Diagnosis of COVID-19 using multiple antibody assays in two cases with negative PCR results from nasopharyngeal swabs

We report of two cases of progressed COVID-19 with negative PCR tests from nasopharyngeal swabs, in whom diagnosis was made by different antibody assays, including a lateral flow rapid test and multiple commercial ELISAs, finally confirmed by comprehensive serological assays. These cases highlight t...

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Autores principales: Traugott, Marianna Theresia, Hoepler, Wolfgang, Seitz, Tamara, Baumgartner, Sebastian, Karolyi, Mario, Pawelka, Erich, Friese, Emanuela, Neuhold, Stephanie, Kelani, Hasan, Thalhammer, Florian, Zoufaly, Alexander, Laferl, Hermann, Aberle, Judith Helene, Wenisch, Christoph, Puchhammer-Stöckl, Elisabeth, Stiasny, Karin, Aberle, Stephan Walter, Weseslindtner, Lukas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2020
Materias:
Case Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7851003/
https://www.ncbi.nlm.nih.gov/pubmed/32785885
http://dx.doi.org/10.1007/s15010-020-01497-2
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author Traugott, Marianna Theresia
Hoepler, Wolfgang
Seitz, Tamara
Baumgartner, Sebastian
Karolyi, Mario
Pawelka, Erich
Friese, Emanuela
Neuhold, Stephanie
Kelani, Hasan
Thalhammer, Florian
Zoufaly, Alexander
Laferl, Hermann
Aberle, Judith Helene
Wenisch, Christoph
Puchhammer-Stöckl, Elisabeth
Stiasny, Karin
Aberle, Stephan Walter
Weseslindtner, Lukas
author_facet Traugott, Marianna Theresia
Hoepler, Wolfgang
Seitz, Tamara
Baumgartner, Sebastian
Karolyi, Mario
Pawelka, Erich
Friese, Emanuela
Neuhold, Stephanie
Kelani, Hasan
Thalhammer, Florian
Zoufaly, Alexander
Laferl, Hermann
Aberle, Judith Helene
Wenisch, Christoph
Puchhammer-Stöckl, Elisabeth
Stiasny, Karin
Aberle, Stephan Walter
Weseslindtner, Lukas
author_sort Traugott, Marianna Theresia
collection PubMed
description We report of two cases of progressed COVID-19 with negative PCR tests from nasopharyngeal swabs, in whom diagnosis was made by different antibody assays, including a lateral flow rapid test and multiple commercial ELISAs, finally confirmed by comprehensive serological assays. These cases highlight that commercial ELISAs and even rapid tests might significantly aid the diagnosis of COVID-19, particularly, if a combination of serological assays is used with a specific clinical question, in severely ill patients after seroconversion and when comprehensive serological methods are used for confirmation. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s15010-020-01497-2) contains supplementary material, which is available to authorized users.
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spelling pubmed-78510032021-02-08 Diagnosis of COVID-19 using multiple antibody assays in two cases with negative PCR results from nasopharyngeal swabs Traugott, Marianna Theresia Hoepler, Wolfgang Seitz, Tamara Baumgartner, Sebastian Karolyi, Mario Pawelka, Erich Friese, Emanuela Neuhold, Stephanie Kelani, Hasan Thalhammer, Florian Zoufaly, Alexander Laferl, Hermann Aberle, Judith Helene Wenisch, Christoph Puchhammer-Stöckl, Elisabeth Stiasny, Karin Aberle, Stephan Walter Weseslindtner, Lukas Infection Case Report We report of two cases of progressed COVID-19 with negative PCR tests from nasopharyngeal swabs, in whom diagnosis was made by different antibody assays, including a lateral flow rapid test and multiple commercial ELISAs, finally confirmed by comprehensive serological assays. These cases highlight that commercial ELISAs and even rapid tests might significantly aid the diagnosis of COVID-19, particularly, if a combination of serological assays is used with a specific clinical question, in severely ill patients after seroconversion and when comprehensive serological methods are used for confirmation. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s15010-020-01497-2) contains supplementary material, which is available to authorized users. Springer Berlin Heidelberg 2020-08-12 2021 /pmc/articles/PMC7851003/ /pubmed/32785885 http://dx.doi.org/10.1007/s15010-020-01497-2 Text en © The Author(s) 2020 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Case Report
Traugott, Marianna Theresia
Hoepler, Wolfgang
Seitz, Tamara
Baumgartner, Sebastian
Karolyi, Mario
Pawelka, Erich
Friese, Emanuela
Neuhold, Stephanie
Kelani, Hasan
Thalhammer, Florian
Zoufaly, Alexander
Laferl, Hermann
Aberle, Judith Helene
Wenisch, Christoph
Puchhammer-Stöckl, Elisabeth
Stiasny, Karin
Aberle, Stephan Walter
Weseslindtner, Lukas
Diagnosis of COVID-19 using multiple antibody assays in two cases with negative PCR results from nasopharyngeal swabs
title Diagnosis of COVID-19 using multiple antibody assays in two cases with negative PCR results from nasopharyngeal swabs
title_full Diagnosis of COVID-19 using multiple antibody assays in two cases with negative PCR results from nasopharyngeal swabs
title_fullStr Diagnosis of COVID-19 using multiple antibody assays in two cases with negative PCR results from nasopharyngeal swabs
title_full_unstemmed Diagnosis of COVID-19 using multiple antibody assays in two cases with negative PCR results from nasopharyngeal swabs
title_short Diagnosis of COVID-19 using multiple antibody assays in two cases with negative PCR results from nasopharyngeal swabs
title_sort diagnosis of covid-19 using multiple antibody assays in two cases with negative pcr results from nasopharyngeal swabs
topic Case Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7851003/
https://www.ncbi.nlm.nih.gov/pubmed/32785885
http://dx.doi.org/10.1007/s15010-020-01497-2
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