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LINC01224 Promotes Colorectal Cancer Progression by Sponging miR-2467

INTRODUCTION: Colorectal cancer (CRC) is one of the most common human cancers and a leading cause of cancer-related death. Accumulating evidence has confirmed that long non-coding RNA (lncRNA) plays crucial roles in CRC development. METHODS: qRT-PCR was performed to examine the expressions of LINC01...

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Autores principales: Chen, Lin, Chen, Wei, Zhao, Changjie, Jiang, Qi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7851399/
https://www.ncbi.nlm.nih.gov/pubmed/33542656
http://dx.doi.org/10.2147/CMAR.S281625
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author Chen, Lin
Chen, Wei
Zhao, Changjie
Jiang, Qi
author_facet Chen, Lin
Chen, Wei
Zhao, Changjie
Jiang, Qi
author_sort Chen, Lin
collection PubMed
description INTRODUCTION: Colorectal cancer (CRC) is one of the most common human cancers and a leading cause of cancer-related death. Accumulating evidence has confirmed that long non-coding RNA (lncRNA) plays crucial roles in CRC development. METHODS: qRT-PCR was performed to examine the expressions of LINC01224 and miR-2467. CCK-8 assay, colony formation assay and transwell invasion assay were used to examine the progression of breast cancer cells. Luciferase and RNA-binding protein immunoprecipitation (RIP) assay were applied to verify the binding site. Correlation analysis of miR-2467 and LINC01224 expression in lung cancer tissues was shown. Pancreatic cancer cells growth in vivo was evaluated using xenograft tumor assay. RESULTS: LINC01224 expression was observed to be up-regulated in CRC tissues and cell lines. Functional studies suggested that LINC01224 silence inhibited CRC cells proliferation and invasion of CRC cells, while co-transfection with a miR-2467 inhibitor reversed these biological effects. Luciferase reporter assays illustrated that LINC01224 regulated miR-2467 directly, and RNA-binding protein immunoprecipitation (RIP) further confirmed that the suppression of LINC01224 by miR-2467 was in an RISC-dependent manner. Finally, LINC01224 silence inhibited the growth CRC cells in vivo. CONCLUSION: In conclusion, our findings showed that LINC01224 promoted CRC progression through sponging miR-2467. LINC01224 may serve as a potential diagnostic biomarker and therapeutic target for CRC patients.
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spelling pubmed-78513992021-02-03 LINC01224 Promotes Colorectal Cancer Progression by Sponging miR-2467 Chen, Lin Chen, Wei Zhao, Changjie Jiang, Qi Cancer Manag Res Original Research INTRODUCTION: Colorectal cancer (CRC) is one of the most common human cancers and a leading cause of cancer-related death. Accumulating evidence has confirmed that long non-coding RNA (lncRNA) plays crucial roles in CRC development. METHODS: qRT-PCR was performed to examine the expressions of LINC01224 and miR-2467. CCK-8 assay, colony formation assay and transwell invasion assay were used to examine the progression of breast cancer cells. Luciferase and RNA-binding protein immunoprecipitation (RIP) assay were applied to verify the binding site. Correlation analysis of miR-2467 and LINC01224 expression in lung cancer tissues was shown. Pancreatic cancer cells growth in vivo was evaluated using xenograft tumor assay. RESULTS: LINC01224 expression was observed to be up-regulated in CRC tissues and cell lines. Functional studies suggested that LINC01224 silence inhibited CRC cells proliferation and invasion of CRC cells, while co-transfection with a miR-2467 inhibitor reversed these biological effects. Luciferase reporter assays illustrated that LINC01224 regulated miR-2467 directly, and RNA-binding protein immunoprecipitation (RIP) further confirmed that the suppression of LINC01224 by miR-2467 was in an RISC-dependent manner. Finally, LINC01224 silence inhibited the growth CRC cells in vivo. CONCLUSION: In conclusion, our findings showed that LINC01224 promoted CRC progression through sponging miR-2467. LINC01224 may serve as a potential diagnostic biomarker and therapeutic target for CRC patients. Dove 2021-01-26 /pmc/articles/PMC7851399/ /pubmed/33542656 http://dx.doi.org/10.2147/CMAR.S281625 Text en © 2021 Chen et al. http://creativecommons.org/licenses/by-nc/3.0/ This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php).
spellingShingle Original Research
Chen, Lin
Chen, Wei
Zhao, Changjie
Jiang, Qi
LINC01224 Promotes Colorectal Cancer Progression by Sponging miR-2467
title LINC01224 Promotes Colorectal Cancer Progression by Sponging miR-2467
title_full LINC01224 Promotes Colorectal Cancer Progression by Sponging miR-2467
title_fullStr LINC01224 Promotes Colorectal Cancer Progression by Sponging miR-2467
title_full_unstemmed LINC01224 Promotes Colorectal Cancer Progression by Sponging miR-2467
title_short LINC01224 Promotes Colorectal Cancer Progression by Sponging miR-2467
title_sort linc01224 promotes colorectal cancer progression by sponging mir-2467
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7851399/
https://www.ncbi.nlm.nih.gov/pubmed/33542656
http://dx.doi.org/10.2147/CMAR.S281625
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