Cargando…

Characterization of the intestinal mucosal proteome in cats with inflammatory bowel disease and alimentary small cell lymphoma

BACKGROUND: Current tests for diagnosis and differentiation of lymphoplasmacytic enteritis (LPE) and small cell lymphoma (SCL) in cats are expensive, invasive, and lack specificity. The identification of less invasive, more reliable biomarkers would facilitate diagnosis. OBJECTIVES: To characterize...

Descripción completa

Detalles Bibliográficos
Autores principales: Marsilio, Sina, Dröes, Floris C., Dangott, Lawrence, Chow, Betty, Hill, Steve, Ackermann, Mark, Estep, J. Scott, Lidbury, Jonathan A., Suchodolski, Jan S., Steiner, Jörg M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley & Sons, Inc. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7848303/
https://www.ncbi.nlm.nih.gov/pubmed/33471936
http://dx.doi.org/10.1111/jvim.16003
_version_ 1783645104870785024
author Marsilio, Sina
Dröes, Floris C.
Dangott, Lawrence
Chow, Betty
Hill, Steve
Ackermann, Mark
Estep, J. Scott
Lidbury, Jonathan A.
Suchodolski, Jan S.
Steiner, Jörg M.
author_facet Marsilio, Sina
Dröes, Floris C.
Dangott, Lawrence
Chow, Betty
Hill, Steve
Ackermann, Mark
Estep, J. Scott
Lidbury, Jonathan A.
Suchodolski, Jan S.
Steiner, Jörg M.
author_sort Marsilio, Sina
collection PubMed
description BACKGROUND: Current tests for diagnosis and differentiation of lymphoplasmacytic enteritis (LPE) and small cell lymphoma (SCL) in cats are expensive, invasive, and lack specificity. The identification of less invasive, more reliable biomarkers would facilitate diagnosis. OBJECTIVES: To characterize the mucosal proteome in endoscopically obtained, small intestinal tissue biopsy specimens. We hypothesized that differentially expressed proteins could be identified and serve as biomarker candidates for the differentiation of LPE and SCL in cats. ANIMALS: Six healthy control cats, 6 cats with LPE, and 8 cats with SCL. METHODS: The mucosal proteome was analyzed using 2‐dimensional fluorescence difference gel electrophoresis (2D DIGE) and nanoflow liquid chromatography tandem mass spectrometry. For 5 proteins, results were verified by Western blot analysis. RESULTS: A total of 2349 spots were identified, of which 9 were differentially expressed with a ≥2‐fold change between healthy cats and cats with LPE and SCL (.01 < P < .001). Eight of these 9 spots were also differentially expressed between cats with LPE and cats with SCL (P .001 < P < .04). However, Western blot analysis for malate dehydrogenase‐1, malate dehydrogenase‐2, apolipoprotein, annexin IV, and annexin V did not confirm significant differential protein expression for any of the 5 proteins assessed. CONCLUSIONS AND CLINICAL IMPORTANCE: Two‐D DIGE did not identify potential biomarker candidates in the intestinal mucosa of cats with LPE and SCL. Future studies should focus on different techniques to identify biomarker candidates for cats with chronic enteropathies (CE).
format Online
Article
Text
id pubmed-7848303
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher John Wiley & Sons, Inc.
record_format MEDLINE/PubMed
spelling pubmed-78483032021-02-05 Characterization of the intestinal mucosal proteome in cats with inflammatory bowel disease and alimentary small cell lymphoma Marsilio, Sina Dröes, Floris C. Dangott, Lawrence Chow, Betty Hill, Steve Ackermann, Mark Estep, J. Scott Lidbury, Jonathan A. Suchodolski, Jan S. Steiner, Jörg M. J Vet Intern Med SMALL ANIMAL BACKGROUND: Current tests for diagnosis and differentiation of lymphoplasmacytic enteritis (LPE) and small cell lymphoma (SCL) in cats are expensive, invasive, and lack specificity. The identification of less invasive, more reliable biomarkers would facilitate diagnosis. OBJECTIVES: To characterize the mucosal proteome in endoscopically obtained, small intestinal tissue biopsy specimens. We hypothesized that differentially expressed proteins could be identified and serve as biomarker candidates for the differentiation of LPE and SCL in cats. ANIMALS: Six healthy control cats, 6 cats with LPE, and 8 cats with SCL. METHODS: The mucosal proteome was analyzed using 2‐dimensional fluorescence difference gel electrophoresis (2D DIGE) and nanoflow liquid chromatography tandem mass spectrometry. For 5 proteins, results were verified by Western blot analysis. RESULTS: A total of 2349 spots were identified, of which 9 were differentially expressed with a ≥2‐fold change between healthy cats and cats with LPE and SCL (.01 < P < .001). Eight of these 9 spots were also differentially expressed between cats with LPE and cats with SCL (P .001 < P < .04). However, Western blot analysis for malate dehydrogenase‐1, malate dehydrogenase‐2, apolipoprotein, annexin IV, and annexin V did not confirm significant differential protein expression for any of the 5 proteins assessed. CONCLUSIONS AND CLINICAL IMPORTANCE: Two‐D DIGE did not identify potential biomarker candidates in the intestinal mucosa of cats with LPE and SCL. Future studies should focus on different techniques to identify biomarker candidates for cats with chronic enteropathies (CE). John Wiley & Sons, Inc. 2021-01-20 2021 /pmc/articles/PMC7848303/ /pubmed/33471936 http://dx.doi.org/10.1111/jvim.16003 Text en © 2021 The Authors. Journal of Veterinary Internal Medicine published by Wiley Periodicals LLC. on behalf of the American College of Veterinary Internal Medicine. This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.
spellingShingle SMALL ANIMAL
Marsilio, Sina
Dröes, Floris C.
Dangott, Lawrence
Chow, Betty
Hill, Steve
Ackermann, Mark
Estep, J. Scott
Lidbury, Jonathan A.
Suchodolski, Jan S.
Steiner, Jörg M.
Characterization of the intestinal mucosal proteome in cats with inflammatory bowel disease and alimentary small cell lymphoma
title Characterization of the intestinal mucosal proteome in cats with inflammatory bowel disease and alimentary small cell lymphoma
title_full Characterization of the intestinal mucosal proteome in cats with inflammatory bowel disease and alimentary small cell lymphoma
title_fullStr Characterization of the intestinal mucosal proteome in cats with inflammatory bowel disease and alimentary small cell lymphoma
title_full_unstemmed Characterization of the intestinal mucosal proteome in cats with inflammatory bowel disease and alimentary small cell lymphoma
title_short Characterization of the intestinal mucosal proteome in cats with inflammatory bowel disease and alimentary small cell lymphoma
title_sort characterization of the intestinal mucosal proteome in cats with inflammatory bowel disease and alimentary small cell lymphoma
topic SMALL ANIMAL
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7848303/
https://www.ncbi.nlm.nih.gov/pubmed/33471936
http://dx.doi.org/10.1111/jvim.16003
work_keys_str_mv AT marsiliosina characterizationoftheintestinalmucosalproteomeincatswithinflammatoryboweldiseaseandalimentarysmallcelllymphoma
AT droesflorisc characterizationoftheintestinalmucosalproteomeincatswithinflammatoryboweldiseaseandalimentarysmallcelllymphoma
AT dangottlawrence characterizationoftheintestinalmucosalproteomeincatswithinflammatoryboweldiseaseandalimentarysmallcelllymphoma
AT chowbetty characterizationoftheintestinalmucosalproteomeincatswithinflammatoryboweldiseaseandalimentarysmallcelllymphoma
AT hillsteve characterizationoftheintestinalmucosalproteomeincatswithinflammatoryboweldiseaseandalimentarysmallcelllymphoma
AT ackermannmark characterizationoftheintestinalmucosalproteomeincatswithinflammatoryboweldiseaseandalimentarysmallcelllymphoma
AT estepjscott characterizationoftheintestinalmucosalproteomeincatswithinflammatoryboweldiseaseandalimentarysmallcelllymphoma
AT lidburyjonathana characterizationoftheintestinalmucosalproteomeincatswithinflammatoryboweldiseaseandalimentarysmallcelllymphoma
AT suchodolskijans characterizationoftheintestinalmucosalproteomeincatswithinflammatoryboweldiseaseandalimentarysmallcelllymphoma
AT steinerjorgm characterizationoftheintestinalmucosalproteomeincatswithinflammatoryboweldiseaseandalimentarysmallcelllymphoma