Bisulfite-free epigenomics and genomics of single cells through methylation-sensitive restriction

Single-cell multi-omics are powerful means to study cell-to-cell heterogeneity. Here, we present a single-tube, bisulfite-free method for the simultaneous, genome-wide analysis of DNA methylation and genetic variants in single cells: epigenomics and genomics of single cells analyzed by restriction (...

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Autores principales: Niemöller, Christoph, Wehrle, Julius, Riba, Julian, Claus, Rainer, Renz, Nathalie, Rhein, Janika, Bleul, Sabine, Stosch, Juliane M., Duyster, Justus, Plass, Christoph, Lutsik, Pavlo, Lipka, Daniel B., Lübbert, Michael, Becker, Heiko
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7851132/
https://www.ncbi.nlm.nih.gov/pubmed/33526904
http://dx.doi.org/10.1038/s42003-021-01661-w
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author Niemöller, Christoph
Wehrle, Julius
Riba, Julian
Claus, Rainer
Renz, Nathalie
Rhein, Janika
Bleul, Sabine
Stosch, Juliane M.
Duyster, Justus
Plass, Christoph
Lutsik, Pavlo
Lipka, Daniel B.
Lübbert, Michael
Becker, Heiko
author_facet Niemöller, Christoph
Wehrle, Julius
Riba, Julian
Claus, Rainer
Renz, Nathalie
Rhein, Janika
Bleul, Sabine
Stosch, Juliane M.
Duyster, Justus
Plass, Christoph
Lutsik, Pavlo
Lipka, Daniel B.
Lübbert, Michael
Becker, Heiko
author_sort Niemöller, Christoph
collection PubMed
description Single-cell multi-omics are powerful means to study cell-to-cell heterogeneity. Here, we present a single-tube, bisulfite-free method for the simultaneous, genome-wide analysis of DNA methylation and genetic variants in single cells: epigenomics and genomics of single cells analyzed by restriction (epi-gSCAR). By applying this method, we obtained DNA methylation measurements of up to 506,063 CpGs and up to 1,244,188 single-nucleotide variants from single acute myeloid leukemia-derived cells. We demonstrate that epi-gSCAR generates accurate and reproducible measurements of DNA methylation and allows to differentiate between cell lines based on the DNA methylation and genetic profiles.
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spelling pubmed-78511322021-02-08 Bisulfite-free epigenomics and genomics of single cells through methylation-sensitive restriction Niemöller, Christoph Wehrle, Julius Riba, Julian Claus, Rainer Renz, Nathalie Rhein, Janika Bleul, Sabine Stosch, Juliane M. Duyster, Justus Plass, Christoph Lutsik, Pavlo Lipka, Daniel B. Lübbert, Michael Becker, Heiko Commun Biol Article Single-cell multi-omics are powerful means to study cell-to-cell heterogeneity. Here, we present a single-tube, bisulfite-free method for the simultaneous, genome-wide analysis of DNA methylation and genetic variants in single cells: epigenomics and genomics of single cells analyzed by restriction (epi-gSCAR). By applying this method, we obtained DNA methylation measurements of up to 506,063 CpGs and up to 1,244,188 single-nucleotide variants from single acute myeloid leukemia-derived cells. We demonstrate that epi-gSCAR generates accurate and reproducible measurements of DNA methylation and allows to differentiate between cell lines based on the DNA methylation and genetic profiles. Nature Publishing Group UK 2021-02-01 /pmc/articles/PMC7851132/ /pubmed/33526904 http://dx.doi.org/10.1038/s42003-021-01661-w Text en © The Author(s) 2021 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Niemöller, Christoph
Wehrle, Julius
Riba, Julian
Claus, Rainer
Renz, Nathalie
Rhein, Janika
Bleul, Sabine
Stosch, Juliane M.
Duyster, Justus
Plass, Christoph
Lutsik, Pavlo
Lipka, Daniel B.
Lübbert, Michael
Becker, Heiko
Bisulfite-free epigenomics and genomics of single cells through methylation-sensitive restriction
title Bisulfite-free epigenomics and genomics of single cells through methylation-sensitive restriction
title_full Bisulfite-free epigenomics and genomics of single cells through methylation-sensitive restriction
title_fullStr Bisulfite-free epigenomics and genomics of single cells through methylation-sensitive restriction
title_full_unstemmed Bisulfite-free epigenomics and genomics of single cells through methylation-sensitive restriction
title_short Bisulfite-free epigenomics and genomics of single cells through methylation-sensitive restriction
title_sort bisulfite-free epigenomics and genomics of single cells through methylation-sensitive restriction
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7851132/
https://www.ncbi.nlm.nih.gov/pubmed/33526904
http://dx.doi.org/10.1038/s42003-021-01661-w
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