Regional N-glycan and lipid analysis from tissues using MALDI-mass spectrometry imaging

N-glycans and lipids are structural metabolites that play important roles in cellular processes. Both show unique regional distribution in tissues; therefore, spatial analyses of these metabolites are crucial to our understanding of cellular physiology. Matrix-assisted laser desorption/ionization-ma...

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Autores principales: Stanback, Alexandra E., Conroy, Lindsey R., Young, Lyndsay E.A., Hawkinson, Tara R., Markussen, Kia H., Clarke, Harrison A., Allison, Derek B., Sun, Ramon C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7848795/
https://www.ncbi.nlm.nih.gov/pubmed/33554139
http://dx.doi.org/10.1016/j.xpro.2021.100304
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author Stanback, Alexandra E.
Conroy, Lindsey R.
Young, Lyndsay E.A.
Hawkinson, Tara R.
Markussen, Kia H.
Clarke, Harrison A.
Allison, Derek B.
Sun, Ramon C.
author_facet Stanback, Alexandra E.
Conroy, Lindsey R.
Young, Lyndsay E.A.
Hawkinson, Tara R.
Markussen, Kia H.
Clarke, Harrison A.
Allison, Derek B.
Sun, Ramon C.
author_sort Stanback, Alexandra E.
collection PubMed
description N-glycans and lipids are structural metabolites that play important roles in cellular processes. Both show unique regional distribution in tissues; therefore, spatial analyses of these metabolites are crucial to our understanding of cellular physiology. Matrix-assisted laser desorption/ionization-mass spectrometry imaging (MALDI-MSI) is an innovative technique that enables in situ detection of analytes with spatial distribution. This workflow details a MALDI-MSI protocol for the spatial profiling of N-glycans and lipids from tissues following application of enzyme and MALDI matrix. For complete details on the use and execution of this protocol, please refer to Drake et al. (2018) and Andres et al. (2020).
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spelling pubmed-78487952021-02-05 Regional N-glycan and lipid analysis from tissues using MALDI-mass spectrometry imaging Stanback, Alexandra E. Conroy, Lindsey R. Young, Lyndsay E.A. Hawkinson, Tara R. Markussen, Kia H. Clarke, Harrison A. Allison, Derek B. Sun, Ramon C. STAR Protoc Protocol N-glycans and lipids are structural metabolites that play important roles in cellular processes. Both show unique regional distribution in tissues; therefore, spatial analyses of these metabolites are crucial to our understanding of cellular physiology. Matrix-assisted laser desorption/ionization-mass spectrometry imaging (MALDI-MSI) is an innovative technique that enables in situ detection of analytes with spatial distribution. This workflow details a MALDI-MSI protocol for the spatial profiling of N-glycans and lipids from tissues following application of enzyme and MALDI matrix. For complete details on the use and execution of this protocol, please refer to Drake et al. (2018) and Andres et al. (2020). Elsevier 2021-01-29 /pmc/articles/PMC7848795/ /pubmed/33554139 http://dx.doi.org/10.1016/j.xpro.2021.100304 Text en © 2021 The Author(s) http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Stanback, Alexandra E.
Conroy, Lindsey R.
Young, Lyndsay E.A.
Hawkinson, Tara R.
Markussen, Kia H.
Clarke, Harrison A.
Allison, Derek B.
Sun, Ramon C.
Regional N-glycan and lipid analysis from tissues using MALDI-mass spectrometry imaging
title Regional N-glycan and lipid analysis from tissues using MALDI-mass spectrometry imaging
title_full Regional N-glycan and lipid analysis from tissues using MALDI-mass spectrometry imaging
title_fullStr Regional N-glycan and lipid analysis from tissues using MALDI-mass spectrometry imaging
title_full_unstemmed Regional N-glycan and lipid analysis from tissues using MALDI-mass spectrometry imaging
title_short Regional N-glycan and lipid analysis from tissues using MALDI-mass spectrometry imaging
title_sort regional n-glycan and lipid analysis from tissues using maldi-mass spectrometry imaging
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7848795/
https://www.ncbi.nlm.nih.gov/pubmed/33554139
http://dx.doi.org/10.1016/j.xpro.2021.100304
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