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miR-1284 Inhibits the Growth and Invasion of Breast Cancer Cells by Targeting ZIC2

miR-1284 has been reported to inhibit tumor growth in some human cancers, including lung cancer, ovarian cancer, and gastric cancer. Whether it regulates breast cancer progression remains elusive. In this study, we found that miR-1284 was downregulated in breast cancer tissues and cell lines compare...

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Detalles Bibliográficos
Autores principales: Zhang, Pengcheng, Yang, Fang, Luo, Qin, Yan, Daxue, Sun, Shengrong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cognizant Communication Corporation 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7848447/
https://www.ncbi.nlm.nih.gov/pubmed/30075825
http://dx.doi.org/10.3727/096504018X15242763477504
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author Zhang, Pengcheng
Yang, Fang
Luo, Qin
Yan, Daxue
Sun, Shengrong
author_facet Zhang, Pengcheng
Yang, Fang
Luo, Qin
Yan, Daxue
Sun, Shengrong
author_sort Zhang, Pengcheng
collection PubMed
description miR-1284 has been reported to inhibit tumor growth in some human cancers, including lung cancer, ovarian cancer, and gastric cancer. Whether it regulates breast cancer progression remains elusive. In this study, we found that miR-1284 was downregulated in breast cancer tissues and cell lines compared to normal control cells. Moreover, we showed that overexpression of miR-1284 significantly inhibited the proliferation, migration, and invasion of breast cancer cells while promoting apoptosis. In terms of mechanism, we found that transcription factor ZIC2 was a target of miR-1284 in breast cancer cells. Through the luciferase reporter assay, we demonstrated their direct interaction. RT-qPCR and Western blot also indicated that miR-1284 overexpression inhibited the protein levels of ZIC2 in breast cancer cells. Moreover, we found that ZIC2 knockdown inhibited the proliferation, migration, and invasion of breast cancer cells, whereas restoration of ZIC2 reversed the effects of miR-1284 on breast cancer cells. Taken together, our findings demonstrated that miR-1284 suppressed the proliferation, migration, and invasion of breast cancer cells via targeting ZIC2, which provided a new insight on the development of therapeutic targets for breast cancer treatment.
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spelling pubmed-78484472021-02-16 miR-1284 Inhibits the Growth and Invasion of Breast Cancer Cells by Targeting ZIC2 Zhang, Pengcheng Yang, Fang Luo, Qin Yan, Daxue Sun, Shengrong Oncol Res Article miR-1284 has been reported to inhibit tumor growth in some human cancers, including lung cancer, ovarian cancer, and gastric cancer. Whether it regulates breast cancer progression remains elusive. In this study, we found that miR-1284 was downregulated in breast cancer tissues and cell lines compared to normal control cells. Moreover, we showed that overexpression of miR-1284 significantly inhibited the proliferation, migration, and invasion of breast cancer cells while promoting apoptosis. In terms of mechanism, we found that transcription factor ZIC2 was a target of miR-1284 in breast cancer cells. Through the luciferase reporter assay, we demonstrated their direct interaction. RT-qPCR and Western blot also indicated that miR-1284 overexpression inhibited the protein levels of ZIC2 in breast cancer cells. Moreover, we found that ZIC2 knockdown inhibited the proliferation, migration, and invasion of breast cancer cells, whereas restoration of ZIC2 reversed the effects of miR-1284 on breast cancer cells. Taken together, our findings demonstrated that miR-1284 suppressed the proliferation, migration, and invasion of breast cancer cells via targeting ZIC2, which provided a new insight on the development of therapeutic targets for breast cancer treatment. Cognizant Communication Corporation 2019-02-05 /pmc/articles/PMC7848447/ /pubmed/30075825 http://dx.doi.org/10.3727/096504018X15242763477504 Text en Copyright © 2019 Cognizant, LLC. http://creativecommons.org/licenses/by-nc-nd/4.0/ This article is licensed under a Creative Commons Attribution-NonCommercial NoDerivatives 4.0 International License.
spellingShingle Article
Zhang, Pengcheng
Yang, Fang
Luo, Qin
Yan, Daxue
Sun, Shengrong
miR-1284 Inhibits the Growth and Invasion of Breast Cancer Cells by Targeting ZIC2
title miR-1284 Inhibits the Growth and Invasion of Breast Cancer Cells by Targeting ZIC2
title_full miR-1284 Inhibits the Growth and Invasion of Breast Cancer Cells by Targeting ZIC2
title_fullStr miR-1284 Inhibits the Growth and Invasion of Breast Cancer Cells by Targeting ZIC2
title_full_unstemmed miR-1284 Inhibits the Growth and Invasion of Breast Cancer Cells by Targeting ZIC2
title_short miR-1284 Inhibits the Growth and Invasion of Breast Cancer Cells by Targeting ZIC2
title_sort mir-1284 inhibits the growth and invasion of breast cancer cells by targeting zic2
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7848447/
https://www.ncbi.nlm.nih.gov/pubmed/30075825
http://dx.doi.org/10.3727/096504018X15242763477504
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