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Impact of chemical mutagenesis using ethyl methane sulphonate on tepary bean seedling vigour and adult plant performance
Tepary bean is an important food legume. The genetic improvement of the crop is limited by a narrow genetic base but this genetic base can be broadened through induced mutagenesis. This study was designed to determine the (i) effects of chemical mutagenesis using ethyl methane sulfonate (EMS) on M(1...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7848653/ https://www.ncbi.nlm.nih.gov/pubmed/33553760 http://dx.doi.org/10.1016/j.heliyon.2021.e06103 |
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author | Thangwana, Andries Gwata, Eastonce T. Zhou, Marvelous M. |
author_facet | Thangwana, Andries Gwata, Eastonce T. Zhou, Marvelous M. |
author_sort | Thangwana, Andries |
collection | PubMed |
description | Tepary bean is an important food legume. The genetic improvement of the crop is limited by a narrow genetic base but this genetic base can be broadened through induced mutagenesis. This study was designed to determine the (i) effects of chemical mutagenesis using ethyl methane sulfonate (EMS) on M(1) seedlings (ii) the adult plant performance of M(2) - M(4) generations and (iii) the relationships between growth attributes at the seedling and adult plant stage. Mutagenized seed with varying doses of EMS was germinated at room temperature in order to raise M(1) seedlings. There were highly significant (P < 0.01) differences due to dose effects among the seedlings. The highest LD(50) (3.37 % EMS v/v) was observed for ‘Genotype 3’. Under field conditions, all the three factors influenced the plant performance. The results demonstrated the potential of EMS to induce genotypic variation and desirable agronomic traits in tepary bean. |
format | Online Article Text |
id | pubmed-7848653 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-78486532021-02-04 Impact of chemical mutagenesis using ethyl methane sulphonate on tepary bean seedling vigour and adult plant performance Thangwana, Andries Gwata, Eastonce T. Zhou, Marvelous M. Heliyon Research Article Tepary bean is an important food legume. The genetic improvement of the crop is limited by a narrow genetic base but this genetic base can be broadened through induced mutagenesis. This study was designed to determine the (i) effects of chemical mutagenesis using ethyl methane sulfonate (EMS) on M(1) seedlings (ii) the adult plant performance of M(2) - M(4) generations and (iii) the relationships between growth attributes at the seedling and adult plant stage. Mutagenized seed with varying doses of EMS was germinated at room temperature in order to raise M(1) seedlings. There were highly significant (P < 0.01) differences due to dose effects among the seedlings. The highest LD(50) (3.37 % EMS v/v) was observed for ‘Genotype 3’. Under field conditions, all the three factors influenced the plant performance. The results demonstrated the potential of EMS to induce genotypic variation and desirable agronomic traits in tepary bean. Elsevier 2021-01-29 /pmc/articles/PMC7848653/ /pubmed/33553760 http://dx.doi.org/10.1016/j.heliyon.2021.e06103 Text en © 2021 The Author(s) http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Research Article Thangwana, Andries Gwata, Eastonce T. Zhou, Marvelous M. Impact of chemical mutagenesis using ethyl methane sulphonate on tepary bean seedling vigour and adult plant performance |
title | Impact of chemical mutagenesis using ethyl methane sulphonate on tepary bean seedling vigour and adult plant performance |
title_full | Impact of chemical mutagenesis using ethyl methane sulphonate on tepary bean seedling vigour and adult plant performance |
title_fullStr | Impact of chemical mutagenesis using ethyl methane sulphonate on tepary bean seedling vigour and adult plant performance |
title_full_unstemmed | Impact of chemical mutagenesis using ethyl methane sulphonate on tepary bean seedling vigour and adult plant performance |
title_short | Impact of chemical mutagenesis using ethyl methane sulphonate on tepary bean seedling vigour and adult plant performance |
title_sort | impact of chemical mutagenesis using ethyl methane sulphonate on tepary bean seedling vigour and adult plant performance |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7848653/ https://www.ncbi.nlm.nih.gov/pubmed/33553760 http://dx.doi.org/10.1016/j.heliyon.2021.e06103 |
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