Bisulfite-free epigenomics and genomics of single cells through methylation-sensitive restriction

Single-cell multi-omics are powerful means to study cell-to-cell heterogeneity. Here, we present a single-tube, bisulfite-free method for the simultaneous, genome-wide analysis of DNA methylation and genetic variants in single cells: epigenomics and genomics of single cells analyzed by restriction (...

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Detalles Bibliográficos
Autores principales: Niemöller, Christoph, Wehrle, Julius, Riba, Julian, Claus, Rainer, Renz, Nathalie, Rhein, Janika, Bleul, Sabine, Stosch, Juliane M., Duyster, Justus, Plass, Christoph, Lutsik, Pavlo, Lipka, Daniel B., Lübbert, Michael, Becker, Heiko
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7851132/
https://www.ncbi.nlm.nih.gov/pubmed/33526904
http://dx.doi.org/10.1038/s42003-021-01661-w
Descripción
Sumario:Single-cell multi-omics are powerful means to study cell-to-cell heterogeneity. Here, we present a single-tube, bisulfite-free method for the simultaneous, genome-wide analysis of DNA methylation and genetic variants in single cells: epigenomics and genomics of single cells analyzed by restriction (epi-gSCAR). By applying this method, we obtained DNA methylation measurements of up to 506,063 CpGs and up to 1,244,188 single-nucleotide variants from single acute myeloid leukemia-derived cells. We demonstrate that epi-gSCAR generates accurate and reproducible measurements of DNA methylation and allows to differentiate between cell lines based on the DNA methylation and genetic profiles.

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