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CD47 Promotes Human Glioblastoma Invasion Through Activation of the PI3K/Akt Pathway

Cluster of differentiation 47 (CD47) overexpression is common in various malignancies. This study investigated whether CD47 promotes human glioblastoma invasion and, if so, the underlying mechanisms involved. CD47 expression was found to be stronger in tissues of patients with glioblastoma and in va...

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Detalles Bibliográficos
Autores principales: Liu, Xuejian, Wu, Xia, Wang, Yanming, Li, Yuhua, Chen, Xiangli, Yang, Wenchuan, Jiang, Lihua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cognizant Communication Corporation 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7848455/
https://www.ncbi.nlm.nih.gov/pubmed/29321087
http://dx.doi.org/10.3727/096504018X15155538502359
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author Liu, Xuejian
Wu, Xia
Wang, Yanming
Li, Yuhua
Chen, Xiangli
Yang, Wenchuan
Jiang, Lihua
author_facet Liu, Xuejian
Wu, Xia
Wang, Yanming
Li, Yuhua
Chen, Xiangli
Yang, Wenchuan
Jiang, Lihua
author_sort Liu, Xuejian
collection PubMed
description Cluster of differentiation 47 (CD47) overexpression is common in various malignancies. This study investigated whether CD47 promotes human glioblastoma invasion and, if so, the underlying mechanisms involved. CD47 expression was found to be stronger in tissues of patients with glioblastoma and in various cancer cell lines than in normal controls. CD47 downregulation via siRNA suppressed invasion in vitro, whereas CD47 overexpression through plasmid transfection exerted the opposite effect. However, overexpression or knocking down of CD47 had no effect on cell proliferation. Moreover, CD47 expression was related to Akt phosphorylation at the cellular molecular level. Suppression of Akt with a specific inhibitor impaired the invasion ability of CD47-overexpressing cells, indicating that stimulation of the PI3K/Akt pathway served as the downstream regulator of CD47-triggered invasion. These results suggest that CD47 might be a useful predictor of poor prognosis and metastasis and a potential target for treating glioblastomas.
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spelling pubmed-78484552021-02-16 CD47 Promotes Human Glioblastoma Invasion Through Activation of the PI3K/Akt Pathway Liu, Xuejian Wu, Xia Wang, Yanming Li, Yuhua Chen, Xiangli Yang, Wenchuan Jiang, Lihua Oncol Res Article Cluster of differentiation 47 (CD47) overexpression is common in various malignancies. This study investigated whether CD47 promotes human glioblastoma invasion and, if so, the underlying mechanisms involved. CD47 expression was found to be stronger in tissues of patients with glioblastoma and in various cancer cell lines than in normal controls. CD47 downregulation via siRNA suppressed invasion in vitro, whereas CD47 overexpression through plasmid transfection exerted the opposite effect. However, overexpression or knocking down of CD47 had no effect on cell proliferation. Moreover, CD47 expression was related to Akt phosphorylation at the cellular molecular level. Suppression of Akt with a specific inhibitor impaired the invasion ability of CD47-overexpressing cells, indicating that stimulation of the PI3K/Akt pathway served as the downstream regulator of CD47-triggered invasion. These results suggest that CD47 might be a useful predictor of poor prognosis and metastasis and a potential target for treating glioblastomas. Cognizant Communication Corporation 2019-03-29 /pmc/articles/PMC7848455/ /pubmed/29321087 http://dx.doi.org/10.3727/096504018X15155538502359 Text en Copyright © 2019 Cognizant, LLC. http://creativecommons.org/licenses/by-nc-nd/4.0/ This article is licensed under a Creative Commons Attribution-NonCommercial NoDerivatives 4.0 International License.
spellingShingle Article
Liu, Xuejian
Wu, Xia
Wang, Yanming
Li, Yuhua
Chen, Xiangli
Yang, Wenchuan
Jiang, Lihua
CD47 Promotes Human Glioblastoma Invasion Through Activation of the PI3K/Akt Pathway
title CD47 Promotes Human Glioblastoma Invasion Through Activation of the PI3K/Akt Pathway
title_full CD47 Promotes Human Glioblastoma Invasion Through Activation of the PI3K/Akt Pathway
title_fullStr CD47 Promotes Human Glioblastoma Invasion Through Activation of the PI3K/Akt Pathway
title_full_unstemmed CD47 Promotes Human Glioblastoma Invasion Through Activation of the PI3K/Akt Pathway
title_short CD47 Promotes Human Glioblastoma Invasion Through Activation of the PI3K/Akt Pathway
title_sort cd47 promotes human glioblastoma invasion through activation of the pi3k/akt pathway
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7848455/
https://www.ncbi.nlm.nih.gov/pubmed/29321087
http://dx.doi.org/10.3727/096504018X15155538502359
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