Cargando…

Clinical validation of an automated reverse transcription-insulated isothermal PCR assay for the detection of severe acute respiratory syndrome coronavirus 2

To determine clinical performance of the single-target SARS-CoV-2 orf 1 ab reverse transcription-insulated isothermal PCR (RT-iiPCR) assay, the positive percentage agreement between this assay and a laboratory real-time RT-PCR assay was 96.8% (30 of 31; 95% confidence interval [CI], 90.5%–100%) and...

Descripción completa

Detalles Bibliográficos
Autores principales:	Chang, Pai-Ling, Lin, Chia-Yi, Chen, Cheng-Pin, Lin, Yi-Chun, Hu, Hui-Chun, Cheng, Shu-Hsing, Cheng, Chien-Yu
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Taiwan Society of Microbiology. Published by Elsevier Taiwan LLC. 2021
Materias:	Short Communication
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7849545/ https://www.ncbi.nlm.nih.gov/pubmed/33582073 http://dx.doi.org/10.1016/j.jmii.2021.01.005

Ejemplares similares

Evaluation and Clinical Validation of Two Field–Deployable Reverse Transcription-Insulated Isothermal PCR Assays for the Detection of the Middle East Respiratory Syndrome–Coronavirus
por: Go, Yun Young, et al.
Publicado: (2017)

Development and use of a reverse transcription insulated isothermal PCR assay for detection and characterization of bovine torovirus in yaks
por: Zhao, Long, et al.
Publicado: (2021)

Development and evaluation of reverse transcription-insulated isothermal PCR assay to detect duck hepatitis A virus type A in liver samples using the POCKIT(TM) system
por: REN, Yupeng, et al.
Publicado: (2019)

Evaluation of a field-deployable reverse transcription-insulated isothermal PCR for rapid and sensitive on-site detection of Zika virus
por: Carossino, Mariano, et al.
Publicado: (2017)

Detection of respiratory viruses in adults with respiratory tract infection using a multiplex PCR assay at a tertiary center
por: Tai, Cheng-Chun, et al.
Publicado: (2020)

Development of insulated isothermal PCR for rapid on-site malaria detection
por: Chua, Kek Heng, et al.
Publicado: (2016)

A Field-Deployable Insulated Isothermal PCR (iiPCR) for the Global Surveillance of Toxoplasma gondii Infection in Cetaceans
por: Hsieh, Meng-Jung, et al.
Publicado: (2022)

Evaluation of two singleplex reverse transcription-Insulated isothermal PCR tests and a duplex real-time RT-PCR test for the detection of porcine epidemic diarrhea virus and porcine deltacoronavirus
por: Zhang, Jianqiang, et al.
Publicado: (2016)

Rapid and sensitive insulated isothermal PCR for point-of-need feline leukaemia virus detection
por: Wilkes, Rebecca P, et al.
Publicado: (2018)

Development of TaqMan Probe-Based Insulated Isothermal PCR (iiPCR) for Sensitive and Specific On-Site Pathogen Detection
por: Tsai, Yun-Long, et al.
Publicado: (2012)

Rapid Detection of Fusarium oxysporum Using Insulated Isothermal PCR and a Rapid, Simple DNA Preparation Protocol
por: Chang, Tsai-De, et al.
Publicado: (2022)

Rapid identification of melioidosis agent by an insulated isothermal PCR on a field–deployable device
por: Chua, Kek Heng, et al.
Publicado: (2020)

Development of a TaqMan Probe-Based Insulated Isothermal Polymerase Chain Reaction (iiPCR) Assay for Detection of Fusarium oxysporum f. sp. cubense Race 4
por: Lin, Ying-Hong, et al.
Publicado: (2016)

A case of transient existence of SARS-CoV-2 RNA in the respiratory tract with the absence of anti-SARS-CoV-2 antibody response
por: Lin, Yi-Chun, et al.
Publicado: (2020)

A field‐deployable insulated isothermal RT‐PCR assay for identification of influenza A (H7N9) shows good performance in the laboratory
por: Inui, Ken, et al.
Publicado: (2019)

Safety and Immunogenicity of a Heterologous Booster of Protein Subunit Vaccine MVC-COV1901 after Two Doses of Adenoviral Vector Vaccine AZD1222
por: Cheng, Shu-Hsing, et al.
Publicado: (2022)

Lopinavir/ritonavir did not shorten the duration of SARS CoV-2 shedding in patients with mild pneumonia in Taiwan
por: Cheng, Chien-Yu, et al.
Publicado: (2020)

Comparison of reverse transcription loop-mediated isothermal amplification, conventional PCR and real-time PCR assays for Japanese encephalitis virus
por: Chen, Zhiyong, et al.
Publicado: (2010)

Development of Colorimetric Reverse Transcription Loop-Mediated Isothermal Amplification Assay for Detecting Feline Coronavirus
por: Rapichai, Witsanu, et al.
Publicado: (2022)

Genetic Authentication of the Medicinal Plant Portulaca oleracea Using a Quick, Precise, and Sensitive Isothermal DNA Amplification Assay
por: Xu, Mo-Rong, et al.
Publicado: (2023)

Rapid and sensitive detection of canine distemper virus by one-tube reverse transcription-insulated isothermal polymerase chain reaction
por: Wilkes, Rebecca P, et al.
Publicado: (2014)

Development of a Duplex Insulated Isothermal PCR Assay for Rapid On-Site Detection and Differentiation of Genotypes 1 and 2 of African Swine Fever Virus
por: Song, Ruilong, et al.
Publicado: (2022)

Rapid and sensitive detection of Mycoplasma synoviae by an insulated isothermal polymerase chain reaction-based assay on a field-deployable device
por: Kuo, Hung-Chih, et al.
Publicado: (2016)

Differential domain structure stability of the severe acute respiratory syndrome coronavirus papain-like protease
por: Chou, Ya-Wen, et al.
Publicado: (2012)

Development and evaluation of a real-time RT-PCR and a field-deployable RT-insulated isothermal PCR for the detection of Seneca Valley virus
por: Zhang, Jianqiang, et al.
Publicado: (2019)

Development of Reverse Transcription Loop-Mediated Isothermal Amplification Assays Targeting Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2)
por: Park, Gun-Soo, et al.
Publicado: (2020)

Incidence and Risk Factors of Reinfection with HCV after Treatment in People Living with HIV
por: Cheng, Chien-Yu, et al.
Publicado: (2022)

Coupling multiplex RT-PCR to a gene chip assay for sensitive and semiquantitative detection of severe acute respiratory syndrome-coronavirus
por: Juang, Jyh-Lyh, et al.
Publicado: (2004)

Rapid Diagnosis of Babesia gibsoni by Point‐of‐Need Testing by Insulated Isothermal PCR in Dogs at High Risk of Infection
por: Cooke, K.L., et al.
Publicado: (2018)

Multicenter study of skin rashes and hepatotoxicity in antiretroviral-naïve HIV-positive patients receiving non-nucleoside reverse-transcriptase inhibitor plus nucleoside reverse-transcriptase inhibitors in Taiwan
por: Wu, Pei-Ying, et al.
Publicado: (2017)

Electromagnetically-driven integrated microfluidic platform using reverse transcription loop-mediated isothermal amplification for detection of severe acute respiratory syndrome coronavirus 2
por: Tsai, Yu-Shiuan, et al.
Publicado: (2022)

Rapid and sensitive detection of PRRSV by a reverse transcription-loop-mediated isothermal amplification assay
por: Zhang, Lei, et al.
Publicado: (2011)

Surveillance of upper respiratory infections using a new multiplex PCR assay compared to conventional methods during the influenza season in Taiwan
por: Chiu, Shu-Chun, et al.
Publicado: (2017)

A fully automated sample-to-answer PCR system for easy and sensitive detection of dengue virus in human serum and mosquitos
por: Tsai, Jih-Jin, et al.
Publicado: (2019)

Diagnostic Devices for Isothermal Nucleic Acid Amplification
por: Chang, Chia-Chen, et al.
Publicado: (2012)

Development of two TaqMan real-time reverse transcription-PCR assays for the detection of severe acute respiratory syndrome coronavirus-2
por: Liu, Yiwei, et al.
Publicado: (2020)

Reverse transcriptase loop-mediated isothermal amplification for the detection of feline coronavirus
por: Stranieri, Angelica, et al.
Publicado: (2017)

Reverse transcription-loop-mediated isothermal amplification for the detection of rodent coronaviruses
por: Hanaki, Ken-Ichi, et al.
Publicado: (2013)

Validation of a Commercial Insulated Isothermal PCR-based POCKIT Test for Rapid and Easy Detection of White Spot Syndrome Virus Infection in Litopenaeus vannamei
por: Tsai, Yun-Long, et al.
Publicado: (2014)

Detection of Middle East respiratory syndrome coronavirus using reverse transcription loop-mediated isothermal amplification (RT-LAMP)
por: Shirato, Kazuya, et al.
Publicado: (2014)

Cannot write session to /tmp/vufind_sessions/sess_vee70cims6tq70faeee3r4n39f