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Characterization of the Biodistribution of a Silica Vesicle Nanovaccine Carrying a Rhipicephalus (Boophilus) microplus Protective Antigen With in vivo Live Animal Imaging

Development of veterinary subunit vaccines comes with a spectrum of challenges, such as the choice of adjuvant, antigen delivery vehicle, and optimization of dosing strategy. Over the years, our laboratory has largely focused on investigating silica vesicles (SVs) for developing effective veterinary...

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Autores principales: Mody, Karishma T., Zhang, Bing, Li, Xun, Fletcher, Nicholas L., Akhter, Dewan T., Jarrett, Sandy, Zhang, Jun, Yu, Chengzhong, Thurecht, Kristofer J., Mahony, Timothy J., Mitter, Neena
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7848120/
https://www.ncbi.nlm.nih.gov/pubmed/33537291
http://dx.doi.org/10.3389/fbioe.2020.606652
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author Mody, Karishma T.
Zhang, Bing
Li, Xun
Fletcher, Nicholas L.
Akhter, Dewan T.
Jarrett, Sandy
Zhang, Jun
Yu, Chengzhong
Thurecht, Kristofer J.
Mahony, Timothy J.
Mitter, Neena
author_facet Mody, Karishma T.
Zhang, Bing
Li, Xun
Fletcher, Nicholas L.
Akhter, Dewan T.
Jarrett, Sandy
Zhang, Jun
Yu, Chengzhong
Thurecht, Kristofer J.
Mahony, Timothy J.
Mitter, Neena
author_sort Mody, Karishma T.
collection PubMed
description Development of veterinary subunit vaccines comes with a spectrum of challenges, such as the choice of adjuvant, antigen delivery vehicle, and optimization of dosing strategy. Over the years, our laboratory has largely focused on investigating silica vesicles (SVs) for developing effective veterinary vaccines for multiple targets. Rhipicephalus microplus (cattle tick) are known to have a high impact on cattle health and the livestock industry in the tropical and subtropical regions. Development of vaccine using Bm86 antigen against R. microplus has emerged as an attractive alternative to control ticks. In this study, we have investigated the biodistribution of SV in a live animal model, as well as further explored the SV ability for vaccine development. Rhodamine-labeled SV-140-C(18) (Rho-SV-140-C(18)) vesicles were used to adsorb the Cy5-labeled R. microplus Bm86 antigen (Cy5-Bm86) to enable detection and characterization of the biodistribution of SV as well as antigen in vivo in a small animal model for up to 28 days using optical fluorescence imaging. We tracked the in vivo biodistribution of SVs and Bm86 antigen at different timepoints (days 3, 8, 13, and 28) in BALB/c mice. The biodistribution analysis by live imaging as well as by measuring the fluorescent intensity of harvested organs over the duration of the experiment (28 days) showed greater accumulation of SVs at the site of injection. The Bm86 antigen biodistribution was traced in lymph nodes, kidney, and liver, contributing to our understanding how this delivery platform successfully elicits antibody responses in the groups administered antigen in combination with SV. Selected tissues (skin, lymph nodes, spleen, kidney, liver, and lungs) were examined for any cellular abnormalities by histological analysis. No adverse effect or any other abnormalities were observed in the tissues.
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spelling pubmed-78481202021-02-02 Characterization of the Biodistribution of a Silica Vesicle Nanovaccine Carrying a Rhipicephalus (Boophilus) microplus Protective Antigen With in vivo Live Animal Imaging Mody, Karishma T. Zhang, Bing Li, Xun Fletcher, Nicholas L. Akhter, Dewan T. Jarrett, Sandy Zhang, Jun Yu, Chengzhong Thurecht, Kristofer J. Mahony, Timothy J. Mitter, Neena Front Bioeng Biotechnol Bioengineering and Biotechnology Development of veterinary subunit vaccines comes with a spectrum of challenges, such as the choice of adjuvant, antigen delivery vehicle, and optimization of dosing strategy. Over the years, our laboratory has largely focused on investigating silica vesicles (SVs) for developing effective veterinary vaccines for multiple targets. Rhipicephalus microplus (cattle tick) are known to have a high impact on cattle health and the livestock industry in the tropical and subtropical regions. Development of vaccine using Bm86 antigen against R. microplus has emerged as an attractive alternative to control ticks. In this study, we have investigated the biodistribution of SV in a live animal model, as well as further explored the SV ability for vaccine development. Rhodamine-labeled SV-140-C(18) (Rho-SV-140-C(18)) vesicles were used to adsorb the Cy5-labeled R. microplus Bm86 antigen (Cy5-Bm86) to enable detection and characterization of the biodistribution of SV as well as antigen in vivo in a small animal model for up to 28 days using optical fluorescence imaging. We tracked the in vivo biodistribution of SVs and Bm86 antigen at different timepoints (days 3, 8, 13, and 28) in BALB/c mice. The biodistribution analysis by live imaging as well as by measuring the fluorescent intensity of harvested organs over the duration of the experiment (28 days) showed greater accumulation of SVs at the site of injection. The Bm86 antigen biodistribution was traced in lymph nodes, kidney, and liver, contributing to our understanding how this delivery platform successfully elicits antibody responses in the groups administered antigen in combination with SV. Selected tissues (skin, lymph nodes, spleen, kidney, liver, and lungs) were examined for any cellular abnormalities by histological analysis. No adverse effect or any other abnormalities were observed in the tissues. Frontiers Media S.A. 2021-01-18 /pmc/articles/PMC7848120/ /pubmed/33537291 http://dx.doi.org/10.3389/fbioe.2020.606652 Text en Copyright © 2021 Mody, Zhang, Li, Fletcher, Akhter, Jarrett, Zhang, Yu, Thurecht, Mahony and Mitter. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Bioengineering and Biotechnology
Mody, Karishma T.
Zhang, Bing
Li, Xun
Fletcher, Nicholas L.
Akhter, Dewan T.
Jarrett, Sandy
Zhang, Jun
Yu, Chengzhong
Thurecht, Kristofer J.
Mahony, Timothy J.
Mitter, Neena
Characterization of the Biodistribution of a Silica Vesicle Nanovaccine Carrying a Rhipicephalus (Boophilus) microplus Protective Antigen With in vivo Live Animal Imaging
title Characterization of the Biodistribution of a Silica Vesicle Nanovaccine Carrying a Rhipicephalus (Boophilus) microplus Protective Antigen With in vivo Live Animal Imaging
title_full Characterization of the Biodistribution of a Silica Vesicle Nanovaccine Carrying a Rhipicephalus (Boophilus) microplus Protective Antigen With in vivo Live Animal Imaging
title_fullStr Characterization of the Biodistribution of a Silica Vesicle Nanovaccine Carrying a Rhipicephalus (Boophilus) microplus Protective Antigen With in vivo Live Animal Imaging
title_full_unstemmed Characterization of the Biodistribution of a Silica Vesicle Nanovaccine Carrying a Rhipicephalus (Boophilus) microplus Protective Antigen With in vivo Live Animal Imaging
title_short Characterization of the Biodistribution of a Silica Vesicle Nanovaccine Carrying a Rhipicephalus (Boophilus) microplus Protective Antigen With in vivo Live Animal Imaging
title_sort characterization of the biodistribution of a silica vesicle nanovaccine carrying a rhipicephalus (boophilus) microplus protective antigen with in vivo live animal imaging
topic Bioengineering and Biotechnology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7848120/
https://www.ncbi.nlm.nih.gov/pubmed/33537291
http://dx.doi.org/10.3389/fbioe.2020.606652
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