Cargando…

miR-203 Inhibits the Invasion and EMT of Gastric Cancer Cells by Directly Targeting Annexin A4

Many studies have shown that downregulated miR-203 level is in a variety of cancers including gastric cancer (GC). However, the precise molecule mechanisms of miR-203 in GC have not been well clarified. In the current study, we investigated the biological functions and molecular mechanisms of miR-20...

Descripción completa

Detalles Bibliográficos
Autores principales: Li, Jianye, Zhang, Bin, Cui, Jizhao, Liang, Zhen, Liu, Kexia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cognizant Communication Corporation 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7848421/
https://www.ncbi.nlm.nih.gov/pubmed/30837034
http://dx.doi.org/10.3727/096504018X15444387696532
_version_ 1783645132873007104
author Li, Jianye
Zhang, Bin
Cui, Jizhao
Liang, Zhen
Liu, Kexia
author_facet Li, Jianye
Zhang, Bin
Cui, Jizhao
Liang, Zhen
Liu, Kexia
author_sort Li, Jianye
collection PubMed
description Many studies have shown that downregulated miR-203 level is in a variety of cancers including gastric cancer (GC). However, the precise molecule mechanisms of miR-203 in GC have not been well clarified. In the current study, we investigated the biological functions and molecular mechanisms of miR-203 in GC cell lines. We found that miR-203 is downregulated in GC tissues and cell lines. Moreover, the low level of miR-203 was associated with increased expression of annexin A4 in GC tissues and cell lines. The invasion and EMT of GC cells were suppressed by overexpression of miR-203. However, downregulation of miR-203 promoted invasion and EMT of GC cells. Bioinformatics analysis predicted that annexin A4 was a potential target gene of miR-203. Next, luciferase reporter assay confirmed that miR-203 could directly target annexin A4. Consistent with the effect of miR-203, downregulation of annexin A4 by siRNA inhibited the invasion and EMT of GC cells. Introduction of annexin A4 in GC cells partially blocked the effects of miR-203 mimic. Introduction of miR-203 directly targeted annexin A4 to inhibit the invasion and EMT of GC cells. Overall, reactivation of the miR-203/annexin A4 axis may represent a new strategy for overcoming metastasis of GC.
format Online
Article
Text
id pubmed-7848421
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher Cognizant Communication Corporation
record_format MEDLINE/PubMed
spelling pubmed-78484212021-02-16 miR-203 Inhibits the Invasion and EMT of Gastric Cancer Cells by Directly Targeting Annexin A4 Li, Jianye Zhang, Bin Cui, Jizhao Liang, Zhen Liu, Kexia Oncol Res Article Many studies have shown that downregulated miR-203 level is in a variety of cancers including gastric cancer (GC). However, the precise molecule mechanisms of miR-203 in GC have not been well clarified. In the current study, we investigated the biological functions and molecular mechanisms of miR-203 in GC cell lines. We found that miR-203 is downregulated in GC tissues and cell lines. Moreover, the low level of miR-203 was associated with increased expression of annexin A4 in GC tissues and cell lines. The invasion and EMT of GC cells were suppressed by overexpression of miR-203. However, downregulation of miR-203 promoted invasion and EMT of GC cells. Bioinformatics analysis predicted that annexin A4 was a potential target gene of miR-203. Next, luciferase reporter assay confirmed that miR-203 could directly target annexin A4. Consistent with the effect of miR-203, downregulation of annexin A4 by siRNA inhibited the invasion and EMT of GC cells. Introduction of annexin A4 in GC cells partially blocked the effects of miR-203 mimic. Introduction of miR-203 directly targeted annexin A4 to inhibit the invasion and EMT of GC cells. Overall, reactivation of the miR-203/annexin A4 axis may represent a new strategy for overcoming metastasis of GC. Cognizant Communication Corporation 2019-07-12 /pmc/articles/PMC7848421/ /pubmed/30837034 http://dx.doi.org/10.3727/096504018X15444387696532 Text en Copyright © 2019 Cognizant, LLC. http://creativecommons.org/licenses/by-nc-nd/4.0/ This article is licensed under a Creative Commons Attribution-NonCommercial NoDerivatives 4.0 International License.
spellingShingle Article
Li, Jianye
Zhang, Bin
Cui, Jizhao
Liang, Zhen
Liu, Kexia
miR-203 Inhibits the Invasion and EMT of Gastric Cancer Cells by Directly Targeting Annexin A4
title miR-203 Inhibits the Invasion and EMT of Gastric Cancer Cells by Directly Targeting Annexin A4
title_full miR-203 Inhibits the Invasion and EMT of Gastric Cancer Cells by Directly Targeting Annexin A4
title_fullStr miR-203 Inhibits the Invasion and EMT of Gastric Cancer Cells by Directly Targeting Annexin A4
title_full_unstemmed miR-203 Inhibits the Invasion and EMT of Gastric Cancer Cells by Directly Targeting Annexin A4
title_short miR-203 Inhibits the Invasion and EMT of Gastric Cancer Cells by Directly Targeting Annexin A4
title_sort mir-203 inhibits the invasion and emt of gastric cancer cells by directly targeting annexin a4
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7848421/
https://www.ncbi.nlm.nih.gov/pubmed/30837034
http://dx.doi.org/10.3727/096504018X15444387696532
work_keys_str_mv AT lijianye mir203inhibitstheinvasionandemtofgastriccancercellsbydirectlytargetingannexina4
AT zhangbin mir203inhibitstheinvasionandemtofgastriccancercellsbydirectlytargetingannexina4
AT cuijizhao mir203inhibitstheinvasionandemtofgastriccancercellsbydirectlytargetingannexina4
AT liangzhen mir203inhibitstheinvasionandemtofgastriccancercellsbydirectlytargetingannexina4
AT liukexia mir203inhibitstheinvasionandemtofgastriccancercellsbydirectlytargetingannexina4