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Long Noncoding RNA LINC01296 Harbors miR-21a to Regulate Colon Carcinoma Proliferation and Invasion
Increasing evidence has demonstrated that aberrant expressions of long noncoding RNAs (lncRNAs) are closely correlated to various malignancies, as well as colon carcinoma (CC). The aim of this study was to investigate the role of lncRNA long intergenic noncoding RNA 001296 (LINC01296) in tumorigenes...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Cognizant Communication Corporation
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7848363/ https://www.ncbi.nlm.nih.gov/pubmed/29673421 http://dx.doi.org/10.3727/096504018X15234931503876 |
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author | Wang, Kecheng Zhang, Meng Wang, Cong Ning, Xiaofei |
author_facet | Wang, Kecheng Zhang, Meng Wang, Cong Ning, Xiaofei |
author_sort | Wang, Kecheng |
collection | PubMed |
description | Increasing evidence has demonstrated that aberrant expressions of long noncoding RNAs (lncRNAs) are closely correlated to various malignancies, as well as colon carcinoma (CC). The aim of this study was to investigate the role of lncRNA long intergenic noncoding RNA 001296 (LINC01296) in tumorigenesis of CC. The result of the quantitative real-time polymerase chain reaction (qRT-PCR) demonstrated that LINC01296 was upregulated in CC cancerous tissues and cell lines compared to adjacent normal tissue and normal liver cell lines. LINC01296 overexpression was associated with poor prognosis and lower survival rate. Moreover, LINC01296 silencing inhibited the proliferation and invasion of CC cells in vitro detected by epithelial–mesenchymal transition (EMT). Bioinformatics analysis revealed that miR-21a targeted the 3′-UTR of LINC01296. Rescue experiments confirmed that miR21a could reverse the function of LINC01296 on CC cells. Together, our findings indicated that overexpression of LINC01296 is associated with poor survival of CC patients and promotes CC cell progression by regulating miR-21a, providing a prognostic biomarker and therapeutic target. |
format | Online Article Text |
id | pubmed-7848363 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Cognizant Communication Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-78483632021-02-16 Long Noncoding RNA LINC01296 Harbors miR-21a to Regulate Colon Carcinoma Proliferation and Invasion Wang, Kecheng Zhang, Meng Wang, Cong Ning, Xiaofei Oncol Res Article Increasing evidence has demonstrated that aberrant expressions of long noncoding RNAs (lncRNAs) are closely correlated to various malignancies, as well as colon carcinoma (CC). The aim of this study was to investigate the role of lncRNA long intergenic noncoding RNA 001296 (LINC01296) in tumorigenesis of CC. The result of the quantitative real-time polymerase chain reaction (qRT-PCR) demonstrated that LINC01296 was upregulated in CC cancerous tissues and cell lines compared to adjacent normal tissue and normal liver cell lines. LINC01296 overexpression was associated with poor prognosis and lower survival rate. Moreover, LINC01296 silencing inhibited the proliferation and invasion of CC cells in vitro detected by epithelial–mesenchymal transition (EMT). Bioinformatics analysis revealed that miR-21a targeted the 3′-UTR of LINC01296. Rescue experiments confirmed that miR21a could reverse the function of LINC01296 on CC cells. Together, our findings indicated that overexpression of LINC01296 is associated with poor survival of CC patients and promotes CC cell progression by regulating miR-21a, providing a prognostic biomarker and therapeutic target. Cognizant Communication Corporation 2019-05-07 /pmc/articles/PMC7848363/ /pubmed/29673421 http://dx.doi.org/10.3727/096504018X15234931503876 Text en Copyright © 2019 Cognizant, LLC. http://creativecommons.org/licenses/by-nc-nd/4.0/ This article is licensed under a Creative Commons Attribution-NonCommercial NoDerivatives 4.0 International License. |
spellingShingle | Article Wang, Kecheng Zhang, Meng Wang, Cong Ning, Xiaofei Long Noncoding RNA LINC01296 Harbors miR-21a to Regulate Colon Carcinoma Proliferation and Invasion |
title | Long Noncoding RNA LINC01296 Harbors miR-21a to Regulate Colon Carcinoma Proliferation and Invasion |
title_full | Long Noncoding RNA LINC01296 Harbors miR-21a to Regulate Colon Carcinoma Proliferation and Invasion |
title_fullStr | Long Noncoding RNA LINC01296 Harbors miR-21a to Regulate Colon Carcinoma Proliferation and Invasion |
title_full_unstemmed | Long Noncoding RNA LINC01296 Harbors miR-21a to Regulate Colon Carcinoma Proliferation and Invasion |
title_short | Long Noncoding RNA LINC01296 Harbors miR-21a to Regulate Colon Carcinoma Proliferation and Invasion |
title_sort | long noncoding rna linc01296 harbors mir-21a to regulate colon carcinoma proliferation and invasion |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7848363/ https://www.ncbi.nlm.nih.gov/pubmed/29673421 http://dx.doi.org/10.3727/096504018X15234931503876 |
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