Clinical validation of an automated reverse transcription-insulated isothermal PCR assay for the detection of severe acute respiratory syndrome coronavirus 2

To determine clinical performance of the single-target SARS-CoV-2 orf 1 ab reverse transcription-insulated isothermal PCR (RT-iiPCR) assay, the positive percentage agreement between this assay and a laboratory real-time RT-PCR assay was 96.8% (30 of 31; 95% confidence interval [CI], 90.5%–100%) and...

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Autores principales: Chang, Pai-Ling, Lin, Chia-Yi, Chen, Cheng-Pin, Lin, Yi-Chun, Hu, Hui-Chun, Cheng, Shu-Hsing, Cheng, Chien-Yu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taiwan Society of Microbiology. Published by Elsevier Taiwan LLC. 2021
Materias:
Short Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7849545/
https://www.ncbi.nlm.nih.gov/pubmed/33582073
http://dx.doi.org/10.1016/j.jmii.2021.01.005
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author Chang, Pai-Ling
Lin, Chia-Yi
Chen, Cheng-Pin
Lin, Yi-Chun
Hu, Hui-Chun
Cheng, Shu-Hsing
Cheng, Chien-Yu
author_facet Chang, Pai-Ling
Lin, Chia-Yi
Chen, Cheng-Pin
Lin, Yi-Chun
Hu, Hui-Chun
Cheng, Shu-Hsing
Cheng, Chien-Yu
author_sort Chang, Pai-Ling
collection PubMed
description To determine clinical performance of the single-target SARS-CoV-2 orf 1 ab reverse transcription-insulated isothermal PCR (RT-iiPCR) assay, the positive percentage agreement between this assay and a laboratory real-time RT-PCR assay was 96.8% (30 of 31; 95% confidence interval [CI], 90.5%–100%) and the negative percentage agreement was 97.1% (67 of 69; 95% CI, 93.1%–100%).
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spelling pubmed-78495452021-02-02 Clinical validation of an automated reverse transcription-insulated isothermal PCR assay for the detection of severe acute respiratory syndrome coronavirus 2 Chang, Pai-Ling Lin, Chia-Yi Chen, Cheng-Pin Lin, Yi-Chun Hu, Hui-Chun Cheng, Shu-Hsing Cheng, Chien-Yu J Microbiol Immunol Infect Short Communication To determine clinical performance of the single-target SARS-CoV-2 orf 1 ab reverse transcription-insulated isothermal PCR (RT-iiPCR) assay, the positive percentage agreement between this assay and a laboratory real-time RT-PCR assay was 96.8% (30 of 31; 95% confidence interval [CI], 90.5%–100%) and the negative percentage agreement was 97.1% (67 of 69; 95% CI, 93.1%–100%). Taiwan Society of Microbiology. Published by Elsevier Taiwan LLC. 2021-06 2021-02-01 /pmc/articles/PMC7849545/ /pubmed/33582073 http://dx.doi.org/10.1016/j.jmii.2021.01.005 Text en © 2021 Taiwan Society of Microbiology. Published by Elsevier Taiwan LLC. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Short Communication
Chang, Pai-Ling
Lin, Chia-Yi
Chen, Cheng-Pin
Lin, Yi-Chun
Hu, Hui-Chun
Cheng, Shu-Hsing
Cheng, Chien-Yu
Clinical validation of an automated reverse transcription-insulated isothermal PCR assay for the detection of severe acute respiratory syndrome coronavirus 2
title Clinical validation of an automated reverse transcription-insulated isothermal PCR assay for the detection of severe acute respiratory syndrome coronavirus 2
title_full Clinical validation of an automated reverse transcription-insulated isothermal PCR assay for the detection of severe acute respiratory syndrome coronavirus 2
title_fullStr Clinical validation of an automated reverse transcription-insulated isothermal PCR assay for the detection of severe acute respiratory syndrome coronavirus 2
title_full_unstemmed Clinical validation of an automated reverse transcription-insulated isothermal PCR assay for the detection of severe acute respiratory syndrome coronavirus 2
title_short Clinical validation of an automated reverse transcription-insulated isothermal PCR assay for the detection of severe acute respiratory syndrome coronavirus 2
title_sort clinical validation of an automated reverse transcription-insulated isothermal pcr assay for the detection of severe acute respiratory syndrome coronavirus 2
topic Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7849545/
https://www.ncbi.nlm.nih.gov/pubmed/33582073
http://dx.doi.org/10.1016/j.jmii.2021.01.005
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