichum species were sensitive to tetramycin, which may be a potential alternative for the management of C. paliurus anthracnose. To our knowledge, this study provides the first report of C. aenigma, C. fructicola, and C. gloeosporioides s. s. causing C. paliurus anthracnose in China as well as in the world.
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spelling pubmed-78479792021-02-02 Etiology of Cyclocarya paliurus Anthracnose in Jiangsu Province, China Zheng, Xiang-rong Zhang, Mao-jiao Shang, Xu-lan Fang, Sheng-zuo Chen, Feng-mao Front Plant Sci Plant Science Cyclocarya paliurus is an extremely valuable and multifunctional tree species whose leaves have traditionally been used in used in medicine or as a medicinal tea in China. In recent years, anthracnose has been frequently observed on young leaves of C. paliurus in several nurseries located in Jiangsu Province, resulting in great yield and quality losses. To date, no information is available about the prevalence of C. paliurus anthracnose in China. The main purpose of the present study was to characterize the etiology of C. paliurus anthracnose. Phylogenetic analysis of the eight-loci concatenated dataset revealed that all 44 single-spore Colletotrichum isolates belonged to three species in the Colletotrichum gloeosporioides species complex, namely, Colletotrichum aenigma, Colletotrichum fructicola, and C. gloeosporioides sensu stricto. Phenotypic features, including the colony appearance and the morphology of conidia, appressoria, and ascospores, were consistent with the phylogenetic grouping. Virulence tests validated that the three Colletotrichum species could cause typical symptoms of anthracnose on C. paliurus leaves, similar to those observed in the field. The optimum mycelial growth temperature ranged from 25 to 30°C for all representative isolates, while C. gloeosporioides s. s. isolates exhibited greater tolerance to high temperature (40°C). Fungicide sensitivity assays indicated that all three Colletotrichum species were sensitive to tetramycin, which may be a potential alternative for the management of C. paliurus anthracnose. To our knowledge, this study provides the first report of C. aenigma, C. fructicola, and C. gloeosporioides s. s. causing C. paliurus anthracnose in China as well as in the world. Frontiers Media S.A. 2021-01-18 /pmc/articles/PMC7847979/ /pubmed/33537048 http://dx.doi.org/10.3389/fpls.2020.613499 Text en Copyright © 2021 Zheng, Zhang, Shang, Fang and Chen. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Zheng, Xiang-rong
Zhang, Mao-jiao
Shang, Xu-lan
Fang, Sheng-zuo
Chen, Feng-mao
Etiology of Cyclocarya paliurus Anthracnose in Jiangsu Province, China
title Etiology of Cyclocarya paliurus Anthracnose in Jiangsu Province, China
title_full Etiology of Cyclocarya paliurus Anthracnose in Jiangsu Province, China
title_fullStr Etiology of Cyclocarya paliurus Anthracnose in Jiangsu Province, China
title_full_unstemmed Etiology of Cyclocarya paliurus Anthracnose in Jiangsu Province, China
title_short Etiology of Cyclocarya paliurus Anthracnose in Jiangsu Province, China
title_sort etiology of cyclocarya paliurus anthracnose in jiangsu province, china
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7847979/
https://www.ncbi.nlm.nih.gov/pubmed/33537048
http://dx.doi.org/10.3389/fpls.2020.613499
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