Cargando…

miR-630 Inhibits Epithelial-to-Mesenchymal Transition (EMT) by Regulating the Wnt/β-Catenin Pathway in Gastric Cancer Cells

Despite availability of different treatments, gastric cancer remains the second highest cause of cancer-related deaths worldwide. This study was aimed to explore the role of miR-630 in gastric cancer by investigating the underlying mechanism of inhibiting epithelial-to-mesenchymal transition (EMT) o...

Descripción completa

Detalles Bibliográficos
Autores principales: Li, Dong, Tian, Bo, Jin, Xiaosheng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cognizant Communication Corporation 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7848419/
https://www.ncbi.nlm.nih.gov/pubmed/29422112
http://dx.doi.org/10.3727/096504018X15178732625479
_version_ 1783645132408487936
author Li, Dong
Tian, Bo
Jin, Xiaosheng
author_facet Li, Dong
Tian, Bo
Jin, Xiaosheng
author_sort Li, Dong
collection PubMed
description Despite availability of different treatments, gastric cancer remains the second highest cause of cancer-related deaths worldwide. This study was aimed to explore the role of miR-630 in gastric cancer by investigating the underlying mechanism of inhibiting epithelial-to-mesenchymal transition (EMT) of SGC-7901 and BGC-823 cells through the Wnt/β-catenin pathway. Results showed that miR-630 was downregulated in several gastric cancer cell lines, including SGC-7901 and BGC-823. Transfection of miR-630 mimic showed a significant decrease in wound healing in a scratch assay in both cell lines compared to a scramble group. Also, transfection of miR-630 mimic inhibited cell viability, migration, and invasion of gastric cancer cells. miR-630 mimic transfection suppressed EMT by activating the Wnt/β-catenin pathway. This was supported by the fact that miR-630-mediated suppression of the EMT phenotype was reversed in the presence of the Wnt/β-catenin inhibitor ICG001. miR-630 plays a protective role against gastric cancer by suppressing EMT through activating the Wnt/β-catenin pathway.
format Online
Article
Text
id pubmed-7848419
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher Cognizant Communication Corporation
record_format MEDLINE/PubMed
spelling pubmed-78484192021-02-16 miR-630 Inhibits Epithelial-to-Mesenchymal Transition (EMT) by Regulating the Wnt/β-Catenin Pathway in Gastric Cancer Cells Li, Dong Tian, Bo Jin, Xiaosheng Oncol Res Article Despite availability of different treatments, gastric cancer remains the second highest cause of cancer-related deaths worldwide. This study was aimed to explore the role of miR-630 in gastric cancer by investigating the underlying mechanism of inhibiting epithelial-to-mesenchymal transition (EMT) of SGC-7901 and BGC-823 cells through the Wnt/β-catenin pathway. Results showed that miR-630 was downregulated in several gastric cancer cell lines, including SGC-7901 and BGC-823. Transfection of miR-630 mimic showed a significant decrease in wound healing in a scratch assay in both cell lines compared to a scramble group. Also, transfection of miR-630 mimic inhibited cell viability, migration, and invasion of gastric cancer cells. miR-630 mimic transfection suppressed EMT by activating the Wnt/β-catenin pathway. This was supported by the fact that miR-630-mediated suppression of the EMT phenotype was reversed in the presence of the Wnt/β-catenin inhibitor ICG001. miR-630 plays a protective role against gastric cancer by suppressing EMT through activating the Wnt/β-catenin pathway. Cognizant Communication Corporation 2018-12-27 /pmc/articles/PMC7848419/ /pubmed/29422112 http://dx.doi.org/10.3727/096504018X15178732625479 Text en Copyright © 2019 Cognizant, LLC. http://creativecommons.org/licenses/by-nc-nd/4.0/ This article is licensed under a Creative Commons Attribution-NonCommercial NoDerivatives 4.0 International License.
spellingShingle Article
Li, Dong
Tian, Bo
Jin, Xiaosheng
miR-630 Inhibits Epithelial-to-Mesenchymal Transition (EMT) by Regulating the Wnt/β-Catenin Pathway in Gastric Cancer Cells
title miR-630 Inhibits Epithelial-to-Mesenchymal Transition (EMT) by Regulating the Wnt/β-Catenin Pathway in Gastric Cancer Cells
title_full miR-630 Inhibits Epithelial-to-Mesenchymal Transition (EMT) by Regulating the Wnt/β-Catenin Pathway in Gastric Cancer Cells
title_fullStr miR-630 Inhibits Epithelial-to-Mesenchymal Transition (EMT) by Regulating the Wnt/β-Catenin Pathway in Gastric Cancer Cells
title_full_unstemmed miR-630 Inhibits Epithelial-to-Mesenchymal Transition (EMT) by Regulating the Wnt/β-Catenin Pathway in Gastric Cancer Cells
title_short miR-630 Inhibits Epithelial-to-Mesenchymal Transition (EMT) by Regulating the Wnt/β-Catenin Pathway in Gastric Cancer Cells
title_sort mir-630 inhibits epithelial-to-mesenchymal transition (emt) by regulating the wnt/β-catenin pathway in gastric cancer cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7848419/
https://www.ncbi.nlm.nih.gov/pubmed/29422112
http://dx.doi.org/10.3727/096504018X15178732625479
work_keys_str_mv AT lidong mir630inhibitsepithelialtomesenchymaltransitionemtbyregulatingthewntbcateninpathwayingastriccancercells
AT tianbo mir630inhibitsepithelialtomesenchymaltransitionemtbyregulatingthewntbcateninpathwayingastriccancercells
AT jinxiaosheng mir630inhibitsepithelialtomesenchymaltransitionemtbyregulatingthewntbcateninpathwayingastriccancercells