Regional N-glycan and lipid analysis from tissues using MALDI-mass spectrometry imaging

N-glycans and lipids are structural metabolites that play important roles in cellular processes. Both show unique regional distribution in tissues; therefore, spatial analyses of these metabolites are crucial to our understanding of cellular physiology. Matrix-assisted laser desorption/ionization-ma...

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Detalles Bibliográficos
Autores principales: Stanback, Alexandra E., Conroy, Lindsey R., Young, Lyndsay E.A., Hawkinson, Tara R., Markussen, Kia H., Clarke, Harrison A., Allison, Derek B., Sun, Ramon C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7848795/
https://www.ncbi.nlm.nih.gov/pubmed/33554139
http://dx.doi.org/10.1016/j.xpro.2021.100304
Descripción
Sumario:N-glycans and lipids are structural metabolites that play important roles in cellular processes. Both show unique regional distribution in tissues; therefore, spatial analyses of these metabolites are crucial to our understanding of cellular physiology. Matrix-assisted laser desorption/ionization-mass spectrometry imaging (MALDI-MSI) is an innovative technique that enables in situ detection of analytes with spatial distribution. This workflow details a MALDI-MSI protocol for the spatial profiling of N-glycans and lipids from tissues following application of enzyme and MALDI matrix. For complete details on the use and execution of this protocol, please refer to Drake et al. (2018) and Andres et al. (2020).

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