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Genotyping tools and resources to assess peanut germplasm: smut-resistant landraces as a case study

Peanut smut caused by Thecaphora frezii is a severe fungal disease currently endemic to Argentina and Brazil. The identification of smut resistant germplasm is crucial in view of the potential risk of a global spread. In a recent study, we reported new sources of smut resistance and demonstrated its...

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Autores principales: Massa, Alicia N., Bressano, Marina, Soave, Juan H., Buteler, Mario I., Seijo, Guillermo, Sobolev, Victor S., Orner, Valerie A., Oddino, Claudio, Soave, Sara J., Faustinelli, Paola C., de Blas, Francisco J., Lamb, Marshall C., Arias, Renee S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: PeerJ Inc. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7849506/
https://www.ncbi.nlm.nih.gov/pubmed/33575123
http://dx.doi.org/10.7717/peerj.10581
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author Massa, Alicia N.
Bressano, Marina
Soave, Juan H.
Buteler, Mario I.
Seijo, Guillermo
Sobolev, Victor S.
Orner, Valerie A.
Oddino, Claudio
Soave, Sara J.
Faustinelli, Paola C.
de Blas, Francisco J.
Lamb, Marshall C.
Arias, Renee S.
author_facet Massa, Alicia N.
Bressano, Marina
Soave, Juan H.
Buteler, Mario I.
Seijo, Guillermo
Sobolev, Victor S.
Orner, Valerie A.
Oddino, Claudio
Soave, Sara J.
Faustinelli, Paola C.
de Blas, Francisco J.
Lamb, Marshall C.
Arias, Renee S.
author_sort Massa, Alicia N.
collection PubMed
description Peanut smut caused by Thecaphora frezii is a severe fungal disease currently endemic to Argentina and Brazil. The identification of smut resistant germplasm is crucial in view of the potential risk of a global spread. In a recent study, we reported new sources of smut resistance and demonstrated its introgression into elite peanut cultivars. Here, we revisited one of these sources (line I0322) to verify its presence in the U.S. peanut germplasm collection and to identify single nucleotide polymorphisms (SNPs) potentially associated with resistance. Five accessions of Arachis hypogaea subsp. fastigiata from the U.S. peanut collection, along with the resistant source and derived inbred lines were genotyped with a 48K SNP peanut array. A recently developed SNP genotyping platform called RNase H2 enzyme-based amplification (rhAmp) was further applied to validate selected SNPs in a larger number of individuals per accession. More than 14,000 SNPs and nine rhAmp assays confirmed the presence of a germplasm in the U.S. peanut collection that is 98.6% identical (P < 0.01, bootstrap t-test) to the resistant line I0322. We report this germplasm with accompanying genetic information, genotyping data, and diagnostic SNP markers.
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spelling pubmed-78495062021-02-10 Genotyping tools and resources to assess peanut germplasm: smut-resistant landraces as a case study Massa, Alicia N. Bressano, Marina Soave, Juan H. Buteler, Mario I. Seijo, Guillermo Sobolev, Victor S. Orner, Valerie A. Oddino, Claudio Soave, Sara J. Faustinelli, Paola C. de Blas, Francisco J. Lamb, Marshall C. Arias, Renee S. PeerJ Agricultural Science Peanut smut caused by Thecaphora frezii is a severe fungal disease currently endemic to Argentina and Brazil. The identification of smut resistant germplasm is crucial in view of the potential risk of a global spread. In a recent study, we reported new sources of smut resistance and demonstrated its introgression into elite peanut cultivars. Here, we revisited one of these sources (line I0322) to verify its presence in the U.S. peanut germplasm collection and to identify single nucleotide polymorphisms (SNPs) potentially associated with resistance. Five accessions of Arachis hypogaea subsp. fastigiata from the U.S. peanut collection, along with the resistant source and derived inbred lines were genotyped with a 48K SNP peanut array. A recently developed SNP genotyping platform called RNase H2 enzyme-based amplification (rhAmp) was further applied to validate selected SNPs in a larger number of individuals per accession. More than 14,000 SNPs and nine rhAmp assays confirmed the presence of a germplasm in the U.S. peanut collection that is 98.6% identical (P < 0.01, bootstrap t-test) to the resistant line I0322. We report this germplasm with accompanying genetic information, genotyping data, and diagnostic SNP markers. PeerJ Inc. 2021-01-29 /pmc/articles/PMC7849506/ /pubmed/33575123 http://dx.doi.org/10.7717/peerj.10581 Text en https://creativecommons.org/publicdomain/zero/1.0/ This is an open access article, free of all copyright, made available under the Creative Commons Public Domain Dedication (https://creativecommons.org/publicdomain/zero/1.0/) . This work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.
spellingShingle Agricultural Science
Massa, Alicia N.
Bressano, Marina
Soave, Juan H.
Buteler, Mario I.
Seijo, Guillermo
Sobolev, Victor S.
Orner, Valerie A.
Oddino, Claudio
Soave, Sara J.
Faustinelli, Paola C.
de Blas, Francisco J.
Lamb, Marshall C.
Arias, Renee S.
Genotyping tools and resources to assess peanut germplasm: smut-resistant landraces as a case study
title Genotyping tools and resources to assess peanut germplasm: smut-resistant landraces as a case study
title_full Genotyping tools and resources to assess peanut germplasm: smut-resistant landraces as a case study
title_fullStr Genotyping tools and resources to assess peanut germplasm: smut-resistant landraces as a case study
title_full_unstemmed Genotyping tools and resources to assess peanut germplasm: smut-resistant landraces as a case study
title_short Genotyping tools and resources to assess peanut germplasm: smut-resistant landraces as a case study
title_sort genotyping tools and resources to assess peanut germplasm: smut-resistant landraces as a case study
topic Agricultural Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7849506/
https://www.ncbi.nlm.nih.gov/pubmed/33575123
http://dx.doi.org/10.7717/peerj.10581
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