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CRISPR-Cas13-Mediated Knockdown of lncRNA-GACAT3 Inhibited Cell Proliferation and Motility, and Induced Apoptosis by Increasing p21, Bax, and E-Cadherin Expression in Bladder Cancer

The current study is to investigate the expression pattern and biological function of long non-coding RNA Focally gastric cancer-associated transcript3 (GACAT3) in bladder cancer. Real-time quantitative qPCR was used to detect the expression level of GACAT-3 in tumor tissues and paired normal tissue...

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Autores principales: Zhang, Zhongfu, Chen, Jieqing, Zhu, Zhongshuang, Zhu, Zhongqing, Liao, Xinhui, Wu, Jianting, Cheng, Jianli, Zhang, Xintao, Mei, Hongbing, Yang, Guosheng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7848205/
https://www.ncbi.nlm.nih.gov/pubmed/33537343
http://dx.doi.org/10.3389/fmolb.2020.627774
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author Zhang, Zhongfu
Chen, Jieqing
Zhu, Zhongshuang
Zhu, Zhongqing
Liao, Xinhui
Wu, Jianting
Cheng, Jianli
Zhang, Xintao
Mei, Hongbing
Yang, Guosheng
author_facet Zhang, Zhongfu
Chen, Jieqing
Zhu, Zhongshuang
Zhu, Zhongqing
Liao, Xinhui
Wu, Jianting
Cheng, Jianli
Zhang, Xintao
Mei, Hongbing
Yang, Guosheng
author_sort Zhang, Zhongfu
collection PubMed
description The current study is to investigate the expression pattern and biological function of long non-coding RNA Focally gastric cancer-associated transcript3 (GACAT3) in bladder cancer. Real-time quantitative qPCR was used to detect the expression level of GACAT-3 in tumor tissues and paired normal tissues. Human bladder cancer T24 and 5637 cell lines were transiently transfected with specific CRISPR-Cas13 or negative control CRISPR-Cas13. Cell migration, proliferation, and apoptosis were measured by using wound healing assay CCK-8 assay and Caspase-3 ELISA assay, respectively. The expression changes of p21, Bax, and E-cadherin after knockdown of GACAT3 were detected by using Western blot. The results demonstrated that GACAT3 was up-regulated in bladder cancer tissues than that in the paired normal tissues. Inhibition of cell proliferation, increased apoptosis, and decreased motility were observed in T24 and 5637 cell lines transfected by CRISPR-Cas13 targeting GACAT3. Downregulation of GACAT3 increased p21, Bax, and E-cadherin expression and silencing these genes could eliminate the phenotypic changes induced by knockdown of GACAT3. A ceRNA mechanism for GACAT3 was also revealed. By using CRISPR-Cas13 biotechnology, we suggested that GACAT3 may be a novel target for diagnosis and treatment of bladder cancer.
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spelling pubmed-78482052021-02-02 CRISPR-Cas13-Mediated Knockdown of lncRNA-GACAT3 Inhibited Cell Proliferation and Motility, and Induced Apoptosis by Increasing p21, Bax, and E-Cadherin Expression in Bladder Cancer Zhang, Zhongfu Chen, Jieqing Zhu, Zhongshuang Zhu, Zhongqing Liao, Xinhui Wu, Jianting Cheng, Jianli Zhang, Xintao Mei, Hongbing Yang, Guosheng Front Mol Biosci Molecular Biosciences The current study is to investigate the expression pattern and biological function of long non-coding RNA Focally gastric cancer-associated transcript3 (GACAT3) in bladder cancer. Real-time quantitative qPCR was used to detect the expression level of GACAT-3 in tumor tissues and paired normal tissues. Human bladder cancer T24 and 5637 cell lines were transiently transfected with specific CRISPR-Cas13 or negative control CRISPR-Cas13. Cell migration, proliferation, and apoptosis were measured by using wound healing assay CCK-8 assay and Caspase-3 ELISA assay, respectively. The expression changes of p21, Bax, and E-cadherin after knockdown of GACAT3 were detected by using Western blot. The results demonstrated that GACAT3 was up-regulated in bladder cancer tissues than that in the paired normal tissues. Inhibition of cell proliferation, increased apoptosis, and decreased motility were observed in T24 and 5637 cell lines transfected by CRISPR-Cas13 targeting GACAT3. Downregulation of GACAT3 increased p21, Bax, and E-cadherin expression and silencing these genes could eliminate the phenotypic changes induced by knockdown of GACAT3. A ceRNA mechanism for GACAT3 was also revealed. By using CRISPR-Cas13 biotechnology, we suggested that GACAT3 may be a novel target for diagnosis and treatment of bladder cancer. Frontiers Media S.A. 2021-01-18 /pmc/articles/PMC7848205/ /pubmed/33537343 http://dx.doi.org/10.3389/fmolb.2020.627774 Text en Copyright © 2021 Zhang, Chen, Zhu, Zhu, Liao, Wu, Cheng, Zhang, Mei and Yang. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Molecular Biosciences
Zhang, Zhongfu
Chen, Jieqing
Zhu, Zhongshuang
Zhu, Zhongqing
Liao, Xinhui
Wu, Jianting
Cheng, Jianli
Zhang, Xintao
Mei, Hongbing
Yang, Guosheng
CRISPR-Cas13-Mediated Knockdown of lncRNA-GACAT3 Inhibited Cell Proliferation and Motility, and Induced Apoptosis by Increasing p21, Bax, and E-Cadherin Expression in Bladder Cancer
title CRISPR-Cas13-Mediated Knockdown of lncRNA-GACAT3 Inhibited Cell Proliferation and Motility, and Induced Apoptosis by Increasing p21, Bax, and E-Cadherin Expression in Bladder Cancer
title_full CRISPR-Cas13-Mediated Knockdown of lncRNA-GACAT3 Inhibited Cell Proliferation and Motility, and Induced Apoptosis by Increasing p21, Bax, and E-Cadherin Expression in Bladder Cancer
title_fullStr CRISPR-Cas13-Mediated Knockdown of lncRNA-GACAT3 Inhibited Cell Proliferation and Motility, and Induced Apoptosis by Increasing p21, Bax, and E-Cadherin Expression in Bladder Cancer
title_full_unstemmed CRISPR-Cas13-Mediated Knockdown of lncRNA-GACAT3 Inhibited Cell Proliferation and Motility, and Induced Apoptosis by Increasing p21, Bax, and E-Cadherin Expression in Bladder Cancer
title_short CRISPR-Cas13-Mediated Knockdown of lncRNA-GACAT3 Inhibited Cell Proliferation and Motility, and Induced Apoptosis by Increasing p21, Bax, and E-Cadherin Expression in Bladder Cancer
title_sort crispr-cas13-mediated knockdown of lncrna-gacat3 inhibited cell proliferation and motility, and induced apoptosis by increasing p21, bax, and e-cadherin expression in bladder cancer
topic Molecular Biosciences
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7848205/
https://www.ncbi.nlm.nih.gov/pubmed/33537343
http://dx.doi.org/10.3389/fmolb.2020.627774
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