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Two-step strategy for the identification of SARS-CoV-2 variant of concern 202012/01 and other variants with spike deletion H69–V70, France, August to December 2020
We report the strategy leading to the first detection of variant of concern 202012/01 (VOC) in France (21 December 2020). First, the spike (S) deletion H69–V70 (ΔH69/ΔV70), identified in certain SARS-CoV-2 variants including VOC, is screened for. This deletion is associated with a S-gene target fail...
| Autores principales: | , , , , , , , , , , , , , , , , , , , , , , , , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
European Centre for Disease Prevention and Control (ECDC)
2021
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7848679/ https://www.ncbi.nlm.nih.gov/pubmed/33478625 http://dx.doi.org/10.2807/1560-7917.ES.2021.26.3.2100008 |
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| author | Bal, Antonin Destras, Gregory Gaymard, Alexandre Stefic, Karl Marlet, Julien Eymieux, Sébastien Regue, Hadrien Semanas, Quentin d’Aubarede, Constance Billaud, Geneviève Laurent, Frédéric Gonzalez, Claudia Mekki, Yahia Valette, Martine Bouscambert, Maude Gaudy-Graffin, Catherine Lina, Bruno Morfin, Florence Josset, Laurence Casalegno, Jean-Sébastien Frobert, Emilie Escuret, Vanessa Icard, Vinca Jeannoel, Marion Milon, Marie-Paule Ramière, Christophe Scholtès, Caroline Tardy, Jean-Claude Trabaud, Mary-Anne Schuffenecker, Isabelle |
| author_facet | Bal, Antonin Destras, Gregory Gaymard, Alexandre Stefic, Karl Marlet, Julien Eymieux, Sébastien Regue, Hadrien Semanas, Quentin d’Aubarede, Constance Billaud, Geneviève Laurent, Frédéric Gonzalez, Claudia Mekki, Yahia Valette, Martine Bouscambert, Maude Gaudy-Graffin, Catherine Lina, Bruno Morfin, Florence Josset, Laurence Casalegno, Jean-Sébastien Frobert, Emilie Escuret, Vanessa Icard, Vinca Jeannoel, Marion Milon, Marie-Paule Ramière, Christophe Scholtès, Caroline Tardy, Jean-Claude Trabaud, Mary-Anne Schuffenecker, Isabelle |
| author_sort | Bal, Antonin |
| collection | PubMed |
| description | We report the strategy leading to the first detection of variant of concern 202012/01 (VOC) in France (21 December 2020). First, the spike (S) deletion H69–V70 (ΔH69/ΔV70), identified in certain SARS-CoV-2 variants including VOC, is screened for. This deletion is associated with a S-gene target failure (SGTF) in the three-target RT-PCR assay (TaqPath kit). Subsequently, SGTF samples are whole genome sequenced. This approach revealed mutations co-occurring with ΔH69/ΔV70 including S:N501Y in the VOC. |
| format | Online Article Text |
| id | pubmed-7848679 |
| institution | National Center for Biotechnology Information |
| language | English |
| publishDate | 2021 |
| publisher | European Centre for Disease Prevention and Control (ECDC) |
| record_format | MEDLINE/PubMed |
| spelling | pubmed-78486792021-02-05 Two-step strategy for the identification of SARS-CoV-2 variant of concern 202012/01 and other variants with spike deletion H69–V70, France, August to December 2020 Bal, Antonin Destras, Gregory Gaymard, Alexandre Stefic, Karl Marlet, Julien Eymieux, Sébastien Regue, Hadrien Semanas, Quentin d’Aubarede, Constance Billaud, Geneviève Laurent, Frédéric Gonzalez, Claudia Mekki, Yahia Valette, Martine Bouscambert, Maude Gaudy-Graffin, Catherine Lina, Bruno Morfin, Florence Josset, Laurence Casalegno, Jean-Sébastien Frobert, Emilie Escuret, Vanessa Icard, Vinca Jeannoel, Marion Milon, Marie-Paule Ramière, Christophe Scholtès, Caroline Tardy, Jean-Claude Trabaud, Mary-Anne Schuffenecker, Isabelle Euro Surveill Rapid Communication We report the strategy leading to the first detection of variant of concern 202012/01 (VOC) in France (21 December 2020). First, the spike (S) deletion H69–V70 (ΔH69/ΔV70), identified in certain SARS-CoV-2 variants including VOC, is screened for. This deletion is associated with a S-gene target failure (SGTF) in the three-target RT-PCR assay (TaqPath kit). Subsequently, SGTF samples are whole genome sequenced. This approach revealed mutations co-occurring with ΔH69/ΔV70 including S:N501Y in the VOC. European Centre for Disease Prevention and Control (ECDC) 2021-01-21 /pmc/articles/PMC7848679/ /pubmed/33478625 http://dx.doi.org/10.2807/1560-7917.ES.2021.26.3.2100008 Text en This article is copyright of the authors or their affiliated institutions, 2021. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution (CC BY 4.0) Licence. You may share and adapt the material, but must give appropriate credit to the source, provide a link to the licence, and indicate if changes were made. |
| spellingShingle | Rapid Communication Bal, Antonin Destras, Gregory Gaymard, Alexandre Stefic, Karl Marlet, Julien Eymieux, Sébastien Regue, Hadrien Semanas, Quentin d’Aubarede, Constance Billaud, Geneviève Laurent, Frédéric Gonzalez, Claudia Mekki, Yahia Valette, Martine Bouscambert, Maude Gaudy-Graffin, Catherine Lina, Bruno Morfin, Florence Josset, Laurence Casalegno, Jean-Sébastien Frobert, Emilie Escuret, Vanessa Icard, Vinca Jeannoel, Marion Milon, Marie-Paule Ramière, Christophe Scholtès, Caroline Tardy, Jean-Claude Trabaud, Mary-Anne Schuffenecker, Isabelle Two-step strategy for the identification of SARS-CoV-2 variant of concern 202012/01 and other variants with spike deletion H69–V70, France, August to December 2020 |
| title | Two-step strategy for the identification of SARS-CoV-2 variant of concern 202012/01 and other variants with spike deletion H69–V70, France, August to December 2020 |
| title_full | Two-step strategy for the identification of SARS-CoV-2 variant of concern 202012/01 and other variants with spike deletion H69–V70, France, August to December 2020 |
| title_fullStr | Two-step strategy for the identification of SARS-CoV-2 variant of concern 202012/01 and other variants with spike deletion H69–V70, France, August to December 2020 |
| title_full_unstemmed | Two-step strategy for the identification of SARS-CoV-2 variant of concern 202012/01 and other variants with spike deletion H69–V70, France, August to December 2020 |
| title_short | Two-step strategy for the identification of SARS-CoV-2 variant of concern 202012/01 and other variants with spike deletion H69–V70, France, August to December 2020 |
| title_sort | two-step strategy for the identification of sars-cov-2 variant of concern 202012/01 and other variants with spike deletion h69–v70, france, august to december 2020 |
| topic | Rapid Communication |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7848679/ https://www.ncbi.nlm.nih.gov/pubmed/33478625 http://dx.doi.org/10.2807/1560-7917.ES.2021.26.3.2100008 |
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