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Comparative performance of CRISPR-Cas12a assays for SARS-CoV-2 detection tested with RNA extracted from clinical specimens

COVID-19 pandemic caused by SARS-CoV-2 infection continue to cause the morbidity and mortality in many countries. Limitations of the gold standard qRT-PCR for diagnosis of this infection includes need for expensive equipment, specialized molecular laboratory, and experienced staff. Currently, CRISPR...

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Autores principales: Nimsamer, Pattaraporn, Mayuramart, Oraphan, Rattanaburi, Somruthai, Chantaravisoot, Naphat, Saengchoowong, Suthat, Puenpa, Jiratchaya, Poovorawan, Yong, Payungporn, Sunchai
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier B.V. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7849546/
https://www.ncbi.nlm.nih.gov/pubmed/33539846
http://dx.doi.org/10.1016/j.jviromet.2021.114092
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author Nimsamer, Pattaraporn
Mayuramart, Oraphan
Rattanaburi, Somruthai
Chantaravisoot, Naphat
Saengchoowong, Suthat
Puenpa, Jiratchaya
Poovorawan, Yong
Payungporn, Sunchai
author_facet Nimsamer, Pattaraporn
Mayuramart, Oraphan
Rattanaburi, Somruthai
Chantaravisoot, Naphat
Saengchoowong, Suthat
Puenpa, Jiratchaya
Poovorawan, Yong
Payungporn, Sunchai
author_sort Nimsamer, Pattaraporn
collection PubMed
description COVID-19 pandemic caused by SARS-CoV-2 infection continue to cause the morbidity and mortality in many countries. Limitations of the gold standard qRT-PCR for diagnosis of this infection includes need for expensive equipment, specialized molecular laboratory, and experienced staff. Currently, CRISPR-based diagnostic method was approved by the U.S. FDA for rapid detection. Several studies developed SARS-CoV-2 detection based on CRISPR-Cas12a platform; however, the validations with RNA extracted from clinical specimens were limited. Therefore, this study evaluated the clinical performance of previously described CRISPR-Cas12a based diagnostic assays for SARS-CoV-2. According to the results, the CRISPR-Cas12a assays on N1 and S genes provided diagnostic accuracy (≥ 95 %) comparable to the qRT-PCR results. The assays with E, N2 and S genes yielded acceptable sensitivity of detection (≥ 95 %) whereas N1 and S genes provided outstanding specificity of detection (100 %). Preferably, multiple target genes should be detected by using CRISPR-Cas12a to ensure the most effective SARS-CoV-2 detection. Therefore, the N1 and S genes would be attractive target genes for SARS-CoV-2 detection based on CRISPR-Cas12a.
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spelling pubmed-78495462021-02-02 Comparative performance of CRISPR-Cas12a assays for SARS-CoV-2 detection tested with RNA extracted from clinical specimens Nimsamer, Pattaraporn Mayuramart, Oraphan Rattanaburi, Somruthai Chantaravisoot, Naphat Saengchoowong, Suthat Puenpa, Jiratchaya Poovorawan, Yong Payungporn, Sunchai J Virol Methods Short Communication COVID-19 pandemic caused by SARS-CoV-2 infection continue to cause the morbidity and mortality in many countries. Limitations of the gold standard qRT-PCR for diagnosis of this infection includes need for expensive equipment, specialized molecular laboratory, and experienced staff. Currently, CRISPR-based diagnostic method was approved by the U.S. FDA for rapid detection. Several studies developed SARS-CoV-2 detection based on CRISPR-Cas12a platform; however, the validations with RNA extracted from clinical specimens were limited. Therefore, this study evaluated the clinical performance of previously described CRISPR-Cas12a based diagnostic assays for SARS-CoV-2. According to the results, the CRISPR-Cas12a assays on N1 and S genes provided diagnostic accuracy (≥ 95 %) comparable to the qRT-PCR results. The assays with E, N2 and S genes yielded acceptable sensitivity of detection (≥ 95 %) whereas N1 and S genes provided outstanding specificity of detection (100 %). Preferably, multiple target genes should be detected by using CRISPR-Cas12a to ensure the most effective SARS-CoV-2 detection. Therefore, the N1 and S genes would be attractive target genes for SARS-CoV-2 detection based on CRISPR-Cas12a. Elsevier B.V. 2021-04 2021-02-01 /pmc/articles/PMC7849546/ /pubmed/33539846 http://dx.doi.org/10.1016/j.jviromet.2021.114092 Text en © 2021 Elsevier B.V. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Short Communication
Nimsamer, Pattaraporn
Mayuramart, Oraphan
Rattanaburi, Somruthai
Chantaravisoot, Naphat
Saengchoowong, Suthat
Puenpa, Jiratchaya
Poovorawan, Yong
Payungporn, Sunchai
Comparative performance of CRISPR-Cas12a assays for SARS-CoV-2 detection tested with RNA extracted from clinical specimens
title Comparative performance of CRISPR-Cas12a assays for SARS-CoV-2 detection tested with RNA extracted from clinical specimens
title_full Comparative performance of CRISPR-Cas12a assays for SARS-CoV-2 detection tested with RNA extracted from clinical specimens
title_fullStr Comparative performance of CRISPR-Cas12a assays for SARS-CoV-2 detection tested with RNA extracted from clinical specimens
title_full_unstemmed Comparative performance of CRISPR-Cas12a assays for SARS-CoV-2 detection tested with RNA extracted from clinical specimens
title_short Comparative performance of CRISPR-Cas12a assays for SARS-CoV-2 detection tested with RNA extracted from clinical specimens
title_sort comparative performance of crispr-cas12a assays for sars-cov-2 detection tested with rna extracted from clinical specimens
topic Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7849546/
https://www.ncbi.nlm.nih.gov/pubmed/33539846
http://dx.doi.org/10.1016/j.jviromet.2021.114092
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