Nondestructive enzymatic deamination enables single-molecule long-read amplicon sequencing for the determination of 5-methylcytosine and 5-hydroxymethylcytosine at single-base resolution

The predominant methodology for DNA methylation analysis relies on the chemical deamination by sodium bisulfite of unmodified cytosine to uracil to permit the differential readout of methylated cytosines. Bisulfite treatment damages the DNA, leading to fragmentation and loss of long-range methylatio...

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Detalles Bibliográficos
Autores principales: Sun, Zhiyi, Vaisvila, Romualdas, Hussong, Laura-Madison, Yan, Bo, Baum, Chloé, Saleh, Lana, Samaranayake, Mala, Guan, Shengxi, Dai, Nan, Corrêa, Ivan R., Pradhan, Sriharsa, Davis, Theodore B., Evans, Thomas C., Ettwiller, Laurence M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory Press 2021
Materias:
Method
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7849414/
https://www.ncbi.nlm.nih.gov/pubmed/33468551
http://dx.doi.org/10.1101/gr.265306.120

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7849414/
https://www.ncbi.nlm.nih.gov/pubmed/33468551
http://dx.doi.org/10.1101/gr.265306.120

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