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Comparative performance of CRISPR-Cas12a assays for SARS-CoV-2 detection tested with RNA extracted from clinical specimens
COVID-19 pandemic caused by SARS-CoV-2 infection continue to cause the morbidity and mortality in many countries. Limitations of the gold standard qRT-PCR for diagnosis of this infection includes need for expensive equipment, specialized molecular laboratory, and experienced staff. Currently, CRISPR...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier B.V.
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7849546/ https://www.ncbi.nlm.nih.gov/pubmed/33539846 http://dx.doi.org/10.1016/j.jviromet.2021.114092 |
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author | Nimsamer, Pattaraporn Mayuramart, Oraphan Rattanaburi, Somruthai Chantaravisoot, Naphat Saengchoowong, Suthat Puenpa, Jiratchaya Poovorawan, Yong Payungporn, Sunchai |
author_facet | Nimsamer, Pattaraporn Mayuramart, Oraphan Rattanaburi, Somruthai Chantaravisoot, Naphat Saengchoowong, Suthat Puenpa, Jiratchaya Poovorawan, Yong Payungporn, Sunchai |
author_sort | Nimsamer, Pattaraporn |
collection | PubMed |
description | COVID-19 pandemic caused by SARS-CoV-2 infection continue to cause the morbidity and mortality in many countries. Limitations of the gold standard qRT-PCR for diagnosis of this infection includes need for expensive equipment, specialized molecular laboratory, and experienced staff. Currently, CRISPR-based diagnostic method was approved by the U.S. FDA for rapid detection. Several studies developed SARS-CoV-2 detection based on CRISPR-Cas12a platform; however, the validations with RNA extracted from clinical specimens were limited. Therefore, this study evaluated the clinical performance of previously described CRISPR-Cas12a based diagnostic assays for SARS-CoV-2. According to the results, the CRISPR-Cas12a assays on N1 and S genes provided diagnostic accuracy (≥ 95 %) comparable to the qRT-PCR results. The assays with E, N2 and S genes yielded acceptable sensitivity of detection (≥ 95 %) whereas N1 and S genes provided outstanding specificity of detection (100 %). Preferably, multiple target genes should be detected by using CRISPR-Cas12a to ensure the most effective SARS-CoV-2 detection. Therefore, the N1 and S genes would be attractive target genes for SARS-CoV-2 detection based on CRISPR-Cas12a. |
format | Online Article Text |
id | pubmed-7849546 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Elsevier B.V. |
record_format | MEDLINE/PubMed |
spelling | pubmed-78495462021-02-02 Comparative performance of CRISPR-Cas12a assays for SARS-CoV-2 detection tested with RNA extracted from clinical specimens Nimsamer, Pattaraporn Mayuramart, Oraphan Rattanaburi, Somruthai Chantaravisoot, Naphat Saengchoowong, Suthat Puenpa, Jiratchaya Poovorawan, Yong Payungporn, Sunchai J Virol Methods Short Communication COVID-19 pandemic caused by SARS-CoV-2 infection continue to cause the morbidity and mortality in many countries. Limitations of the gold standard qRT-PCR for diagnosis of this infection includes need for expensive equipment, specialized molecular laboratory, and experienced staff. Currently, CRISPR-based diagnostic method was approved by the U.S. FDA for rapid detection. Several studies developed SARS-CoV-2 detection based on CRISPR-Cas12a platform; however, the validations with RNA extracted from clinical specimens were limited. Therefore, this study evaluated the clinical performance of previously described CRISPR-Cas12a based diagnostic assays for SARS-CoV-2. According to the results, the CRISPR-Cas12a assays on N1 and S genes provided diagnostic accuracy (≥ 95 %) comparable to the qRT-PCR results. The assays with E, N2 and S genes yielded acceptable sensitivity of detection (≥ 95 %) whereas N1 and S genes provided outstanding specificity of detection (100 %). Preferably, multiple target genes should be detected by using CRISPR-Cas12a to ensure the most effective SARS-CoV-2 detection. Therefore, the N1 and S genes would be attractive target genes for SARS-CoV-2 detection based on CRISPR-Cas12a. Elsevier B.V. 2021-04 2021-02-01 /pmc/articles/PMC7849546/ /pubmed/33539846 http://dx.doi.org/10.1016/j.jviromet.2021.114092 Text en © 2021 Elsevier B.V. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active. |
spellingShingle | Short Communication Nimsamer, Pattaraporn Mayuramart, Oraphan Rattanaburi, Somruthai Chantaravisoot, Naphat Saengchoowong, Suthat Puenpa, Jiratchaya Poovorawan, Yong Payungporn, Sunchai Comparative performance of CRISPR-Cas12a assays for SARS-CoV-2 detection tested with RNA extracted from clinical specimens |
title | Comparative performance of CRISPR-Cas12a assays for SARS-CoV-2 detection tested with RNA extracted from clinical specimens |
title_full | Comparative performance of CRISPR-Cas12a assays for SARS-CoV-2 detection tested with RNA extracted from clinical specimens |
title_fullStr | Comparative performance of CRISPR-Cas12a assays for SARS-CoV-2 detection tested with RNA extracted from clinical specimens |
title_full_unstemmed | Comparative performance of CRISPR-Cas12a assays for SARS-CoV-2 detection tested with RNA extracted from clinical specimens |
title_short | Comparative performance of CRISPR-Cas12a assays for SARS-CoV-2 detection tested with RNA extracted from clinical specimens |
title_sort | comparative performance of crispr-cas12a assays for sars-cov-2 detection tested with rna extracted from clinical specimens |
topic | Short Communication |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7849546/ https://www.ncbi.nlm.nih.gov/pubmed/33539846 http://dx.doi.org/10.1016/j.jviromet.2021.114092 |
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